Summary
Naupliar brine shrimp (Artemia salina) have been used to study the differentiation of salt secretory cells. The effects of several kinds of inhibitors on survival of nauplii at various salinities are presented. Results with ouabain indicate that the ability of the nauplii to survive at high salinities is dependent upon the presence of Na++K+-activated ATPase. Puromycin, chloramphenicol and cycloheximide, all inhibitors of protein synthesis, are more effective at high salt concentrations. Inhibitors of RNA synthesis that are primarily extranuclear in mechanism, i.e. rifamycin and ethidium bromide, also show a marked salt dependency. Among inhibitors which were assayed but did not effect survival under any circumstances were mitomycin C, 5-fluorodeoxyuridine and sarkomycin. Those inhibitors which did not show a differential salt sensitivity were actinomycin D and α-amanitin. It is concluded from these data that coincident transcription and translation of both nuclear and mitochondrial genes are involved in the development of the naupliar salt gland.
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This research was supported by AEC grant RLO 2227-T13-3.
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Ewing, R.D., Peterson, G.L. & Conte, F.P. Larval salt gland ofArtemia salina nauplii. J. Comp. Physiol. 80, 247–254 (1972). https://doi.org/10.1007/BF00694839
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DOI: https://doi.org/10.1007/BF00694839