Summary
Thick sections (0.5–2 μ) of biopsies from human nervous system tumors (Schwannoma, ependymoma, medulloblastoma), fixed in aldehydes followed by osmium, and stained with uranyl acetate and lead, have been studied at 2.5 MV, and compared to thin sections of the same material observed by ordinary low voltage electron microscopy. High voltage electron microscopy permits direct observation of cell fine structure in three dimensions, including the spatial relations of organelles. Details of contours of nuclear envelopes, shapes of mitochondria, fine aspects of the structure of cell surfaces and processes, such as the flat sheet-like and irregular cylindrical processes of Schwannoma cells, the small projections and ridges on their surfaces, and microvilli and cilia of ependymoma cells, and other features have been observed. These initial observations demonstrate the applicability of high voltage electron microscopy to further study of neural neoplasms.
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References
Allen, D. J., Low, F. N.: The ependymal surface of the lateral ventricle of the dog as revealed by scanning electron microscopy. Amer. J. Anat.137, 483–489 (1973)
Bruni, J. E., Montemurro, D. G., Clattenburg, R. E., Singh, R. P.: A scanning electron microscopic study of the ependymal surface of the third ventricle of the rabbit, rat, mouse and human brain. Anat. Rec.174, 407–420 (1972)
Carasso, N., Delaunay, M.-C., Favard, P., Lechaire, J.-P.: Obtention et coloration de coupes epaisses pour la microscopic electronique à haute tension. J. Micr. (Paris)16, 257–268 (1973)
Chan-Palay, V., Palay, S. L.: High voltage electron microscopy of rapid Golgi preparations. Neurons and their processes in the cerebellar cortex of monkey and rat. Z. Anat. Entwickl.-Gesch.137, 125–152 (1972a)
Chan-Palay, V., Palay, S. L.: The form of velate astrocytes in the cerebellar cortex of monkey and rat: High voltage electron microscopy of rapid Golgi preparations. Z. Anat. Entwickl.-Gesch.138, 1–19 (1972b)
Cravioto, H.: The ultrastructure of acoustic nerve tumors. Acta neuropath. (Berl.)12, 116 to 140 (1969)
Dupouy, G.: Performance and applications of the Toulouse 3 million volt electron microscope. J. Micr. (Lond.)97, 3–28 (1973)
Dupouy, G., Perrier, F.: Microscopie électronique à 3 millions de volts. C. R. Acad. Sci. (Paris)274, 1170–1174 (1972)
Dupouy, G., Perrier, F., Durrieu, L.: Microscope électronique trois millions de volts. J. Micr. (Paris)9, 575–592 (1970)
Escourolle, R., Poirier, J.: L'ultrastructure du médulloblastome cérébelleux. Ann. Anat. path.12, 121–136 (1967)
Favard, P., Carasso, N.: The preparation and observation of thick biological sections in the high voltage electron microscope. J. Micr. (Lond.)97, 59–81 (1973)
Favard, P., Ovtracht, L., Carasso, N.: Observations de spécimens biologiques en microscopie électronique à haute tension. I. Coupes épaisses. J. Micr. (Paris)12, 301–316 (1971)
Glauert, A. M., Mayo, C. R.: The study of the three-dimensional structural relationships in connective tissues by high voltage electron microscopy. J. Micr. (Lond.)97, 83–94 (1973)
Goebel, H. H., Cravioto, H.: Ultrastructure of human and experimental ependymomas. J. Neuropath. exp. Neurol.31, 54–71 (1972)
Hama, K., Porter, K. R.: An application of high voltage electron microscopy to the study of biological materials. High voltage electron microscopy. J. Micr. (Paris)8, 149–158 (1969)
Kadin, M. E., Rubinstein, L. J., Nelson, J. S.: Neonatal cerebellar medulloblastoma originating from the fetal external granular layer. J. Neuropath. exp. Neurol.29, 583–600 (1970)
Luse, S. A.: Electronmicroscopic studies of brain tumors. Neurology (Minneap.)10, 881–905 (1960)
Luse, S.: The Schwann cell. In: Pathology of the nervous system, vol. 1, pp. 585–607. J. Minckler (ed.). New York: McGraw Hill Book Co. 1968
Lyser, K. M.: Microtubules and filaments in developing axons and optic stalk cells. Tissue & Cell3, 395–404 (1971)
Lyser, K. M.: Low- and high-voltage electron microscopy of a human neuroblastoma in longterm organ culture. Cancer Res.34, 594–602 (1974)
Lyser, K. M.: Organ cultures of human nervous system tumors. In Vitro (in press 1975)
Matakas, F., Cervós-Navarro, J., Gullotta, F.: The ultrastructure of medulloblastomas. Acta neuropath. (Berl.)16, 271–284 (1970)
Palay, S. L., Chan-Palay, V.: High voltage electron microscopy of the central nervous system in Golgi preparations. J. Micr. (Lond.)97, 41–47 (1973)
Parsons, D. F., Matricardi, V. R., Subjeck, J., Uydess, I., Wray, G.: High-voltage electron microscopy of wet whole cancer and normal cells. Visualization of cytoplasmic structures and surface projections. Biochim. biophys. Acta (Amst.)290, 110–124 (1972)
Poirier, J., Escourolle, R.: Ultrastructure des neurinomes de l'acoustique. Z. mikr.-anat. Forsch.76, 509–525 (1967)
Reynolds, E. S.: The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol.17, 208–212 (1963)
Russell, D. S., Rubinstein, L. J.: Pathology of tumours of the nervous system, 3rd Ed. London: Edw. Arnold Ltd. 1971
Sabatini, D. D., Bensch, K., Barrnett, R. J.: Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation. J. Cell Biol.17, 19–58 (1963)
Scott, D. E., Paull, W. K., Dudley, G. K.: A comparative scanning electron microscopic analysis of the human cerebral ventricular system. 1. The third ventricle. Z. Zellforsch.132, 203–215 (1972)
Tani, E., Ametani, T.: Polygonal crystalline structures in human ependymoma cells. Acta neuropath. (Berl.)15, 359–362 (1970)
Tennyson, V. M., Pappas, G. D.: Ependyma. In: Pathology of the nervous system, vol. 1, pp. 518–531. J. Minckler (ed.). New York: McGraw Hill Book Co. 1968
Tilney, L. G., Porter, K. R.: Studies on microtubules in Helioza. I. The fine structure ofActinosphaerium nucleofilum (Barrett), with particular reference to the axial rod structure. Protoplasma (Wien)60, 317–344 (1965)
Vaughn, J. E., Peters, A.: Electron microscopy of the early postnatal development of fibrous astrocytes. Amer. J. Anat.121, 131–152 (1967)
Voigt, W.-H.: Elektronenmikroskopische Beobachtungen an menschlichen Medulloblastomen. Dtsch. Z. Nervenheilk.192, 290–309 (1968)
Waggener, J. D.: Ultrastructure of benign peripheral nerve sheath tumors. Cancer (Philad.)19, 699–709 (1966)
Wechsler, W., Hossmann, K.-A.: Zur Feinstruktur menschlicher Acusticusneurinome. Beitr. path. Anat.132, 319–343 (1965)
Worthington, W. C., Cathcart, R. S., III: Ependymal cilia: Distribution and activity in the adult human brain. Science139, 221–222 (1963)
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Lyser, K.M. Human nervous system tumors. Acta Neuropathol 32, 313–324 (1975). https://doi.org/10.1007/BF00696793
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DOI: https://doi.org/10.1007/BF00696793