Abstract
Ia antigens in rats are genetically associated with typical MHC-linked immune response (Ir) genes. One ratIr gene (IR-GLT) has recently been mapped to theRT1.D locus in a rat MHC recombinant, WRC. We have studied the expression of la antigenic determinants in WRC and its parental strains BN and WRA using a panel of monoclonal antibodies. Our results suggest that the inheritance of1R-GLTcorresponds with the inheritance of I-E-like, but not I-A-like, antigens in the WRC rat. These observations were confirmed when WRC I-E-like antigens were analyzed by two-dimensional gel electrophoresis. We propose thatRT1.D shares both functional and antigenic homologies with the mouseI-E subregion.
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References
Benacerraf, B. and Dorf, M. E.: Genetic control of specific immune responses and immune suppressions by I-region genes.Cold Spring Harbor Symp. Quant. Biol. 41: 465–475, 1976
Bevan, M. J.: The major histocompatibility complex determines susceptibility to cytotoxic T cells directed against minor histocompatibility antigens.J. Exp. Med. 142: 1349–1364, 1975
Blankenhorn, E. P., Cecka, J. M., Götze, D., and Hood, L.: Partial N-terminal amino acid sequence of rat transplantation antigens.Nature 274: 90–92, 1978
Blankenhorn, E. P., Cecka, J. M., Frelinger, J., Götze, D., and Hood, L.: Structure of Ia antigens from the rat. Mouse alloantisera demonstrate at least two distinct molecular species.Eur. J. Immunol. 10: 145–151, 1980
Blankenhorn, E. P., Symington, F. W., Pickard, A., and McKearn, T. J.: Monoclonal antibodies which distinguish rat Ia antigens.Transplant. Proc., in press, 1983
Butcher, G. W. and Howard, J. C.: A recombinant in the major histocompatibility complex of the rat.Nature 266: 362–364, 1977
Cecka, J. M., Blankenhorn, E. P., Götze, D., and Hood, L.: Microsequence analysis of Ia antigens from three strains of rats.Eur. J. Immunol. 10: 140–145, 1980
Doherty, P. C. and Zinkernagel, R. M.: A biological role for major histocompatibility antigens.Lancet 1: 1406–1409, 1975
Dorf, M. E. (ed.): Role of the Major Histocompatibility Complex in Immunobiology. Garland Press, New York, 1981
Frelinger, J. G., Hood, L., and Wettstein, P.: Analyses of RT1 products using two-dimensional polyacrylamide gels.Transplant. Proc. 13: 1360–1363, 1981
Gill, T. J., Kunz, H. W., Schaid, D. J., VandeBerg, J. L., and Stole, V.: Orientation of loci in the major histocompatibility complex of the rat and its comparison to man and the mouse.J. Immunogenet., in press, 1983
Götze, D.: The Major Histocompatibility System in Man and Animals, Springer Verlag, New York, 1977
Günther, E. and Štark, O.: The major histocompatibility system of the rat (AgB or H-1).In D. Götze (ed.):The Major Histocompatibility System in Man and Animals, pp. 207–253, Springer Verlag, New York, 1977
Günther, E., Štark, O., and Koch, C.: Genetic definition of I-region-determined antigens of the rat major histocompatibility complex.Eur. J. Immunol. 8: 206–212, 1978
Jones, P. P., Murphy, D. B., and McDevitt, H. O.: Two gene control of the expression of a murine Ia antigen.J. Exp. Med. 148: 925–939, 1978
Klinman, D.: Recognition of class I major histocompatibility antigens. Doctoral thesis, University of Pennsylvania, 1982
Klinman, D. M. and Howard, J. C.: Protein iodination suitable for labeling hybridoma antibodies.In R. H. Kennett, T. J. McKearn, and K. B. Bechtol (eds.):Monoclonal Antibodies, pp. 401–402, Plenum Press, New York, 1980
Kunz, H. W., Gill, T. J. III, and Misra, D. N.: The identifications and mapping of a second class I locus in the major histocompatibility complex of the rat.J. Immunol. 128: 402–408, 1982
Lobel, S. A. and Cramer, D. V.: Demonstration of a new genetic locus in the major histocompatibility system of the rat.Immunogenetics 13: 465–473, 1981
Marshak, A., Doherty, P. C., and Wilson, D. B.: The control of specificity of cytotoxic T lymphocytes by the major histocompatibility complex of rats, and identification of a new alloantigen system showing no AgB restriction.J. Exp. Med. 146: 1773–1790, 1977
McKearn, T. J., Fitch, F. W., Smilek, D. E., Sarmiento, M., and Stuart, F. P.: Properties of rat anti-MHC antibodies produced by cloned rat-mouse hybridomas.Immunol. Rev. 47: 91–115, 1979
McNicholas, J. M., Murphy, D. B., Matis, L. A., Schwartz, R. H., Lerner, E. A., Janeway, C. A., Jr., and Jones, P. P.: Immune response gene function correlates with the expression of an Ia antigen.J. Exp. Med. 155: 490–507, 1982
Milstein, C. and Lennox, E.: The use of monoclonal antibody techniques in the study of developing cell surfaces.Curr. Top. Dev. Biol. 14: 1–32, 1980
Nagy, Z. A., Baxevanis, C. N., Ishii, N., and Klein, J.: Ia antigens as restriction molecules in Ir-gene controlled T-cell proliferation.Immunol. Rev. 60: 59–83, 1981
O'Farrell, P. Z., Goodmann, H. M., and O'Farrell, P. H.: High resolution two dimensional electrophoresis of basic as well as acidic proteins.Cell 12: 1133–1141, 1977
Oi, V. T., Jones, P. P., Goding, J. W., Herzenberg, L. A., and Herzenberg, L. A.: Properties of monoclonal antibodies to mouse Ig allotypes, H-2, and Ia antigens.Curr. Top. Microbiol. Immunol. 81: 115–129, 1978
Ozato, K., Mayer, N., and Sachs, D. H.: Hybridoma cell lines secreting monoclonal antibodies to mouse H-2 and Ia antigens.J. Immunol. 124: 533–541, 1980
Singh, S. K., Wakeland, E. K., Vučak, I., Nagy, Z. A., and Klein, J.: AnH-2 haplotype possibly derived by crossing-over between the (A α B β ) duplex and the E β locus.Immunogenetics 14: 273–281, 1981
Smilek, D. E., Boyd, H., Wilson, D. B., Zmijewski, C. M., Fitch, F. W., and McKearn, T. J.: Monoclonal rat anti-major histocompatibility complex antibodies display specificity for rat, mouse, and human target cells.J. Exp. Med. 151: 1139–1150, 1980
Symington, F. W.: Monoclonal antibodies specific for immune cell subpopulations. Doctoral thesis, University of Pennsylvania, 1982
Symington, F. W. and Sprent, J.: A monoclonal antibody detecting an Ia specificity mapping in the I-A or I-E subregion.Immunogenetics 14: 53–61, 1981
Zinkernagel, R. M., Althage, A., and Jensen, F. C.: Cell-mediated immune response to lymphocytic choriomeningitis and vaccinia virus in rats.J. Immunol. 119:1242–1247, 1977
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Blankenhorn, E.P., Symington, F.W. & Cramer, D.V. Biochemical characterization of Ia antigens encoded by theRT1.B andRT1.D loci in the rat MHC. Immunogenetics 17, 475–484 (1983). https://doi.org/10.1007/BF00696871
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DOI: https://doi.org/10.1007/BF00696871