Summary
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1.
A circadian rhythm in the frequency and amplitude of compound action potential (CAP) from the isolated eye ofAplysia persists for a week or morein vitro in constant darkness. This rhythm has a period of about 26 hours (Figs. 1,2) in a specific culture medium and may express several periodic amplitude components (Fig. 3).
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2.
Constant light (LL) of low intensity shortens the period (Pig. 4) and reduces the range of oscillations. Higher intensity LL results in a further reduction in range, a greater variability of CAP frequency from hour to hour, alterations in the period, and possibly rhythm splits (Fig. 5).
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3.
Pulses of light given at specific points in the circadian cycle shift the phase of the rhythm (Fig. 7). The resulting phase response curve (Fig. 8) is similar to response curves for the activity of diurnal animals and potassium pulses on the eye rhythm.
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I thank Heb Soon Kim for technical assistance and Arthur Winfree for comments on a preliminary draft. Supported by NIH grant 08443.
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Jacklet, J.W. The effects of constant light and light pulses on the circadian rhythm in the eye ofAplysia . J. Comp. Physiol. 90, 33–45 (1974). https://doi.org/10.1007/BF00698365
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DOI: https://doi.org/10.1007/BF00698365