Summary
The occurrence and the distribution of GAGs have been studied histochemically in 224 human cerebral tumors by means of Alcian blue techniques. In the normal peritumoral gray matter the alcianophilia is stronger than in the white matter and demonstrated the presence of HA and CS. In the glioma group the alcianophilia, due to HA and CS, is mainly related to the presence of infiltrated cortex. In the other tumors, GAGs are histochemically disclosed in relation to collagen, reticulin, mesodermic areas, etc. The vessels of every tumor show a positive staining for HA, CS, and HS. The histochemical findings are consistent with the biochemical ones as reported in Part I, even though the significance of GAGs in cerebral tumors remains unknown.
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Arseni C, Carp N, Mestes E, Adel M (1967) Histochemistry of mucopolysaccharides in brain tumors. Acta Neuropathol (Berl) 7:275–284
Böck P, Jellinger K (1981) Detection of glycosaminoglycans in human gliomas by histochemical methods. Acta Neuropathol (Berl) [Suppl] VII:81–84
Costantopoulos G, Iqbal K, Dekaban S (1980) Mucopolysaccharidosis types IH, IS, II, and III A: glycosaminoglycans and lipids of isolated brain cells and other fractions from autopsied tissues. J Neurochem 34:1399–1411
Dawson G (1979) Glycoprotein storage diseases and mucopolysaccharidoses. In: Margolis RU, Margolis RK (eds) Complex carbohydrates of nervous tissue. Plenum Press, New York London, pp 347–375
Derby MA, Pintar JE (1978) The histochemical specificity of streptomyces hyaluronidase and chondroitinase ABC. Histochem J 10:529–547
Dorfman A, Ho PL (1970) Synthesis of acid muco-polysaccharides by glial tumor cells in tissue culture. Proc Natl Acad Sci USA 66:495–499
Engelhardt A (1980) Detection of acid mucopolysaccharides in human brain tumors by histochemical methods. Acta Neuropathol (Berl) 49:199–203
Engelhardt A, Bannasch P (1978) Histochemie saurer Muco-polysaccharide während der Genese Methylnitrosoharnstoff-induzierter Hirntumoren der Ratte. Acta Neuropathol (Berl) 42:197–204
Feigin I (1980) The mucopolysaccharides of the ground substance of the human brain. J Neuropathol Exp Neurol 39:1–12
Giordana MT, Mauro A, Schiffer D (1981) Glycosaminoglycans of brain tumors transplacentally induced by ENU in the rat. Acta Neuropathol (Berl) [Suppl] VII:79–80
Glimelius B, Norling B, Westermark B, Wasteson A (1979) A comparative study of glycosaminoglycans in cultures of human, normal and malignant glial cells. J Cell Physiol 98:527–538
Jourdian GW (1979) Biosynthesis of glycosaminoglycans. In: Margolis RU, Margolis RK (eds) Complex carbohydrates of nervous tissue. Plenum Press, New York London, pp 103–125
Kiang WL, Crockett CP, Margolis RK, Margolis RU (1978) Glycosaminoglycans and glycoproteins associated with microsomal subfractions and liver. Biochemistry 17:3841–3848
Leppl TJ, Stoward PJ (1965) On the use of testicular hyaluronidase for identifying acid mucins in tissue sections. J Histochem Cytochem 13:406–407
Lindahl U, Höök M (1978) Glycosaminoglycans and their binding to biological macromolecules. Ann Rev Biochem 47:385–417
Lovell D, Briggs JC, Schorah CJ (1966) Chemical analysis of acid mucopolysaccharides of mixed salivary tumours. Br J Cancer 20:463–468
Margolis RU, Margolis RK (1974) Distribution and metabolism of mucopolysaccharides and glycoprotein in neuronal perikarya, astrocytes and oligodendroglia. Biochemistry 13:2849–2852
Margolis RU, Margolis RK (1979a) Complex carbohydrates of nervous tissue. Plenum Press, New York London
Margolis RK, Margolis RU (1979b) Structure and distribution of glycoproteins and glycosaminoglycans. In: Margolis RU, Margolis RK (eds) Complex carbohydrates of nervous tissue. Plenum Press, New York London, pp 45–73
Schiffer D, Fabiani A, Vesco C (1961) Considerazioni sugli aspetti morfologico-strutturali dell'oligodendroglioma. Studio istologico su 20 casi. Acta Neurol (Napoli) 16:683–703
Schiffer D, Giordana MT (1974) On the occurrence and significance of acid mucopolysaccharides in oligodendrogliomas experimentally induced in the rat by nitrosourea derivatives. In: Schreiber D, Jänisch W (eds) Experimentelle Neuroonkologie. Barth, Leipzig, pp 101–108
Schiffer D, Giordana MT (1975) Acid mucopolysaccharides in experimental brain tumors. Proceedings of the VIIth International Congress on Neuropathology, Budapest, vol. 1. Excerpta Medica, Amsterdam, pp 533–540
Scott JE, Dorling J (1965) Differential staining of acid glycosamino-glycans (mucopolysaccharides) by Alcian blue in salt solution. Histochemie 5:221–233
Smith B, Butler M (1973) Acid mucopolysaccharides in tumors of the myelin sheath cells, the oligodendroglioma and the neurilemmoma. Acta Neuropathol (Berl) 23:181–185
Spicer SS, Horn RG, Leppi TJ (1967) Histochemistry of connective tissue mucopolysaccharides. In: Smith DE, Wagner BM (eds) The connective tissue. Williams and Wilkins, Baltimore, pp 251–303
Stoolmiller AC, Dawson G, Dorfman A (1973) The metabolism of glycosphingolipids and glycosaminoglycans. In: Sato G (ed) Tissue culture of the nervous system. Plenum Press, New York, pp 247–280
Takeuchi J, Sobue M, Sato E, Shamoto M, Miura K, Nakagaki S (1976) Variation in glycosaminoglycan components of breast tumors. Cancer Res 36:2133–2139
Toledo OMS, Dietrich CP (1977) Tissue specific distribution of sulfated mucopolysaccharides in mammals. Biochim Biophys Acta 498:114–122
Wood JG, McLaughlin BJ (1979) Histochemistry and cytochemistry of glycoproteins and glycosaminoglycans. In: Margolis RU, Margolis RK (eds) Complex carbohydrates of nervous tissue. Plenum Press, New York, pp 139–156
Yamada K (1973) The effect of digestion with streptomices hyaluronidase upon certain histochemical reactions of hyaluronic acid containing tissues. J Histochem Cytochem 21:794–803
Yamada K (1974) The effect of digestion with chondroitinases upon certain histochemical reactions of mucosaccharide-containing tissues. J Neurochem Cytochem 22:266–275
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Supported by CNR Grants N. 81.01 462.96 and N. 81. 01307.96 (Control of neoplastic growth)
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Giordana, M.T., Bertolotto, A., Mauro, A. et al. Glycosaminoglycans in human cerebral tumors. Acta Neuropathol 57, 299–305 (1982). https://doi.org/10.1007/BF00692187
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DOI: https://doi.org/10.1007/BF00692187