Skip to main content
SpringerLink
Log in

Eigenschaften von isolierten Mitochondrien des Aales und ihre Abhängigkeit von der Adaptationstemperatur unter besonderer Berücksichtigung der oxidativen Phosphorylierung

Properties of isolated mitochondria of the eel and their dependency on acclimation temperature with special regard to oxidative phosphorylation

  • Published:
Journal of comparative physiology Aims and scope Submit manuscript

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Abbreviations

AT:

Adaptationstemperatur

α-KG:

α-Ketoglutarat

Asc:

Ascorbat

β-OH-but:

β-Hydroxybutyrat

Ch.-P.-Med.:

Chappel-Perry-Medium

DNP:

Dinitrophenol

EDTA:

Äthylendiamintetraacetat

FFA(s):

freie Fettsäure(n)

Glu:

Glutamat

LM:

Lebermitoohondrien

Mal:

Malat

MM:

Mitochondrion aus roter Muskulatur

MST-Med.:

Mannit-Saccharose-Tris-Medium

MSTE-Med.:

Mannit-Saccharose-Tris-EDTA-Medium

MSTEA-Med.:

Mannit-Saccharose-Tris-EDTA-Albumin-Medium

n:

Anzahl der Messungen

Pa :

anorganisches Phosphat

Prot:

Protein

PVP:

Polyvinylpyrrolidon

RK:

Respirationskontrolle

rot:

Rotenon

RR:

Respirationsrate

Sed:

Sediment

Succ:

Succinat

TMPD:

Tetramethyl-p-Phenylendiamin

Tris:

Trishydroxymethyl-aminomethan

Üb:

Überstand

VT:

Versuchstemperatur

y:

Anzahl der Tiere

z:

Anzahl der Präparationen

Literatur

  • Adam, H.: Adenosin-5′-diphosphat und Adenosin-5′-monophosphat. In: Bergmeyer, H. U., Hrsg. Methoden der enzymatischen Analyse, S. 573–577. Weinheim/Bergstraße: Verlag Chemie 1962

    Google Scholar 

  • Appelmans, E., Wattiaux, R., De Duve, C.: Tissue fractionation studies. 5. The association of acid phosphatase with a special class of cytoplasmatic granules in rat liver. Biochem. J.59, 438–445 (1955)

    Google Scholar 

  • Beisenherz, G., Boltze, H. J., Bücher, Th., Czok, K., Carbade, K. H., Meyer-Arendt, E., Pfleiderer, G.: Diphosphofructose-Aldolase, Phosphoglyceraldehyd-Dehydrogenase, Milchsaure-Dehydrogenase, Glycerophosphat-Dehydrogenase und Pyruvatkinase aus Kaninchenmuskulatur in einem Arbeitsgang. Z. Naturforsch. (B)8, 555–577 (1953)

    Google Scholar 

  • Bergmeyer, H. U.: Lactat-Dehydrogenase. In: Bergmeyer, H. U., Hrsg., Methoden der enzymatischen Analyse, S. 736–740. Weinheim/Bergstraße: Verlag Chemie 1962

    Google Scholar 

  • Bertin, L.: Eels, a biological study. London: Cleaver-Hume 1956

    Google Scholar 

  • Bhuvaneswaran, C., Dakshinamurti, K.: Energy-linked swelling of biotin-deficient rat liver mitochondria. Arch. Biochem. Biophys.142, 665–674 (1971)

    Google Scholar 

  • Borst, P., Loos, J. A., Christ, E. J., Slater, E.C.: Uncoupling activity of longchain fatty acids. Biochim. biophys. Acta (Amst.)62, 509–518 (1962)

    Google Scholar 

  • Brdiczka, D., Pette, D.: Intra- and extramitochondrial isozymes of (NADP) malate dehydrogenase. Europ. J. Biochem.19, 546–551 (1971)

    Google Scholar 

  • Brockerhoff, H.: Fatty acid distribution patterns of animal depot fats. Comp. Biochem. Physiol.19, 1–12 (1966)

    Google Scholar 

  • Caldwell, R. S.: Temperature acclimation in the goldfish (Carassius awratus L.): Studies on terminal electron transport and the role of lipids. Ph. D. Thesis, Duke University, North Carolina, 1967

    Google Scholar 

  • Caldwell, R. S.: Thermal compensation of respiratory enzymes in tissues of the goldfish (Carrassius auratus L.). Comp. Biochem. Physiol.31, 79–93 (1969)

