Summary
The permeability of the endoneurial blood vessels of the sciatic nerve to horseradish peroxidase (HRP) was investigated in mice. The animals were killed 2, 5, or 30 min after an i.v. injection of the protein, and the distribution of HRP in the sciatic nerve was studied by light and electron microscopy. HRP was frequently found in endothelial vesicles that were located near both the luminal and the abluminal side of the endothelium. The intercellular junctions appeared to be closed to HRP. The perivascular and interstitial macrophages in the endoneurium accumulated HRP by endocytosis.
The results are interpreted as evidence for vesicular transport of HRP across endoneurial vessels to interstitial and perivascular macrophages.
Similar content being viewed by others
References
Ahmed AM, Weller RO (1979) The blood-nerve barrier and reconstitution of the perineurium following nerve grafting. Neuropathol Appl Neurobiol 5:469–483
Arvidson B (1977) Cellular uptake of exogenous horseradish peroxidase in mouse peripheral nerve. Acta Neuropathol (Berl) 37:35–41
Arvidson B (1979) Distribution of intravenously injected protein tracers in peripheral ganglia of adult mice. Exp Neurol 63:388–410
Böck P, Hanak H (1971) Die Verteilung exogener Peroxydase im Endoneuralraum. Histochemie 25:361–371
Cecil R, Ogston A (1951) Determination of sedimentation and diffusion constants of horseradish peroxidase. Biochem J 49:105–106
Jacobs JM (1977) Penetration of systemically injected horseradish peroxidase into ganglia and nerves of the autonomic nervous system. J Neurocytol 6:607–618
Jacobs JM, McFarlane RM, Cavanagh JB (1976) Vascular leakage in the dorsal root ganglia of the rat studied with horseradish peroxidase. J Neurol Sci 29:95–107
Jakobsen J, Malmgren L, Olsson Y (1978) Permeability of the blood-nerve barrier in the streptozotocin-diabetic rat. Exp Neurol 60:277–285
Karnovsky MJ (1967) The ultrastructural basis of capillary permeability studied with peroxidase as a tracer. J Cell Biol 35:213–236
Malmgren LT, Olsson Y (1977) A sensitive histochemical method for light-and electron-microscopic demonstration of horseradish peroxidase. J Histochem Cytochem 25:1280–1283
Oldfors A (1980) Macrophages in peripheral nerves. An ultrastructural and enzyme histochemical study in rats. Acta Neuropathol (Berl) 49:43–49
Olsson Y, Reese TS (1969) Inaccessibility of the endoneurium of mouse sciatic nerve to exogenous proteins. Anat Rec 163:318
Olsson Y, Reese TS (1971) Permeability of vasa nervorum and perineurium in mouse sciatic nerve studied by fluorescence and electron microscopy. J Neuropathol Exp Neurol 30:105–119
Rosene DL, Mesulam M-M (1978) Fixation variables in horseradish peroxidase neurohistochemistry. I. The effects of fixation time and perfusion procedures upon enzyme activity. J Histochem Cytochem 26:28–39
Sima AAF, Robertson DM (1978) The perineurial and blood-nerve barriers in experimental diabetes. Acta Neuropathol (Berl) 44:189–195
Waksman BH (1961) Experimental study of diphtheritic polyneuritis in the rabbit and guinea pig. III. The blood-nerve barrier in the rabbit. J Neuropathol Exp Neurol 20:35–77
Author information
Authors and Affiliations
Additional information
Supported by a grant from the Swedish Society of Medical Sciences
Rights and permissions
About this article
Cite this article
Arvidson, B. Evidence for vesicular transport of horseradish peroxidase across endoneurial vessels of the sciatic nerve in normal mice. Acta Neuropathol 64, 1–5 (1984). https://doi.org/10.1007/BF00695598
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00695598