Summary
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1.
Replication of DNA in the epidermis of the crayfish was found to be correlated with the molting cycle. A distinct14C-thymidine incorporation peak was found in mid-premolt stages D1″ and D1‴.
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2.
Injected ecdysterone (2 μg/g body weight) had effects on DNA synthesis which varied with the stages of the molting cycle. It stimulated14C-thymidine incorporation in postmolt stage C1, intermolt stages C3−C4, and premolt stage D1′, but had no significant effect during any subsequent premolt stage and during early postmolt stage B. Ecdysterone induced a peak at stage D1′ that was essentially equal in magnitude to the normal peak at stage D1‴.
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References
Carlisle, D. B., Connick, R. O.: Crustecdysone (20-hydroxyecdysone): site of storage in the crayfishOrconectes propinquus. Canad. J. Zool.51, 417–420 (1973)
Ceriotti, G.: A microchemical determination of deoxyribonucleic acid. J. Biol. Chem.198, 297–303 (1952)
Davidson, J. N.: The biochemistry of nucleic acids, p. 20. New York: Barnes and Noble 1965
Dennell, R.: Integument and exoskeleton. In: The physiology of crustacea (T. H. Waterman, ed.), vol. 1: Metabolism and growth, p. 449–472. New York: Academic Press 1960
Drach, P.: Mue et cycle d'intermue chez les crustacés décapodes. Ann. Inst. Oceanorgr., Monaco19, 103–391 (1939)
Emmerich, H.: Ecdysone binding proteins in nuclei and chromatin fromDrosophila salivary glands. Gen. comp. Endocr.19, 543–551 (1972)
Gorell, T. A., Gilbert, L. I., Sidall, J. B.: Binding proteins for an ecdysone metabolite in the crustacean hepatopancreas. Proc. nat. Acad. Sci. (Wash.)69, 812–815 (1972)
Humphreys, C. R., Stevenson, J. R.: Changes in epidermal DNA, protein and protein synthesis during the molt cycle of the crayfishOrconectes sanborni (Faxon). Comp. Biochem. Physiol.44A, 1121–1128 (1973)
Keller, R., Adelung, D.: Vergleichende morphologische und physiologische Untersuchungen des Integumentgewebes und des Häutungshormongehaltes beim FlußkrebsOrconectes limosus während eines Häutungszyklus. Wilhelm Roux' Archiv164, 209–221 (1970)
Krishnakumaran, A., Schneiderman, H. A.: Control of molting in mandibulate and chelicerate arthropods by ecdysone. Biol. Bull.139, 520–538 (1970)
Madhaven, K., Schneiderman, H. A.: Hormonal control of imaginal disk regeneration inGalleria mellonella. Biol. Bull.137, 321–331 (1969)
Maissiat, J., Legrand, J. J.: Contribution à l'étude expérimentale du contrôle hormonal du cycle de mue chez les OniscoïdesPorcellio dilatatus etLigia oceania. C. R. Soc. Biol. (Paris)164, 359–362 (1970)
Markham, R., Smith, J. D.: Chromatographic studies of the nucleic acids. Biochem. J.45, 294–298 (1949)
Ohmori, K., Ohtaki, T.: Effects of ecdysone analogues on development and metabolic activity of wing disks of the fleshfly,Sarcophaga peregrina, in vitro. J. Insect Physiol.19, 1199–1210 (1973)
Passano, L. M.: Molting and its control. In: Physiology of crustacea (T. H. Waterman ed.), vol. 1: Metabolism and growth, p. 475–536. New York: Academic Press 1960
Postlethwait, J. H., Schneiderman, H. A.: Effects of an ecdysone on growth and cuticle formation ofDrosophila imaginal discs cultured in vivo. Biol. Bull.135 431–432 (1968)
Rao, K. R., Fingerman, S. W., Fingerman, M.: Effects of exogenous ecdysones on the molt cycles of fourth and fifth stage American lobsters,Homarus americanus. Comp. Biochem. Physiol.44A, 1105–1120 (1973)
Skinner, D. M.: The structure and metabolism of a crustacean integumentary tissue during a molt cycle. Biol. Bull.123, 635–647 (1962)
Skinner, D. M.: Amino acid incorporation into protein during the molt cycle of the land crabGecarcinus lateralis. J. exp. Zool.160, 225–234 (1965)
Stevenson, J. R.: Metecdysial molt staging and changes in the cuticle in the crayfishOrconectes sanborni (Faxon). Crustaceana14 (Part 2), 169–177 (1968)
Stevenson, J. R., Guckert, R. H., Cohen, J. D.: Lack of correlation of some proecdysial growth and developmental processes in the crayfish. Biol. Bull.134, 160–175 (1968)
Stevenson, J. R., Tschantz, J. A.: Acceleration by ecdysterone of premolt substages in the crayfish. Nature (Lond.)242, 133–134 (1973)
Tchernigovtzeff, C.: Croissance épithéliale et cycle d'intermue chezLeander serratus (Pennant). C. R. Acad. Sci. (Paris)248, 600–602 (1959)
Travis, D. F.: The molting cycle of the spiny lobster,Panulirus argus Latreille—II. Preecdysial histological and histochemical changes in the hepato-pancreas and integumental tissues. Biol. Bull.108, 88–112 (1955)
Van Harreveld, A.: A physiological solution for freshwater crustaceans. Proc. Soc. exp. Biol. (N.Y.)34, 428–432 (1936)
Vijverberg, A. J.: Incorporation of tritiated thymidine in the wing and leg disks ofCalliphora erythrocephala Meigen. Short term effect of ecdysterone on DNA synthesis during larval and prepupal development. Netherl. J. Zool.23, 189–214 (1973)
Warner, A. C., Stevenson, J. R.: The influence of ecdysones and eyestalk removal on the molt cycle of the crayfishOrconectes obscurus. Gen. comp. Endocr.18, 454–462 (1972)
Willig, A., Keller, R.: Molting hormone content, cuticle growth and gastrolith growth in the molt cycle of the crayfish,Orconectes limosus. J. comp. Physiol.86 377–388 (1973)
Wyatt, G. R.: Hormone action and macromolecule biosynthesis in silkmoth pupae. Endocr. exp.5 91–95 (1971)
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Wittig, K., Stevenson, J.R. DNA synthesis in the crayfish epidermis and its modification by ecdysterone. J Comp Physiol B 99, 279–286 (1975). https://doi.org/10.1007/BF00710368
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DOI: https://doi.org/10.1007/BF00710368