Summary
The cytomorphology and cytogenesis of BK virus-transformed hamster brain cells (HBBK cells) were studied. HBBK cells with indistinct processes showed a tendency to epithelial arrangement as well as characteristics of malignant tumor cells in vitro, and developed long, delicate processes when cell growth was suppressed. Glial fibrillary acidic protein was demonstrated by immunofluorescence in the cytoplasm of almost all HBBK cells. The tumors arising in hamsters after subcutaneous inoculation of HBBK cells were classified as poorly differentiated gliomas.
These findings suggest that selective transformation of glial cells in hamster brain cells by BK virus occurred.
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Bissell, M. G., Rubinstein, L. J., Bignami, A., Herman, M. M.: Characteristics of the rat C-6 glioma maintained in organ culture systems. Production of glial fibrillary acidic protein in the absence of gliofibrillogenesis. Brain Res.82, 77–89 (1974)
Brightman, M. W., Reese, T. S.: Junctions between intimately opposed cell membranes in the vertebrate brain. J. Cell Biol.40, 648–677 (1969)
Burger, P. C., Bigner, D. D., Self, D. J.: Morphologic observations of brain tumors in PD4 hamsters induced by four strains of avian sarcoma virus. Acta neuropath. (Berl.)26, 1–21 (1973)
Costa, J., Yee, C., Tralka, T. S., Rabson, A. S.: Hamster ependymomas produced by intracerebral inoculation of a human papovavirus (MMV). J. nat. Cancer. Inst.56, 863–864 (1976)
Eng, L. F., Vanderhaeghen, J. J., Bignami, A., Gerstl, B.: An acidic protein isolated from fibrous astrocytes. Brain Res.28, 351–354 (1971)
Gardner, S. D., Field, A. M., Coleman, D. V., Hulme, B.: New human papovavirus (BK) isolated from urine after renal transplantation. Lancet1, 1253–1257 (1971)
Gardner, S. D.: Prevalence in England of antibody to human polyomavirus (BK). Brit. med. J.1, 77–78 (1973)
Millonig, G.: Advantages of a phosphate buffer for osmium tetroxide solution in fixation. J. appl. Physiol.32, 1637 (1961)
Ogawa, K., Hayama, K., Fujii, Y., Matsuura, K., Endo, T.: Tumor induction by adenovirus type 12 and its target cells in the central nervous system. Gann60, 383–392 (1969)
Padgett, B. L., Walker, D. L., ZuRhein, G. M., Eckroade, R. J., Dessel, B. H.: Cultivation of papova-like virus from human brain with progressive multifocal leucoencephalopathy. Lancet1, 1257–1260 (1971)
Padgett, B. L., Walker, D. L.: Prevalence of antibodies in human sera against JC virus, an isolate from a case of progressive multifocal leucoencephalopathy. J. infect. Dis.127, 467–470 (1973)
Rorke, L. B., Gilden, D. H., Wroblewska, Z., Santoli, D.: Human brain in tissue culture. IV. Morphological characteristics. J. comp. Neurol.161, 329–340 (1975)
Sato, S., Sugimura, T., Yoda, K., Fujimura, S.: Morphological differentiation of cultured mouse glioblastoma cells induced by dibutyryl cyclic adenosine monophosphate. Cancer Res.35, 2494–2499 (1975)
Shah, K. V., Daniel, R. W., Strandberg, J. D.: Sarcoma in a hamster inoculated with BK virus, a human papovavirus. J. nat. Cancer Inst.54, 945–950 (1975)
Shein, H. M.: Neoplastic transformation induced by simian virus 40 in Syrian hamster neuroglial and meningeal cell cultures. Arch. ges. Virusforsch.22, 122–142 (1967)
Silbert, S. W., Goldstein, M. N.: Drug-induced differentiation of a rat glioma in vitro. Cancer Res.32, 1422–1427 (1972)
Tanaka, R., Koprowski, H., Iwasaki, Y.: Malignant transformation of hamster brain cells in vitro by human papovavirus BK. J. nat. Cancer Inst.56, 671–673 (1976)
Walker, D. L., Padgett, B. L., ZuRhein, G. M., Albert, A. E., Marsh, R. F.: Human papovavirus (JC): Induction of brain tumors in hamsters. Science181, 674–676 (1973)
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Supported in part by Public Health Service grants CA 10815 from the National Cancer Institute
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Tanaka, R., Sekiguchi, K., Ueki, K. et al. Morphological studies of hamster brain cells transformed in vitro by a human papovavirus BK. Acta Neuropathol 41, 235–239 (1978). https://doi.org/10.1007/BF00690442
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DOI: https://doi.org/10.1007/BF00690442