Archives of Microbiology

, Volume 99, Issue 1, pp 255–263 | Cite as

Site of mannan synthesis in yeast

An autoradiographic study
  • A. Košinová
  • V. Farkaš
  • S. Machala
  • Š. Bauer


The combination of high-resolution autoradiography and biochemical methods has been used to ascertain the site of mannan synthesis in the yeastSaccharomyces cerevisiae. High-resolution autoradiography has been performed under conditions when addedd-mannose-3H was incorporated exclusively into mannan. Application of “pulse-chase” labelling technique revealed that the radio-active mannose is fixed primarily in the cytoplasmic space from where it is transported into the cell wall. Additional experiments with separated membrane fractions from the same yeast strongly support the hypothesis that the plasmalemma is not directly involved in the biosynthesis of yeast mannan and that the membranes of the endoplasmic reticulum are the sites where the polymerization of mannosyl units takes place.

Key words

Mannan Synthesis Saccharomyces cerevisiae Autoradiography Cell Wall Yeast 


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Copyright information

© Springer-Verlag 1974

Authors and Affiliations

  • A. Košinová
    • 1
  • V. Farkaš
    • 1
  • S. Machala
    • 1
  • Š. Bauer
    • 1
  1. 1.Institute of ChemistrySlovak Academy of SciencesBratislava

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