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Acta Neuropathologica

, Volume 47, Issue 3, pp 175–181 | Cite as

Alterations in cat cerebrocortical capillary morphometrical parameters following K+-induced cerebrocortical swelling

  • E. L. Auen
  • R. S. Bourke
  • K. D. Barron
  • B. D. San Filippo
  • J. B. Waldman
Original Works

Summary

Histochemical, electron microscopic, and morphometrical techniques were employed in the determination of the effects attributed to K+-induced cerebrocortical swelling on cat cerebrocortical capillary diameter, length, surface area, volume, and minimal intercapillary distance.

Bilaterally exposed and intact temporoparietal cerebral cortices of 4 conditioned adult cats were simultaneously superfused with isotonic, artificial CSF containing 3.5 mM K+ (control) and 54 mM K+ (experimental) for 1 h at 37°C with monitoring of systemic vital function, hematocrit, arterial blood gases, and determination of cerebrocortical tissue water content.

The mean values for cerebrocapillary diameter were 5% (P<0.05) greater in swollen tissues when compared with comparable mean values determined for controls. The values for minimal intercapillary distance determined from control and experimental animals plotted as relative frequency histograms represented two distinct populations (P<0.0005). The significance of altered capillary morphometric parameters are discussed in relation to K+-induced cerebrocortical swelling.

Key words

Brain capillaries Brain cortex Cat brain Astroglial swelling Brain edema Brain extracellular potassium Morphometry 

