Summary
A single injection of ovine TSH (1.1 μg or 2.2 μg/g body weight) effected a significant (P<0.01) increase in plasma T4 levels in rainbow trout within 9 h and 1 h respectively. In fish given the higher dose the T4 levels were still significantly higher (P<0.01) than in the controls 24 h after the injection. Ovine TSH had no significant effect on plasma T3 levels. Plasma T3/T4 ratios in fish given the higher TSH dose were significantly lower than the controls at 3 h (P<0.01), 6 h (P=0.01), 9 h and 24 h (P<0.05) while in fish given the lower dose they were significantly lower than in controls at 3 h (P<0.01), 6 h (P=0.01) and 9 h (P<0.05).
T4 levels in TSH-injected fish were significantly higher (P<0.05) than in controls after 3 and 11 injections but were not significantly different after 16 injections. Plasma T3 and T3/T4 ratios did not differ significantly in fish given multiple injections of either TSH or saline.
Plasma T4 levels in fish treated with thiourea were significantly lower (Pº0.01) than in comparable controls after 6, 9 and 15 days and plasma T3 levels were lower (P<0.05) after 15 days treatment. T3/T4 ratios did not differ significantly at any time period.
A single injection of bovine STH (10 μg/g body weight) effect a significant rise in plasma T4 levels 9 h (P=0.05) and 24 h (P<0.01) after the injection while ovine prolactin in a similar dose was without effect. Plasma T3 levels in saline injected fish showed a steady decline over the 24 h following the injection whereas levels in prolactin and STH injected animals tended to rise. T3 levels in the saline-injected fish were significantly higher (P<0.01) than in prolactin and STH injected animals 1 h after the injection but were significantly lower (P<0.01) than in the hormone-treated fish 24 h after the injection. There were no significant differences in T3/T4 ratios in any of the groups. Multiple injections of STH effected a significant increase (P<0.05) in plasma T4 compared with the controls; prolactin had no significant effect. There was no significant differences in plasma T3 concentration or T3/T4 ratios in any of the groups.
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Milne, R.S., Leatherland, J.F. Effect of ovine TSH, thiourea, ovine prolactin and bovine growth hormone on plasma thyroxine and tri-iodothyronine levels in rainbow trout,Salmo gairdneri . J Comp Physiol B 124, 105–110 (1978). https://doi.org/10.1007/BF00689169
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DOI: https://doi.org/10.1007/BF00689169