Summary
Cell surface sugar residues in neurulating ectoderms of bantam chick embryos of stage 4–11 were examined using ferritin-labeledRicinus communis (RCAI) and Wheat germ agglutinin (WGA). RCAI binding sites densely covered the apical surfaces of the basal plate cells during the neural plate stage (1,322.8±28.8 ferritin particles/μm2). As neural tube formation advanced, the number of receptors decreased as a result of an increase in the extent of the sparsely covered regions. The decrease in receptors for WGA occurred in a similar manner but more rapidly. By the stage of development at which the opposite sides of the neural ridges meet at the dorsal midline, the receptors for WGA were reduced to about half. After this period, the two lectin receptors did not show significant changes. This result suggests that sugar residues or the sugar-chain skeleton on basal plate cells are altered during neurulation.
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References
Allen AK, Neuberger A, Sharon N (1973) The purification, composition and specificity of wheat-germ agglutinin. Biochem J 131:155–162
Avrameas S (1969) Coupling of enzymes to proteins with glutaraldehyde. Use of the conjugates for the detection of antigens and antibodies. Immunochemistry 6:43–52
Bancroft M, Bellairs R (1974) The onset of differentiation in the epiblast of the chick blastoderm (SEM and TEM). Cell Tissue Res 155:399–418
Brabec RK, Peters BP, Bernstein IA, Gray RH, Goldstein IJ (1980) Differential lectin binding to cellular membranes in the epidermis of the newborn rat. Proc Natl Acad Sci USA 77:477–479
Burger MM, Goldberg AR (1967) Identification of a tumor-specific determinant on neoplastic cell surfaces. Proc Natl Acad Sci USA 57:359–366
Cook GMW, Zalik SE, Milos N, Scott V (1979) A lectin which binds specifically to β-d-galactoside groups is present at the earliest stages of chick embryo development. J Cell Sci 38:293–304
Currie JR, Pfenninger KH, Maylié-Pfenninger M-F (1978) Cell-surface saccharides in developing neuroectoderm. J Cell Biol 79:56A
Currie JR, Maylié-Pfenninger M-F, Pfenninger KH (1984) Developmentally regulated plasmalemmal glycoconjugates of the surface and neural ectoderm. Dev Biol 106:109–120
Fukuda M, Fukuda MN, Hakomori, S-I (1979) Developmental change and genetic defect in the carbohydrate structure of Band 3 glycoprotein of human erythrocyte membrane. J Biol Chem 254:3700–3703
Goldstein IJ, Hayes CE (1978) The lectins: Carbohydrate-binding proteins of plants and animals. Adv Carbohydr Chem Biochem 35:127–340
Hamburger V, Hamilton HL (1951) A series of normal stages in the development of the chick embryo. J Morphol 88:49–92
Hook SL, Sanders EJ (1977) Concanavalin A-binding by cells of the early chick embryo. J Cell Physiol 93:57–68
Inbar M, Huet C, Oseroff AR, Benbassat H, Sachs L (1973) Inhibition of lectin agglutinability by fixation of the cell surface membrane. Biochim Biophys Acta 311:594–599
Kawai N, Nishiyama F, Hirano H (1979) Changes of lectin-binding sites on the embryonic muscle cell surface in the developing ascidian,Halocynthia aurantium. Exp Cell Res 122:293–304
Kleinschuster SJ, Moscona AA (1972) Interactions of embryonic and fetal neural retina cells with carbohydrate-binding phytoagglutinins: Cell surface changes with differentiation. Exp Cell Res 70:397–410
Krach SW, Green A, Nicolson GL, Oppenheimer SB (1974) Cell surface changes occurring during sea urchin embryonic development monitored by quantitative agglutination with plant lectins. Exp Cell Res 84:191–198
Luft JH (1961) Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol 9:409–414
Moscona AA (1971) Embryonic and neoplastic cell surfaces: availability of receptors for concanavalin A and wheat germ agglutinin. Science 171:905–907
Muramatsu T, Gachelin G, Damonneville M, Delarbre C, Jacob F (1979) Cell surface carbohydrates of embryonal carcinoma cells: polysaccharidic side chains of F9 antigens and of receptors to two lectins, FBP and PNA. Cell 18:183–191
Nicolson GL, Singer SJ (1971) Ferritin-conjugated plant agglutinins as specific saccharides stains for electron microscopy: application to saccharides bound to cell membranes. Proc Natl Acad Sci USA 68:942–945
Nicolson GL, Singer SJ (1974) The distribution and asymmetry of mammalian cell surface saccharides utilizing ferritin conjugated plant agglutinins as specific saccharide stains. J Cell Biol 60:236–248
Nicolson GL, Blaustein J, Etzler ME (1974) Characterization of two plant lectins fromRicinus communis and their quantitative interaction with a murine lymphoma. Biochemistry 13:196–204
O'Dell DS, Tencer R, Monroy A, Brachet J (1974) The pattern of concanavalin A-binding sites during the early development ofXenopus laevis. Cell Differ 3:193–198
Pfenninger KH, Maylié-Pfenninger M-F (1979) Properties and dynamics of plasmalemmal glycoconjugates in growing neurites. Prog Brain Res 51:83–94
Reano A, Faure M, Jacques Y, Reichert U, Schaefer H, Thivolet J (1982) Lectins as markers of human epidermal cell differentiation. Differentiation 22:205–210
Rosenblith JZ, Ukena TE, Yin HH, Berlin RD, Karnovsky MJ (1973) A comparative evaluation of the distribution of concanavalin A-binding sites on the surfaces of normal, virally-transformed, and protease-treated fibroblasts. Proc Natl Acad Sci USA 70:1625–1629
Sanders EJ, Anderson AR (1979) Ultrastructural localization of wheat germ agglutinin-binding sites on surfaces of chick embryo cells during early differentiation. J Cell Physiol 99:107–124
Sato T (1967) A modified method for lead staining of thin sections. J Electron Microsc 16:193
Sieber-Blum M, Cohen AM (1978) Lectin binding to neural crest cells. Changes of the cell surface during differentiation in vitro. J Cell Biol 76:628–638
Takahashi H, Howes RI, Anglin JR (1979) Binding pattern of some fluorescent labeled lectins during neural tube closure in the chick embryo. Anat Rec 193:758
van Wauwe JP, Loontiens FG, de Bruyne CK (1973) The interaction ofRicinus communis hemagglutinin with polysaccharides and low molecular weight carbohydrates. Biochim Biophys Acta 313:99–105
Watanabe M, Takeda Z, Urano Y, Muramatsu T (1982) Lectins as reagents to detect differentiation-dependent alterations of carbohydrates. In: Muramatsu T, Ikeda Y (eds). Teratocarcinoma and cell surface. First Editions, Japan Scientific Societies Press, Tokyo, Academic Press, Tokyo, pp 217–228
Zalik SE, Cook GMW (1976) Comparison of early embryonic and differentiating cell surfaces. Interaction of lectins with plasma membrane components. Biochim Biophys Acta 419:119–136
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Takahashi, H., Howes, R.I. Binding pattern of ferritin-labeled lectins (RCAI and WGA) during neural tube closure in the bantam embryo. Anat Embryol 174, 283–288 (1986). https://doi.org/10.1007/BF00698778
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DOI: https://doi.org/10.1007/BF00698778