Abstract
The sexual agglutinin from the mating-type minus gametes ofChlamydomonas reinhardtii was purified by gel filtration and hydroxyapatite chromotography. The minus agglutinin was identified as a single glycopolypeptide termed Agg(-) of very high molecular weight by SDS-poly-acrylamide gel electrophoresis. It was also observed as a glycoprotein with agglutinin activity on non-SDS polyacrylamide gels. The native agglutinin appeared to be composed of a complex of Agg(-) subunits. It consisted of about 60% protein and 40% carbohydrate and the activity was diminished by a mild periodate oxidation. When the plus agglutinin was also purified and compared with the minus agglutinin, it was found that both agglutinins migrate in the same position by SDS-polyacrylamide gel electrophoresis, whereas their behaviors on gel filtration and hydroxyapatite chromatography are different.
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Abbreviations
- mt +/− :
-
mating-tape plus or minus
- SDS:
-
sodium dodecyl sulfate
- Ve :
-
elution volume
- Vo :
-
void volume
- kDa:
-
kilodalton
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Saito, T., Matsuda, Y. Sexual agglutinin of mating-type minus gametes inChlamydomonas reinhardtii . Arch. Microbiol. 139, 95–99 (1984). https://doi.org/10.1007/BF00692720
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DOI: https://doi.org/10.1007/BF00692720