    Google Scholar 

  • Caldwell, R. S., Vernberg, F. J.: The influence of acclimation temperature on the lipid composition of fish gill mitochondria. Comp. Biochem. Physiol.34, 179–191 (1970)

    Google Scholar 

  • Chambell, R. C.: Statistische Methoden für Biologie und Medizin. Stuttgart: Thieme 1971

    Google Scholar 

  • Chance, B.: The reactivity of haemoproteins and cytochromes. Biochem. J.103, 1–18 (1967)

    Google Scholar 

  • Chance, B., Hagihara, B.: Direct spectroscopic measurements of interaction of components of the respiratory chain with ATP, ADP, phosphate and uncoupling agents. In: Slater, E. C., ed.: Sym. Intracellular Respiration, Proc. 5th Intern. Congr. Biochem., Moscow, 1961, vol. 5, p. 3–37. London-New York: Pergamon Press 1963

    Google Scholar 

  • Chance, B., Lee, C. P., Mela, L., De Vault, D.: Mechanism and control of electron transfer. In: Okunuki, K., Kamen, M. D., Sekuzu, I., eds., Structure and function of cytochromes, p. 475–487. Tokyo: University of Tokyo Press; Baltimore, Maryland-Manchester, England: University Park Press 1968

    Google Scholar 

  • Chance, B., Williams, G. R.: The respiratory chain and oxidative phosphorylation. Advanc. Enzymol.17, 65–134 (1956)

    Google Scholar 

  • Chang, S. B., Matson, R. S.: Membrane stability (thermal) and nature of fatty acids in yeast cells. Biochem. biophys. Res. Commun.46, 1529–1535 (1972)

    Google Scholar 

  • Changeux, J. P., Thiery, J., Tung, Y., Kittel, C.: On the cooperativity of biological membranes. Proc. nat. Acad. Sci. (Wash.)57, 335–341 (1967)

    Google Scholar 

  • Chappel, J. B., Perry, S. V.: Biochemical and osmotic properties of skeletal muscle mitochondria. Nature (Lond.)173, 1094–1095 (1954)

    Google Scholar 

  • Colowick, S. P., Kaplan, N. O.: Methods in enzymology, vol. X, Oxidation and phosphorylation. New York-London: Academic Press 1967

    Google Scholar 

  • Dakshinamurti, K., Sabir, M. A., Bhuvaneswaran, C.: Oxidative phosphorylation by biotin-deficient rat liver mitochondria. Arch. Biochem. Biophys.137, 30–37 (1970)

    Google Scholar 

  • Davis, J. T.: A technique for the isolation of mitochondria from bovine lymphocytes. In: Colowick, S. P., Kaplan, N. O., eds., Methods in enzymology, vol. X, Oxidation and phosphorylation, p. 114–117. New York-London: Academic Press 1967

    Google Scholar 

  • Deenen, L. L. M. van: In: 62nd annual Meeting American Society of Biological Chemists, San Francisco, California, 1971 (zit. nach Chang und Matson, 1972)

  • De Jong, J. W.: Influence of adenosine and nagarse on palmitoyl-CoA synthetase in rat heart and liver mitochondria. Biochim. biophys. Acta (Amst.)245, 288–298 (1971)

    Google Scholar 

  • De Jong, J. W., Hülsmann, W. C.: A comparative study of palmitoyl-CoA synthetase activity in rat liver, heart and gut mitochondrial and microsomal preparations. Biochim. biophys. Acta (Amst.)197, 127–135 (1970)

    Google Scholar 

  • Estabrook, R. W.: Mitochondrial respiratory control and the polarographic measurement of ADP: 0 ratios. In: Colowick, S. P., Kaplan, N. 0., eds., Methods in enzymology, vol. X, Oxidation and phosphorylation, p. 41–47. New York-London: Academic Press 1967

    Google Scholar 

  • Freed, J.: Changes in activity of cytochrome oxidase during adaptation of goldfish to different temperatures. Comp. Biochem. Physiol.14, 651–659 (1965)

    Google Scholar 

  • Frehn, J. L., Anthony, A.: Respiration and phosphorylation of liver mitochondria from cold-exposed rats and chipmunks. Amer. J. Physiol.203, 821–824 (1962)

    Google Scholar 

  • Gumbmann, M., Tappel, A. L.: The tricarboxylic acid cycle in fish. Arch. Biochem. Biophys.98, 262–270 (1962)

