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Mannosyl-Oligosaccharide Glucosidase (Glucosidase I, MOGS)

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Handbook of Glycosyltransferases and Related Genes

Abstract

The synthesis of asparagine-linked glycoproteins in eukaryotes occurs via the transfer of dolichol-linked Glc3Man9GlcNAc2 oligosaccharide at specific Asn-X-Ser/Thr sequons via the oligosaccharyl transferase (Kornfeld and Kornfeld 1985; Waechter and Lennarz 1976). Following transfer of this oligosaccharide to the peptide, sugar residues are removed or added by specific enzymes to create a diverse population of high-mannose, hybrid, or complex-type glycans as the glycoprotein moves through the secretory pathway. The first step in the remodeling of the Glc3Man9GlcNAc2 oligosaccharide is the removal of a single α1,2-linked glucose from the nonreducing terminal end of the α1,3Man branch to produce Glc2Man9GlcNAc2 (Fig. 113.1). This initial processing step is completed by mannosyl-oligosaccharide glucosidase (MOGS), also known as α-glucosidase I, which is a member of CAZy glycosyl hydrolase family 63 (Henrissat 1991). The removal of the terminal α1,2-linked glucose in the endoplasmic reticulum is the initial step in the processing and potential remodeling of N-glycans in the secretory pathway. Following removal of the distal α1,2 glucose residue to produce Glc2Man9GlcNAc2, a second ER α-glucosidase, the hetero-dimeric α-glucosidase II, removes the two remaining glucose residues.

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Authors and Affiliations

  1. Complex Carbohydrate Research Center, The University of Georgia, 315 Riverbend Road, Athens, GA, 30602, USA

    Dr. Alison V. Nairn & Prof. Kelley W. Moremen

Authors
  1. Dr. Alison V. Nairn
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  2. Prof. Kelley W. Moremen
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Corresponding author

Correspondence to Alison V. Nairn .

Editor information

Editors and Affiliations

  1. RIKEN-Max-Planck Joint Research Center for Systems Chemical Biology, RIKEN, Wako, Saitama, Japan

    Naoyuki Taniguchi

  2. Department of Biochemistry, Kochi University, Medical School, Nankoku, Kochi, Japan

    Koichi Honke

  3. Sanford Burnham Medical Research Institute, La Jolla, California, USA

    Minoru Fukuda

  4. Research Center for Medical Glycoscience (RCMG), National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan

    Hisashi Narimatsu

  5. RIKEN-Max-Planck Joint Research Center for Systems Chemical Biology, RIKEN, Wako, Saitama, Japan

    Yoshiki Yamaguchi

  6. Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan

    Takashi Angata

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© 2014 Springer Japan

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Nairn, A.V., Moremen, K.W. (2014). Mannosyl-Oligosaccharide Glucosidase (Glucosidase I, MOGS). In: Taniguchi, N., Honke, K., Fukuda, M., Narimatsu, H., Yamaguchi, Y., Angata, T. (eds) Handbook of Glycosyltransferases and Related Genes. Springer, Tokyo. https://doi.org/10.1007/978-4-431-54240-7_10

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