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Electron microscopical demonstration of nucleic acids in virus-like particles in the skeletal muscle of a traffic accident victim

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Summary

Picornavirus-like crystalline arrays were found in the skeletal muscle of a victim of a traffic accident. Individual particles measured approximately 22 nm in diameter and were arrayed in a hexagonal or triangular pattern. Cis-platinum (II) staining of these virus-like particles showed that these particles were not glycogen but contained nucleic acids, and the staining according to Bernhard also showed these particles contained RNA. Through the combination of the methods of Heinen (1977) and Bernahrd (1969), we can confirm that virus-like crystalline structures found electron microscopically contain nucleic acids and possibly represent virions.

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References

  • Aggarwal, S. K., Wagner, R. W., McAllister, P. K., Rosenberg, B.: Cell-surface-associated nucleic acid in tumorigenic cells made visible with platinum-pyrimidine complexes by electron microscopy. Proc. Natl. Acad. Sci. USA72, 928–932 (1975)

    Google Scholar 

  • Alvira, M. M., Mendoza, M.: Reye's syndrome: a viral myopathy? N. Engl. J. Med.292, 1297 (1975)

    Google Scholar 

  • Ben-Bassat, M., Machtey, I.: Picornavirus-like structures in acute dermatomyositis. Am. J. Clin. Pathol.58, 245–249 (1972)

    Google Scholar 

  • Bernhard, W.: A new staining procedure for electron microscopical cytology. J. Ultrastruct. Res.27, 250–265 (1969)

    Google Scholar 

  • Burch, G. E., Sohel, R. S., Colcolough, H. L.: Virus-like particles in skeletal muscle of a heat stroke victim. Arch. Environ. Health17, 984–985 (1968)

    Google Scholar 

  • Byers, B.: Ribosome cyrstallization induced in chick embryo tissues by hypothermia. J. Cell Biol.30, C1-C6 (1966)

    Google Scholar 

  • Caulfield, J. B., Rebeiz, J., Adams, R. D.: Viral involvement of human muscle. J. Path. Bact.96, 232–234 (1968)

    Google Scholar 

  • Chou, S. M., Gutmann, L.: Picornavirus-like crystals in subacute polymyositis. Neurology (Minneap.)20, 205–213 (1970)

    Google Scholar 

  • Chou, S. M.: Prospects of viral etiology in polymyositis. In: Clinical studies in myology (ed. B. A. Kakulas, pp. 17–28. Amsterdam: Excerpta Medica 1971

    Google Scholar 

  • Culebras, A., Merk, F. B.: Cytoplasmic inclusion bodies in superior rectus muscle of the eye. Neurology (Minneap.)25, 422–429 (1975)

    Google Scholar 

  • De Reuck, J., De Coster, W., Inderadjaja, N.: Acute viral polymyositis with predominant diaphragm involvement. J. Neurol. Sci.33, 453–460 (1977)

    Google Scholar 

  • Dobowitz, V., Brooke, M. H.: Muscle biopsy: A modern approach. London, Philadelphia, Toronto: Saunders (1977)

    Google Scholar 

  • Fukuyama, Y., Ando, T., Yokota, J.: Acute fulminant myoglobinuric polymyositis with picornavirus-like crystals. J. Neurol. Neurosurg. Psychiatry40, 775–781 (1977)

    Google Scholar 

  • Hanson, P. A., Urizar, R. E.: Reye's syndrome — virus or artefact in muscle? N. Engl. J. Med.293, 505–506 (1975)

    Google Scholar 

  • Heinen, E.: Cis-dichloro-diammine platinum (II) as stain for electron microscopic preparations. Histochemistry51, 257–260 (1977)

    Google Scholar 

  • Mastaglia, F. L., Walton, J. N.: Coxsackie virus-like particles in skeletal muscle from a case of polymyositis. J. Neurol. Sci.11, 593–599 (1970)

    Google Scholar 

  • Melnick, J. L., Cockburn, W. C., Dalldorf, G., Gard, S., Gear, J. H. McHammon, D. W., Kaplan, M. M., Nagler, F. P., Oker-Blom, N., Rhodes, A. J., Sabin, A. B., Verlinde, J. D., Manus, H. von: Picorna virus group. Virology19, 114–116 (1963)

    Google Scholar 

  • Millard, M. M., Macquet, J. P., Theophanides, T.: X-ray photoelectron spectroscopy of DNA·Pt complexes. Evidence of O6(Gua)·N7(Gua) chelation of DNA with cis-dichlorodiammine platinum (II). Biochim. Biophys. Acta402, 166–170 (1975)

    Google Scholar 

  • Norris, F. H., Jr., Dramov, B., Calder, C. D., Johnson, S. G.: Virus-like particles in myositis accompanying herpes zoster. Arch. Neurol21, 25–31 (1969)

    Google Scholar 

  • Oshiro, L. S., Cremer, N. E., Norris, F. H., Jr., Lennette, E. H.: Virus-like paticles in muscle from a patient with amyotrophic lateral sclerosis. Neurology (Minneap.)26, 57–60 (1976)

    Google Scholar 

  • Sato, T.: Viral myositis. Naika (Tokyo)28, 407–411 (1977) (Japanese)

    Google Scholar 

  • Schiller, H. H., Mair, W. G. P.: Ultrastructural changes of muscle in malignant hyperthermia. J. Neurol. Sci.21, 93–100 (1974)

    Google Scholar 

  • Schiller, H. H.: Chronic viral myopathy and malignant hyperthermia. N. Engl. J. Med.292, 1409 (1975)

    Google Scholar 

  • Schmalbruch, H.: Kristalloide in menschlichen Sklelettmuskelfasern Naturwissenschaften54, 519 (1967)

    Google Scholar 

  • Tang, T. T., Sedmak, G. V., Siegesmund, K. A., McCreadie, S. R.: Chronic myopathy associated with coxsackievirus type A9. A combined electron microscopical and viral isolation study. N. Engl. J. Med.292, 608–611 (1975)

    Google Scholar 

  • Tomonaga, M., Izumiyama, N., Takebe, S.: Electron microscopic observations of the inclusion bodies in ocular muscle of the aged. J. Clin. Electron Microscopy11, 327–336 (1978)

    Google Scholar 

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Fukuhara, N. Electron microscopical demonstration of nucleic acids in virus-like particles in the skeletal muscle of a traffic accident victim. Acta Neuropathol 47, 55–59 (1979). https://doi.org/10.1007/BF00698273

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  • DOI: https://doi.org/10.1007/BF00698273

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