    Google Scholar 

  • Hackenbrock, Ch. R.: Ultrastructural base for metabolically linked mechanical activity in mitochondria I. Reversible ultrastructural changes with change in metabolic steady state in isolated liver mitochondria. J. Cell. Biol.30, 269–297 (1966)

    Google Scholar 

  • Hagihara, B.: Technique for the application of polarography to mitochondrial respiratory. Biochim. biophys. Acta (Amst.)46, 134–142 (1961)

    Google Scholar 

  • Hazel, J. R.: The effect of temperature acclimation upon succinic dehydrogenase activity from the epaxial muscle of the common goldfish (Carassius auratue L.) — I. Properties of the enzyme and the effect of lipid extraction. Comp. Biochem. Physiol.43 B, 837–861 (1972)

    Google Scholar 

  • Hazel, J. R.: The effect of temperature acclimation upon succinic dehydrogenase activity from the epaxial muscle of the common goldfish (Carassius auratus L.) — II. Lipid reactivation of the soluble enzyme. Comp. Biochem. Physiol.43 B, 862–882 (1972)

    Google Scholar 

  • Hoch, F.: Energy transformations in animals: regulatory mechanisms. Philadelphia-London-Toronto: W. B. Saunders Co. 1971

    Google Scholar 

  • Hogeboom, G. H., Schneider, W. C., Palade, G. E.: Cytochemical studies of mammalian tissues — I. Isolation of intact mitochondria from rat liver; some biochemical properties of mitochondria and submitochondrial particulate material. J. biol. Chem.172, 619–635 (1948)

    Google Scholar 

  • Jankowsky, H.-D.: Versuche zur Adaptation der Fische im normalen Temperaturbereich. Helgoländer wiss. Meeresunters.18, 317–362 (1968)

    Google Scholar 

  • Kadenbach, B.: Der Einfluß von Thyreoidhormonen in vivo auf die oxidative Phosphorylierung und Enzymaktivitäten in Mitochondrion. Biochem. Z.344, 49–75 (1966)

    Google Scholar 

  • Kanungo, M. S., Prosser, C. L.: Physiological and biochemical adaptation of goldfish to cold and warm temperatures — I. Standard and active oxygen consumptions of cold — and warm — acclimated goldfish at various temperatures. J. cell. comp. Physiol.54, 265–274 (1959a)

    Google Scholar 

  • Kanungo, M. S., Prosser, C. L.: Physiological and biochemical adaptation of goldfish to cold and warm temperatures — II. Oxygen consumption and oxidative phosphorylation of liver mitochondria. J. cell. comp. Physiol.54, 265–274 (1959b)

    Google Scholar 

  • Kleinschmidt, M. G., McMahon, V. A.: Effect of growth temperature on the lipid composition of cyanidium caldarium. I. Class seperation of lipids. Plant Physiol.46, 286–289 (1970)

    Google Scholar 

  • Klingenberg, M.: Reduziertes Diphospho-pyridinnucleotid. In: Bergmeyer, H. U., Hrsg., Methoden der enzymatischen Analyse, S. 531–534. Weinheim/Bergstraße: Verlag Chemie 1962

    Google Scholar 

  • Klingenberg, M.: Muskelmitochondrien. In: Ergebnisse der Physiologie, Bd. 55, S. 131–189. Berlin-Göttingen-Heidelberg-New York: Springer 1964

    Google Scholar 

  • Korman, E. F., De Pury, G., Asai, J., Allmann, D. W., Kopaczky, K., Green, D. E.: On the structure arising from the interaction of phospholipid micelles with headpiece-stalk sectors detached from the mitochondrial cristal membrane. Biochemistry9, 1318–1326 (1970)

    Google Scholar 

  • Lardy, H. A., Johnson, D., McMurray, W. C.: A survey of toxic antibiotics in respiratory, phosphorylative, and glycolytic systems. Arch. Biochem. Biophys.78, 587–597 (1958)

    Google Scholar 

  • Lee, C.-P., Ernster, L.: Relationship between energy-linked nicotinamide nucleotide transhydrogenation and oxidative phosphorylation. In: Quagliariello, E., Papa, S., Slater, E. C., Tager, J. M., eds., Mitochondrial structure and compartimentation, p. 353–362. Bari: Adriatica editrice 1967

