Summary
A study of the thermal responses of Na-ATPase and NaK-ATPase activities in microsomes prepared from gill tissue of rainbow trout (Salmo gairdneri) revealed further evidence that the two activities are distinct from one another. Arrhenius plots of the NaK-ATPase from sea water-adapted fish and the Na-ATPase from fresh water-adapted fish were linear (Fig. 4) with estimated activation energies of 19.5 and 7.7 kcal/mole, respectively. The Na-ATPase and NaK-ATPase both showed optimum activity at 45°C (Figs. 2 and 3). The Mg-ATPase from fresh water fish showed a distinct temperature optimum at 24°C (Fig. 1) while Mg-ATPase activity from sea water fish was optimum at temperatures of about 15–24 °C (Fig. 3). The Na+ dependence of the Na-ATPase and the NaK-ATPase was examined at an assay temperature of 37 °C (Fig. 5) and the results compared with those obtained at 13 °C. No apparent differences were noted for the Na-ATPase, but with the NaK-ATPase both theK 0.5 for Na+ and optimum Na+ concentration increased at the higher assay temperature. Finally, evidence is presented showing the Na-ATPase to be distinct from Mg-ATPase activity in fresh water trout gill microsomes.
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Abbreviations
- HEPES:
-
N-2-hydroxyethylpiperazine-N′-2-ethane-sulfonic acid
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Pfeiler, E. Na-ATPase and NaK-ATPase activities in gills of rainbow trout (Salmo gairdneri). J Comp Physiol B 124, 97–104 (1978). https://doi.org/10.1007/BF00689168
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DOI: https://doi.org/10.1007/BF00689168