Abstract
A bacitracin-producing strainBacillus licheniformis ATCC 10716 harbors two types of inducible phages (LP52 and DLP 10716). 156 strains re-lysogenized with phage LP52 were independently isolated from a cured strain UM12 ofB. licheniformis. Those strains were divided into 12 groups based on colony morphology and pigment production. Some of the re-lysogenized strains grew faster than UM12 and others produced more bacitracin than the cured strain. For example, the production of bacitracin by one of the relysogenized strains, L89, was enhanced by about 70% in comparison with UM12. The phenotypic variations observed with re-lysogenized strains might be due to the re-insertion of the phage genome at different sites of the chromosome in addition to the pleiotropic effect assumed.
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Abbreviations
- ATCC:
-
American Type Culture Collection
- DNA:
-
Deoxyribonucleic acid
- MC:
-
Mitomycin C
- OD:
-
Optical density
- PFU:
-
Plaque forming unit
- rpm:
-
Revolutions per minute
- UOD:
-
Unit of optical density
- UV:
-
Ultraviolet
- \(\mu = \frac{1}{X}\frac{{dX}}{{dt}}\) :
-
Specific growth rate (h-1)
- t :
-
time (h)
- X :
-
cell concentration (g/l)
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Imanaka, T., Uchida, K. & Aiba, S. Phenotypic variations in re-lysogenization ofBacillus licheniformis with temperate phage. Arch. Microbiol. 132, 75–78 (1982). https://doi.org/10.1007/BF00690821
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DOI: https://doi.org/10.1007/BF00690821