Skip to main content
SpringerLink
Log in

DNA synthesis during meiosis of eight-spored strains ofChlamydomonas reinhardi

  • Published:
Molecular and General Genetics MGG Aims and scope Submit manuscript

Summary

In strain 137F ofChlamydomonas reinhardi, the zygospores undergo one round of nuclear DNA replication followed by three divisions to produce octospores. The third division without replication has been interpreted by Sueoka et al. (1967, 1969) to mean that the gametes and vegetative cells have at least binemic chromosomes. We have repeated their experiments using the same strain. However, the meiotic products were inviable — unable to undergo postmeiotic vegetative growth, DNA replication or division. On the other hand, using a variant of strain 137C which also has three divisions during germination we have shown that meiosis is normal. Zygospores from this strain undergo two rounds of nuclear DNA replication prior to the formation of octospores. These meiotic products are viable and capable of postmeiotic vegetative growth, replication and division. Since the third division without DNA replication subsequent to the two meiotic divisions leads to inviable products, and the strain which has viable products after three divisions does not lack the additional replication, meiosis inChlamydomonas reinhardi provides no evidence of a bineme chromosome structure.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Chiu, S.M.: Effects of inhibitors of DNA synthesis on recombination. Ph. D. thesis, University of Alberta (1973)

  • Chiu, S. M., Hastings, P.J.: Premeiotic DNA synthesis and recombination inChlamydomonas reinhardi. Genetics73, 29–43 (1973)

    Google Scholar 

  • Davies, D.R., Lawrence, C.W.: Mechanism of recombination inChlamydomonas reinhardi. II. The influence of inhibitors of DNA synthesis on intergenic recombination. Mutation. Res.4, 147–154 (1967)

    Google Scholar 

  • Gibson, D.A., Prescott, D.M.: Sister chromatid exchanges in isolabelling. Exp. Cell Res.83, 445–447 (1973)

    Google Scholar 

  • Hastings, P.J.: The significance of the inviability of meiotic products in an eight-spored strain ofChlamydomonas. Genetics74, slll (1973)

  • Howell, S.H., Walker, L.L.: Informational complexity of the nuclear and chloroplast genomes ofChlamydomonas reinhardi. Biochim. biophys. Acta (Amst.)418, 249–256 (1976)

    Google Scholar 

  • Ikushima, T., Wolff, S.: UV-induced chromatid aberrations in cultured Chinese hamster cells after one, two or three rounds of DNA replication. Mutation Res.22, 193–201 (1974)

    Google Scholar 

  • Kates, J.R., Chiang, K.S., Jones, R.F.: Studies on DNA replication during synchronized vegetative growth and gamete differentiation inChlamydomonas reinhardtii. Exp. Cell Res.49, 121–135 (1968)

    Google Scholar 

  • Kates, J.R., Jones, R.F.: The control of gametic differentiation in liquid cultures ofChlamydomonas. J. cell. comp. Physiol.63, 157–163 (1964)

    Google Scholar 

  • Kirby, K.S.: Isolation of nucleic acids with phenolic solvents.In: Methods in enzymology, Vol. XIIB (L. Grossman and K. Moldave, eds), pp 87–99, New York: Academic Press 1968

    Google Scholar 

  • Mandel, M., Schildkraut, C.L., Marmur, J.: Use of CsCl density gradient analysis for determining the guanine plus cytosine content of DNA.In: Methods in enzymology, Vol. XIIB (L. Grossman and K. Moldave, eds.), pp. 184–195. New York: Academic Press 1968

    Google Scholar 

  • Peacock, W.J.: Chromosome duplication and structure as determined by autoradiography. Proc. nat. Acad. Sci (Wash.)49, 793–801 (1963)

    Google Scholar 

  • Schaechter, M., Delamater, E.D.: Mitosis inChlamydomonas. Amer. J. Bot.42, 417–422 (1955)

    Google Scholar 

  • Sueoka, N., Chiang, K.S., Kates, J.R.: Deoxyribonucleic acid replication in meiosis ofChlamydomonas reinhardi. I. Isotopic transfer experiments with a strain producing eight zoospores. J. molec. Biol.25, 47–66 (1967)

    Google Scholar 

  • Sueoka, N., Chiang, K.S., Kates, J.R.: Meiosis and DNA replication inChlamydomonas. In: Genetics and developmental biology (H.T. Teas, ed.), pp. 25–44, University of Kentucky Press 1969

  • Taylor, J.H.: The organisation and duplication of genetic material. Proc. X. Int. Congr. Genet.1, 63–78 (1959)

    Google Scholar 

  • Taylor, J.H., Woods, P.S., Hughes, W.L.: The organisation and duplication of chromosomes as revealed by autoradiographic studies using tritium-labelled thymidine. Proc. nat. Acad. Sci. (Wash.)43, 122–138 (1957)

    Google Scholar 

  • Trosko, J.E., Wolff, S.: Strandedness ofVicia faba chromosomes as revealed by enzyme digestion studies. J. Cell Biol.26, 125–135 (1965)

    Google Scholar 

  • Wolff, S., Perry, P.: Differential Giemsa staining of sister chromatids and the study of sister chromatid exchanges without autoradiography. Chromosoma (Berl.)48, 341–353 (1974)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Genetics, University of Alberta, T6G 2E9, Edmonton, Alberta, Canada

    C. K. Tan & P. J. Hastings

Authors
  1. C. K. Tan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. P. J. Hastings
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by W. Gajewski

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tan, C.K., Hastings, P.J. DNA synthesis during meiosis of eight-spored strains ofChlamydomonas reinhardi . Molec. Gen. Genet. 152, 311–317 (1977). https://doi.org/10.1007/BF00693085

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00693085

Keywords

Search

Navigation