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References

  1. Barron, K. D.: Enzyme histochemistry of central nervous system. In: Minckler, J. (ed.). Pathology of the nervous system. Vol. 1, pp. 177–227. Philadelphia: McGraw-Hill 1968Google Scholar
  2. Bicher, H. I., Reneau, D. A., Knisely, M. H.: In: Hershey, D. (ed.), Blood oxygenation. pp. 219–231. New York: Plenum Press 1970Google Scholar
  3. Boretos, J. W., Bourke, R. S., Nelson, K. M., Naumann, R. A., Ommaya, A. K.: Technique for unilateral isolation of the subdural space in intact primate. J. Neurosurg.35, 101–107 (1971)Google Scholar
  4. Bourke, R. S., Nelson, K. M.: Further studies on the K+ dependent swelling of primate cerebral cortex in vivo: The enzymatic basis of the K+ dependent transport of chloride. J. Neurochem.19, 663–685 (1972a)Google Scholar
  5. Bourke, R. S., Nelson, K. M.: Studies on the site of mediated transport of chloride from blood into cerebrospinal fluid: Effects of acetazolamide. J. Neurochem.19, 1225–1232 (1972b)Google Scholar
  6. Bourke, R. S., Tower, D. B.: Fluid compartmentation and electrolytes of cat cerebral cortex in vitro. II. J. Neurochem.13, 1099–1117 (1966)Google Scholar
  7. Bourke, R. S., Nelson, K. M., Naumann, R. A., Young, O. M.: Studies of the production and subsequent reduction of swelling in primate cerebral cortex under isosmotic conditions in vivo. Exp. Brain Res.10, 427–446 (1970)Google Scholar
  8. Bourke, R. S., Kimelberg, H. K., West, C. R., Bremer, A. M.: The effect of HCO3 on the swelling and ion uptake of monkey cerebral cortex under conditions of raised extracellular potassium. J. Neurochem.25, 322–328 (1975)Google Scholar
  9. Bourke, R. S., Daze, M. A., Kimelberg, H. K.: Chloride transport in mammalian astroglia. In: Schoffeniels, F., Franck, G., Hertz, L., Tower, D. B. (eds.) Dynamic properties of glia cells. Oxford, New York:Pergamon Press 1978aGoogle Scholar
  10. Bourke, R. S., Kimelberg, H. K., Dazé, M. A.: Effects of inhibitors and adenosine on (HCO3/CO2)-stimulated swelling and Cl uptake in brain slices and cultured astrocytes. Brain Res.154, 192–202(1978b)Google Scholar
  11. Culling, C. F. A.: Handbook of histopathological and histochemical techniques, 3rd ed. pp. 319–323. Toronto, Canada: Butterworth 1974Google Scholar
  12. Elliot, K. A. C.: Brain tissue respiration and glycosis. In:Elliot, K. A. C., Page, I. H., Quastel, J. I. (eds.) Neurochemistry, pp. 63–93. Springfield, IL: Thomas 1955Google Scholar
  13. Elliot, K. A. C., Jasper, H.: Measurement of experimentally induced brain swelling and shrinkage. Am. J. Physiol.157, 122–129 (1949)Google Scholar
  14. Fisher, R. A., Pedley, T. A., Prince, D. A.: Kinetics of potassium movement in normal cortex. Brain Res.101, 223–237 (1976)Google Scholar
  15. Garcia, J. H., Kalimo, H., Kamijyo, Y., Trump, B. F.: Cellular events during early cerebral ishemia. I. Electron microscopy of feline cerebral cortex after middle-cerebral-artery occlusion. Virchows Archiv [Cell Pathol.]25, 191–206 (1977)Google Scholar
  16. Hossmann, K.-A., Sakaki, S., Zimmerman, V.: Cation activities in reversible ischemia of the cat brain. Stroke8, 77–81 (1977)Google Scholar
  17. Hunziker, O., Emmenegger, H., Frey, H., Schulz, U., Meier-Ruge, W.: Morphometric characterization of the capillary network in the cat's brain cortex: A comparison of the physiological state and hypovolemic conditions. Acta Neuropathol. (Berl.)29, 57–63 (1974a)Google Scholar
  18. Hunziker, O., Frey, H., Schulz, U.: Morphometric investigations of capillaries in the brain cortex of the cat. Brain Res.65, 1–11 (1974b)Google Scholar
  19. Hunziker, O., Schweizer, A.: Postmortem changes in stereological parameters of cerebral capillaries. Beitr. Pathol.161, 244–255 (1977)Google Scholar
  20. Jenkner, F. L.: Über die anticholinerge Therapie der schweren geschlossenen Schädelhirntraumen. Langenbecks Arch. Klin. Chir.286, 91–98 (1957)Google Scholar
  21. Karlsson, Y., Schultz, R. L.: Fixation of the central nervous system for electron microscopy by aldehyde perfusion. J. Ultrastruct. Res.12, 160–186 (1965)Google Scholar
  22. Kirshner, H. S., Blank, W. F., Jr., Myers, R. E.: Changes in cortical subarachnoid fluid potassium concentrations during hypoxia. Arch. Neurol.33, 84–90 (1976)Google Scholar
  23. Lund-Anersen, H., Hertz, L.: Effects of potassium and of glutamate on swelling and on sodium and potassium content in brain cortex slices from adult rats. Exp. Brain Res.11, 199–212 (1970)Google Scholar
  24. Maynard, E. A., Schultz, R. L., Pease, D. C.: Electron microscopy of the vascular bed of the rat cerebral cortex. Am. J. Anat.100, 409–433 (1957)Google Scholar
  25. Ommaya, A. J., Boretos, J. W., Beile, E. E.: The lexan calvarium: An improved method for direct observation of the brain. J. Neurosurg.30, 25–29 (1969)Google Scholar
  26. Sabatini, D. D., Bensch, K., Barrnett, J. R.: Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation. J. Cell Biol.17, 19–58 (1963)Google Scholar
  27. Silver, I. A.: Some observations on the cerebral cortex with an ultramicro, membrane-covered, oxygen electrode. Med. Electron. Biol. Eng.3, 377–387 (1965)Google Scholar
  28. Snedecor, G. W., Chochran, W. G.: Statistical methods, 6th ed. Ames, IA: The Iowa State University Press 1967Google Scholar
  29. Snider, R. S., Niemer, W. T.: A stereotaxic atlas of the cat brain, 2nd ed. Chicago, London: The University of Chicago Press 1964Google Scholar
  30. Thews, G. In: Schade, J. P., McMenemmy, W. H. (eds.) Selective vulnerability of the brain, pp. 27–40 Oxford: Blackwell 1963Google Scholar
  31. Tower, D. B.: The effects of 2-deoxy-D-glucose on metabolism of slices of cerebral cortex incubated in vitro. J. Neurochem.3, 185–205 (1958)Google Scholar
  32. Vyskocil, F., Kriz, N., Bures, J.: Potassium-selective microelectrodes used for measuring the extracellular brain potassium during spreading depression and anoxic depolarization in rats. Brain Res.39, 255–259 (1972)Google Scholar
  33. Weibel, E. R., Fisher, C., Gahm, J., Schaefer, A.: Current capabilities and limitations of available stereological techniques. J. Microsc.95, 367–392 (1972)Google Scholar
  34. West, C. R., Matsen, F. A.: Effects of experimental ischemia on electrolytes of cortical cerebrospinal fluid and on brain water. J. Neurochem.36, 687–699 (1972)Google Scholar
  35. Wiederhold, K. H., Bielser, W., Schultz, U., Veteau, M., Hunziker, O.: Three dimensional reconstruction of brain capillaries from frozen serial sections. Microvasc. Res.11, 175–180 (1976)Google Scholar

Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • E. L. Auen
    • 1
  • R. S. Bourke
    • 3
  • K. D. Barron
    • 2
  • B. D. San Filippo
    • 3
  • J. B. Waldman
    • 3
  1. 1.Department of AnatomyAlbany Medical CollegeAlbanyUSA
  2. 2.Department of Neurology and PathologyAlbany Medical CollegeAlbanyUSA
  3. 3.Division of NeurosurgeryAlbany Medical CollegeAlbanyUSA

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