Summary
Corpus callosum, cerebellum, and spinal cord from Jimpy mice, and control littermates, 15 and 21 days old, were prepared for freeze-fracture in a “cryofract” apparatus. The few myelinated axons in the Jimpy exhibited a striking paucity of particles in myelin P faces, though tight junctions were present. In addition, small maculae of particles were found on these P faces. Peripheral myelin appeared normal, both for the quantity and disposition of particles on their P faces.
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References
Branton, D.: Fracture faces of frozen membranes. Proc. Natl. Acad. Sci. U.S.A.55, 1048–1056 (1966)
Dermietzel, R.: Junctions in the central nervous system of the cat. III. Gap junctions and membrane-associated orthogonal particle (MOPC) in astrocytic membranes. Cell Tissue Res.149, 121–135 (1974)
Druse, M. J., Hogan, E. L.: Metabolism in vivo of brain galactolipids: The Jimpy mutant. J. Neurochem.19, 2435 (1972)
Escaig, J., Nicolas, G.: Cryo-fractures de matériel biologique réalisées à très basses températures en ultra-vide. C. R. Acad. Sci. [D] Paris283, 1245–1248 (1976)
Fulcrand, J., Privat, A.: Neuroglial reactions secondary to Wallerian degeneration in the optic nerve of the postnatal rat: ultrastructural and quantitative study. J. Comp. Neurol.176, 189–223 (1977)
Hirano, A., Sax, D. S., Zimmerman, H. M.: The fine structure of the cerebella of Jimpy mice and their normal littermates. J. Neuropathol. Exp. Neurol.28, 388–400 (1969)
Landis, D. M., Reese, T. S.: Arrays of particles in freeze-fractured astrocytic membranes. J. Cell Biol.60, 316–320 (1974)
Matthieu, J. M., Quarles, R. H., Webster, H. de F., Hogan, E. L., Brady, R. O.: Characterization of the fraction obtained from the CNS of Jimpy mice by a procedure for myelin isolation. J. Neurochem.23, 517 (1974)
Meier, C., Bischoff, A.: Dysmyelination in “Jimpy” mouse: Electron microscopic study. J. Neuropathol. Exp. Neurol.33, 343 (1974)
Meier, C., Bischoff, A.: Dysmyelination in Jimpy mouse due to astrological hyperplasia? Nature268, 177 (1977)
Miller, R. G., Pinto da Silva, P.: Particle rosettes in the periaxonal Schwann cell membrane and particle clusters in the axolemma of rat sciatic nerve. Brain Res.130, 135–141 (1977)
Schnapp, B., Mugnaini, E.: Freeze-fracture properties of central myelin in the bullfrog. Neuroscience1, 459–467 (1976)
Philipps, R. J. S.: Jimpy, a new totally sex-linked gene in the house mouse. Z. Vererbungslehre86, 322 (1954)
Pinto da Silva, P., Miller, R. G.: Membrane particles on the fracture faces of frozen myelin. Proc. Natl. Acad. Sci. U.S.A.72, 4046–4050 (1975)
Privat, A.: The ependyma and subependymal layer of the young rat: a new contribution with freeze-fracture. Neuroscience2, 447–457 (1977)
Privat, A.: Morphological approaches to the problems of neuroglia. Some comments. In: Dynamics of Neuroglia. Tower, D. B., and Schoffemels, E. (eds.), pp. 55–64. New York: Pergamon 1978
Privat, A., Fulcrand, J.: Neuroglia: from the subventricular precursor to the mature cell. In: Cell, tissue and organculture in neurobiology. Fedoroff, S., and Herz, L. (eds.) New York: Academic Press 1977
Privat, A., Robain, O., Mandel, P.: Aspects ultrastructuraux du corps calleux chez la souris Jimpy. Acta Neuropathol. (Berl.)21, 282–295 (1972)
Rosenbluth, J.: Intramembranous particle distribution at the node of Ranvier and adjacent axolemma in myelinated axons of the frog brain. J. Neurocytol.5, 731–745 (1976)
Sidman, R. L., Hayes, R.: Jimpy: A mouse with inherited sudanophilic leukodystrophy. J. Neuropathol. Exp. Neurol.24, 172 (1965)
Skoff, R. P.: Myelin deficit in the Jimpy mouse may be due to cellular abnormalities in astroglia. Nature264, 560–562 (1976)
Tani, E., Kimiyuki, I., Nishiura, M.: Freeze-etching images of central myelinated nerve fibres. J. Neurocytol.2, 305–314 (1973)
Torii, J., Adachi, M., Volk, B. W.: Histochemical and ultrastructural studies of experimental leucodystrophy in mice. J. Neuropathol. Exp. Neurol.30, 278–289 (1971)
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Privat, A., Drian, M.J. & Escaig, J. Jimpy mouse myelin revisited with freeze-fracture. Acta Neuropathol 45, 129–131 (1979). https://doi.org/10.1007/BF00691890
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DOI: https://doi.org/10.1007/BF00691890