Summary
The incorporation of35S-l-cysteine into the hypothalamic-hypophyseal neurosecretory system was studied in normal male albino rats and in similar rats which had received thiouracil or excess thyroxine during 18 days. The study was performed by autoradiography. Labelled cysteine was administered by intraperitoneal injections. The animals were sacrificed 30 min, 3 and 24 hours after injection. From the autoradiographs the grain counts were determined in the supraoptic nucleus, paraventricular nucleus, median eminence and infundibular process. The thyroxine-treated rats in the 30 minute group displayed a statistically almost significantly higher grain count in the supraoptic nucleus than the corresponding controls; the same was observed in the 3 hour group in the median eminence and infundibular process. In the 24 hour group, however, the grain count was clearly lower at all sites examined than in the controls. With the rats that had been under thiouracil treatment statistically significant differences were only established in the 24 hour group. Their grain count was higher at all sites than that of the corresponding controls. From the results the inference is drawn that thyroxine accelerates the protein synthesis in the ganglion cells of the supraoptic nucleus and accelerates the flow of NSM to the infundibular process and its release there into the blood flow. Thiouracil, again, retards the flow and the release.
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This work was supported by a grant from the Sigrid Jusélius Foundation, Helsinki.
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Talanti, S., Pasanen, V. The incorporation of35S-labelled cysteine in the hypothalamic-hypophyseal neurosecretory system of rats treated with thiouracil and excess thyroxine. Z.Zellforsch 88, 220–227 (1968). https://doi.org/10.1007/BF00703909
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DOI: https://doi.org/10.1007/BF00703909