Abstract
2-Hydroxychromene-2-carboxylate isomerase activity was found in cell-free systems from bacteria that degrade naphthalenesulfonates. The enzyme fromPseudomonas testosteroni A3 was activated by incubation with glutathione, dithiothreitol or mercaptoethanol. The highest enzyme activity was found after preincubation of the enzyme with glutathione at alkaline pH-values. A highly purified enzyme preparation converted besides 2-hydroxychromene-2-carboxylate also 2-hydroxybenzo[g]chromene-2-carboxylate (the 2-hydroxychromene-2-carboxylate formed from 1,2-dihydroxyanthracen). The addition of various metal ions or EDTA did not significantly change the catalytic activity of the enzyme. A possible reaction mechanism is proposed.
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Abbreviations
- 2,5-DHCCA:
-
2,5-dihydroxychromene-2-carboxylate
- 2,6-DHCCA:
-
2,6-dihydroxychromene-2-carboxylate
- 1,2-DHN:
-
1,2-dihydroxynaphthalene
- GSH:
-
glutathione
- 2HBCCA:
-
2-hydroxybenzo[g]chromene-2-carboxylate
- HBP:
-
2′-hydroxybenzalpyruvate
- HBPA:
-
2′-hydroxybenzalpyruvate aldolase
- 2HCCA:
-
2-hydroxychromene-2-carboxylate
- 2HCCAI:
-
2-hydroxychromene-2-carboxylate isomerase
- 2NS:
-
naphthalene-2-sulfonate
- Rt :
-
retention time
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Kuhm, A.E., Knackmuss, HJ. & Stolz, A. 2-Hydroxychromene-2-carboxylate isomerase from bacteria that degrade naphthalenesulfonates. Biodegradation 4, 155–162 (1993). https://doi.org/10.1007/BF00695117
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DOI: https://doi.org/10.1007/BF00695117