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β-adrenergic receptor regulation, through cyclic AMP, of nerve growth factor expression in rat cortical and cerebellar astrocytes

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Summary

  1. 1.

    Type 1 astrocytes prepared from 3-day rat cortex and cerebellum express the 1.3-kb nerve growth factor (NGF) mRNA and synthesize and releaseβ-NGF.

  2. 2.

    Isoproterenol (IP), aβ-adrenergic agonist, stimulates NGF mRNA content in cortical astrocytes; this increase is blocked by theβ-adrenergic antagonist propranolol but not theα-antagonist phenoxybenzamine. The EC50 for the effect of IP is 5 nM.

  3. 3.

    IP increases astrocyte cyclic AMP as does forskolin, which directly activates adenylate cyclase and also increases NGF mRNA content. Cerebellar astrocytes contain about one-third as much NGF mRNA, which can also be increased by forskolin and cyclic AMP.

  4. 4.

    These results suggest that CNS astrocytes can serve as a source of NGF and that the NGF gene is one of the class of cyclic AMP regulated genes.

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  1. Clinical Neuroscience Branch, National Institute of Neurological Disorders and Stroke, National Institutes of Health, 20892, Bethesda, Maryland, USA

    Joan P. Schwartz & Kathleen Mishler

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  1. Joan P. Schwartz
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  2. Kathleen Mishler
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Schwartz, J.P., Mishler, K. β-adrenergic receptor regulation, through cyclic AMP, of nerve growth factor expression in rat cortical and cerebellar astrocytes. Cell Mol Neurobiol 10, 447–457 (1990). https://doi.org/10.1007/BF00711186

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  • DOI: https://doi.org/10.1007/BF00711186

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