Summary
Enriched populations of adult human Schwann cells were otained from trigeminal ganglia and roots of autopsy material. The cells, isolated by enzymatic procedure, were seeded on rat tail collagencoated coverslips. Subcultures were established several weeks later, and secondary cells were grown on polylysine-coated coverslips and maintained in vitro for as long as 5 months. The Schwann cells in culture displayed the same light- and electron-microscopic features and arrangement as those cells observed in vivo. The addition of bovine pituitary glial growth factor in the medium induced a 3–5-fold increase in Schwann cell division.
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Supported by grants MA 7700 from the Medical Research Council of Canada and 72 (83-1) from the British Columbia Health Care Research Foundation
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Moretto, G., Kim, S.U., Shin, D.H. et al. Long-term cultures of human adult Schwann cells isolated from autopsy materials. Acta Neuropathol 64, 15–21 (1984). https://doi.org/10.1007/BF00695601
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DOI: https://doi.org/10.1007/BF00695601