Summary
The distribution of serotonin immunoreactivity in the superior colliculus (SC) of the rat, hamster, chipmunk, cat, and monkey was studied using a sensitive immunohistochemical method. In all of these animals, serotonin immunoreactivity formed a dense network of varicose fibers throughout the SC. These fibers had a characteristic arrangement corresponding to the laminar structures of the SC. Except in the chipmunk, serotonergic fibers were more dense in the stratum griseum superficiale than in the other layers. In the SC of the chipmunk, these fibers appeared evenly distributed.
To explore the degree of scrotonergic innervation in each layer, a semi-quantitative assay of serotonin immunoreactive varicosities was conducted in the rat, chipmunk, cat, and monkey. Peaks in varicose density were seen in the stratum griseum superficiale, the stratum griseum intermedium and the stratum griseum profundum. In the rat, cat, and monkey, the highest density of these varicosities was in the stratum griseum superficiale. On the other hand, the stratum griseum intermedium of the chipmunk SC received the greatest innervation of serotonergic varicose fibers.
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References
Casagrande VA, Harting JK, Hall WC, Diamond IT (1972) Superior colliculus of the tree shrew: a structural and functional subdivision into superficial and deep layers. Science 177:444–447
Chan-Palay V (1977) Indoleamine neurons and their processes in the normal rat brain and chronic diet-induced thiamine deficiency demonstrated by uptake of3H-serotonin. J Comp Neurol 176:467–494
Edwards S, Ginsburgh CL, Henkel CK, Stein BE (1979) Sources of subcortical projections to the superior colliculus in the cat. J Comp Neurol 184:309–330
Fuxe K (1965) Evidence for the existence of monoamine neurons in the central nervous system. IV the distribution of monoamine terminals in the central nervous system. Acta Physiol Scand 64, Suppl 247:38–85
Graham J (1977) An autoradiographic study of the efferent connections of the superior colliculus of the cat. J Comp Neurol 173:629–654
Graybiel AM (1978) Organization of the nigrotectal connection: an experimental tracer study in the cat. Brain Res 143:339–348
Graybiel AM, Brecha N, Karten HJ (1984) Cluster-and-sheet pattern of enkephalin-like immunoreactivities in the superior colliculus of the cat. Neuroscience 12:191–214
Kawai N (1970) Release of 5-hydroxytryptamine from slices of superior colliculus by optic tract stimulation. Neuropharmacology 9:395–397
Kawai N, Yamamoto C (1969) Effects of 5-hydroxytryptamine, LSD and related compounds on electrical activities evokted in vitro in the sections from the superior collicuclus. Int J Neurophalmac 8:437–449
May PJ, Hall WC (1984) Relationships between the nigrotectal pathway and the cells of origin of the predorsal bundle. J Comp Neurol 226:357–376
Palkovits M, Brownstein M, Saavedra JM (1974) Serotonin content of the brain stem nuclei in the rat. Brain Res 80:237–249
Parent A, Descarries L, Beaudet A (1981) Organization of ascending serotonin systems in the adult rat brain. A radioautographic study after intraventricular administration of [3H]5-hydroxytryptamine. Neuroscience 6:115–138
Sano Y, Takeuchi Y, Kimura H, Goto M, Kawata M, Kojima M, Matsuura T, Ueda S, Yamada H (1982) Immunohistochemical studies on the processes of serotonin neurons and their ramification in the central nervous system-with regard to the possibility of the existence of GOLGI's rete nervosa diffusa. Arch Histol Jpn 45:305–316
Steinbuch HWM (1981) Distribution of serotonin-immunoreactivity in the central nervous system of the rat-cell bodies and terminals. Neuroscience 6:557–618
Straschill M, Perwein J (1971) Effect of iontophoretically applied biogenic amines and cholinomimetic substances upon the activity of neurons in the superior colliculus and mesencephalic reticular formation of the cat. Pflügers Arch 324:43–55
Takeuchi Y, Sano Y (1984) Serotonin nerve fibers in the primary visual cortex of the monkey. Quantitative and immunoelectronmicroscopical analysis. Anat Embryol 169:1–8
Takeuchi Y, Kimura H, Sano Y (1982) Immunohistochemical demonstration of serotonin neurons in the brainstem of the rat and cat. Cell Tissue Res 224:247–267
Ueda S, Nojyo Y, Sano Y (1984) Immunohistochemical demonstration of the serotonin neuron system in the central nervous system of the bullfrog,Rana catesbeiana. Anat Embryol 169:219–229
Ueda S, Takeuchi Y, Sano Y (1983) Immunohistochemical demonstration of serotonin neurons in the central nervous system of the turtle (Clemmys japonica). Anat Embryol 168:1–19
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Supported by a grant from the Ministry of Education, Science, and Culture of Japan (No. 57214028)
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Ueda, S., Ihara, N. & Sano, Y. The organization of serotonin fibers in the mammalian superior colliculus. Anat Embryol 173, 13–21 (1985). https://doi.org/10.1007/BF00707300
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DOI: https://doi.org/10.1007/BF00707300