    Google Scholar 

  • Linhard, K., Walter, K.: Phosphatasen (Phosphomonoesterasen). In: Bergmeyer, H.-U., Hrsg., Methoden der enzymatischen Analyse, S. 779–782. Weinheim/Bergstraße: Verlag Chemie 1962

    Google Scholar 

  • Linnane, A. W., Haslam, J. M., Forrester, I. T.: The influence of altered membrane lipid composition on mitochondrial nucleic acid synthesis and oxidative phosphorylation inSaccharomyces cerevisae. In: Azzone, G. F., Carafoli, E., Lehninger, A. L., Quagliariello, E., Siliprandi, N., eds., Biochemistry and biophysics of mitochondrial membranes, p. 523–539. New York-London: Academic Press 1972

    Google Scholar 

  • Lyons, J. M., Raison, J. K.: A temperature-induced transition in mitochondrial oxidation: Contrasts between cold and warm-blooded animals. Comp. Biochem. Physiol.37, 405–411 (1970)

    Google Scholar 

  • Mahler, H. R., Cordes, E. H.: Biological chemistry. New York-Evanston-London: Harper and Row 1969

    Google Scholar 

  • Malessa, P.: Beiträge zur Temperaturadaptation des Aales (Anguilla vulgaris) III. Höhe und Verteilung der Aktivität von Succinodehydrogenase und Cytocnrom-oxidase im Seitenmuskel juveniler und adulter Tiere. Mar. Biol.3, 143–158 (1969)

    Google Scholar 

  • Martius, C., Hess, B.: The mode of action of thyroxine. Arch. Biochem.33, 486–487 (1951)

    Google Scholar 

  • Massey, B. D., Smith, C. L.: The action of thyroxine on mitochondrial respiration and phosphorylation in the trout (Salmo trutta fario L.). Comp. Biochem. Physiol.25, 241–255 (1968)

    Google Scholar 

  • Mayerle, J. A., Butler, D. G.: Effects of temperature and feeding on intermediary metabolism in North American eels (Anguilla rostrata Lesueur). Comp. Biochem. Physiol.40 A, 1087–1095 (1971)

    Google Scholar 

  • Mellors, A., Tappel, A. L., Sawant, P. L., Desai, I. D.: Mitochondrial swelling and uncoupling of oxidative phosphorylation by lysosomes. Biochim. biophys. Acta (Amst.)143, 299–309 (1967)

    Google Scholar 

  • Mitchell, P.: Coupling of phosphorylation to electron and hydrogen transfer by a chemi-osmotic typ of mechanism. Nature (Lond.)191, 144–148 (1961)

    Google Scholar 

  • Nichols, D. G., Garland, P. B.: The control of isocitrate oxidation by rat liver mitochondria. Biochem. J.114, 215–225 (1969)

    Google Scholar 

  • Pande, S.V., Blanchaer, M. C.: Preferential loss of ATP-dependent long-chain fatty acid activating enzyme in mitochondria prepared using nagarse. Biochim. biophys. Acta (Amst.)202, 43–48 (1970)

    Google Scholar 

  • Papa, S., Francavilla, A.: Enzyme compartimentation and hydrogen transfer reactions of the level of nicotinamide nucleotides in rat liver mitochondria. In: Quagliariello, E., Papa, S., Slater, E. C., Tager, J. M., eds., Mitochondrial structure and compartimentation, p. 363–372. Bari: Adriatica editrice 1967

    Google Scholar 

  • Precht, H.: Der Einfluß der Temperatur auf die Atmung und auf einige Fermente beim Aal (Anguilla vulgaris L.). Biol. Zbl.70, 71–85 (1951)

    Google Scholar 

  • Precht, H.: Beiträge zur Temperaturadaptation des Aales (Anguilla vulgaris L.) I. Z. vergl. Physiol.44, 451–462 (1961)

    Google Scholar 

  • Precht, H. (editor): Temperature and life, 2nd ed. Berlin-Heidelberg-New York: Springer 1973, in press

    Google Scholar 

  • Richardson, T., Tappel, A. L.: Swelling of fish mitochondria. J. Cell Biol.13, 43–53 (1962)

    Google Scholar 

  • Richardson, T., Tappel, A. L., Gruger, Jr., E. H.: Essential fatty acids in mitochondria. Arch. Biochem. Biophys.94, 1–6 (1961)

    Google Scholar 

  • Richardson, T., Tappel, A. L., Smith, L. M., Houle, C. R.: Polyunsaturated fatty acids in mitochondria. J. Lipid Res.3, 344–350 (1962)

