Summary
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1.
The oxidative phosphorylation and the temperature dependence of mitochondria prepared from the outer compact layer and the inner spongy layer of adult tuna heart ventricle have been examined.
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2.
Mitochondria of the inner layer show higher succinoxidase and NADH-oxidase activities as compared with those of the outer layer.
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3.
Arrhenius plots for succinate oxidation by phosphorylating mitochondria show that the temperature dependence of the inner layer is higher than that of the outer layer.
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4.
Experiments performed with disrupted non-phosphorylating mitochondria demonstrate that this difference in temperature dependence of the two cardiac compartments depends on the integrity of the mitochondrial membranes.
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5.
These findings are discussed in relation to the physiology of the fish.
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Maresca, B., Modigh, M., Servillo, L. et al. Different temperature dependences of oxidative phosphorylation in the inner and outer layers of tuna heart ventricle. J Comp Physiol B 105, 167–172 (1976). https://doi.org/10.1007/BF00691118
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DOI: https://doi.org/10.1007/BF00691118