    Google Scholar 

  • Sanadi, D. R., Jacobs, E. E.: Assay of oxidative phosphorylation at the cytochromoxidase region (site III). In: Colowick, S. P., Kaplan, N. O., eds., Methods in enzymology, vol. X, Oxidation and phosphorylation, p. 38–41. New York-London: Academic Press 1967

    Google Scholar 

  • Scheil, H. G.: Beiträge zur Temperaturadaptation des AalesAnguilla anguilla L. IV. Einfluß der Adaptationstemperatur auf den denervierten Seitenmuskel. Mar. Biol.6, 158–166 (1970)

    Google Scholar 

  • Schultze, D.: Beiträge zur Temperaturadaptation des Aales (Anguilla vulgaris L.) II. Z. wiss. Zool.172, 104–133 (1965)

    Google Scholar 

  • Skilleter, D. N., Kun, E.: Membrane bound lactate dehydrogenase of rat liver mitochondria. Fed. Proc.29, 930 (Abstr.), No 3899

  • Smith, C. L.: The temperature dependence of oxidative phosphorylation and of the activity of various enzyme systems in liver mitochondria from cold- and warmblooded animals. Comp. Biochem. Physiol.46 B, 445–461 (1973)

    Google Scholar 

  • Smith, R. E.: Mitochondrial control of oxidative phosphorylation in cold-acclimated rats. Fed. Proc.19, 146–151 (1960)

    Google Scholar 

  • Smith, R. E., Fairhurst, A. S.: A mechanism of cellular thermogenesis in cold-adaptation. Proc. nat. Acad. Sci. (Wash.)44, 705–711 (1958)

    Google Scholar 

  • Storey, B. T.: Chemical hypothesis for energy conservation in the mitochondrial respiratory chain. J. theor. Biol.28, 233–259 (1970)

    Google Scholar 

  • Storey, B. T.: Chemical hypothesis for energy conservation in the mitochondrial respiratory chain. II. Redox properties of the coupling site enzymes at sites I and II. J. theor. Biol.31, 533–552 (1971)

    Google Scholar 

  • Szarkowska, L., Klingenberg, M.: On the role of ubiquinone in mitochondria. Biochem. Z.338, 674–697 (1963)

    Google Scholar 

  • Van den Bergh, G. S.: Fatty acid activation and oxidation by mitochondria. In: Colowick, S. P., Kaplan, N. O., eds. Methods in enzymology, vol. X, Oxidation and phosphorylation, p. 749–755. New York-London: Academic Press 1967

    Google Scholar 

  • Waite, M., Deenen, L. L. M. van: Hydrolysis of phospholipids and glycerides byrat liver preparations. Biochim. biophys. Acta (Amst.)137, 498–517 (1967)

    Google Scholar 

  • Wilson, D. E., Dutton, P. L.: The oxidation-reduction potentials of cytochromes a and a3 in intact rat liver mitochondria. Arch. Biochem. Biophys.136, 583–584 (1970)

    Google Scholar 

  • Wilson, D. E., Erecinska, M., Brocklehurst, E. S.: The chemical properties of cytochrome c oxidase in intact mitochondria. Arch. Biochem. Biophys.151, 180–187 (1972)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Zoologisches Institut der Universität Kiel, Deutschland

    Ekkehart Wodtke

Authors
  1. Ekkehart Wodtke
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Teile dieser Arbeit wurden referiert auf dem Symposium „Effects of temperature on heterothermic organisms; ecology and mechanisms” in Obergurgl, Österreich, im September 1972.

Herrn Professor Dr. H.-D. Jankowsky sei für die Anregung zu dieser Arbeit, die Teil meiner Dissertation ist, wie für manchen Eat bei der Abfassung des Manuskriptes gedankt.

Herrn Gerhard Merker und Dr. Inge Vomfelde danke ich für die Einbettung bzw. für das Sehneiden und Photographieren von Mitochondrienpräparationen.

Die Untersuchungen wurden durch die Deutsche Forschungsgemeinschaft unterstützt.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wodtke, E. Eigenschaften von isolierten Mitochondrien des Aales und ihre Abhängigkeit von der Adaptationstemperatur unter besonderer Berücksichtigung der oxidativen Phosphorylierung. J. Comp. Physiol. 91, 277–307 (1974). https://doi.org/10.1007/BF00698059

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00698059

Search

Navigation