Summary
Crude extracts ofGillichthys urophyses and chromatographically purified urotensin I (UI) and urotensin II (UII) (fromCatostomus urophyses) were injected intravenously intoCoturnix coturnix japonica, Colinus virginianus, Alectoris graeca (Galliformes) andColumba livia (Columbiformes). Changes in arterial blood pressure were monitored. UI elicited dose-dependent vasodepressor responses in all birds. Thioglycollate treatment abolished the depressor action of arginine vasotocin but not that of UI (in all birds). UII was a pressor agent inCoturnix andColinus, both members of the Galliformes. However, the hormone had no pressor activity inColumba, a member of the Columbiformes, and in another member of the Galliformes,Alectoris. The pressor effect of UII was also dose-dependent. Injection of crude urophysial extract intoColinus andCoturnix, therefore, elicited biphasic responses. UII effects can be abolished by prior incubation with carboxypeptidase A. Intravenous injection of the α-adrenoceptor blocker, phenoxybenzamine, prior to urotensin injections had no effect on the responses to urotensins. As the chukar,Alectoris graeca, is sensitive to UI and resistant to anesthesia and surgery, and does not readily develop tachyphylaxis to repeated UI injections, its use is recommended as a routine bioassay animal for UI.
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References
Amin, A.H., Crowford, T.B.B., Gaddum, J.H.: The distribution of substance P and 5-hydroxytryptamine in the central nervous system of the dog. J. Physiol.126, 596–618 (1954)
Bern, H.A., Lederis, K.: A reference preparation for the study of active substances in the caudal neurosecretory system of teleosts. J. Endocrinol.45 xi-xii (1969)
Bern, H.A., Lederis, K.: The caudal neurosecretory system of fishes. In: Neurosecretion and neuroendocrinology (eds. W. Bargmann, A. Oksche, A. Polenov, B. Scharrer). Berlin-Heidelberg-New York: Springer (in press) 1978
Bern, H.A., Nishioka, R.S., Chester Jones, I., Chan, D.K.O., Rankin, J.C., Ponniah, S.: The urophysis of teleost fish. J. Endocrinol.37, xl-xli (1967)
Bissett, G.W.: The assay of oxytocin and vasopressin in blood and the mechanism of inactivation of these hormones by sodium thioglycollate. In: Oxytocin (eds. R. Caldeyro-Barcia, H. Heller), pp. 380–399. Oxford: Pergamon 1961
Bree, M.M., Gross, N.B.: Anesthesia of pigeons with Cl 581 (ketamine) and pentobarbital. Lab. Anim. Care19, 500–502 (1969)
Chan, D.K.O.: Cardiovascular and renal effects of urotensins I and II in the eel,Anguilla rostrata. Gen. Comp. Endocrinol.27, 52–61 (1975)
Chan, D.K.O., Bern, H.A.: The caudal neurosecretory system: a critical evaluation of the two-hormone hypothesis. Cell Tiss. Res.174, 339–354 (1976)
Chan, D.K.O., Gunther, R., Bern, H.A.: The isolated trout rectum bioassay for urotensin II: assessment for specificity and precision. Gen. Comp. Endocrinol.34, 347–359 (1978)
Kobayashi, H., Matsui, T., Hirano, T., Iwata, T., Ishii, S.: Vasodepressor substance in fish urophysis. Annot. Zool. Japon.41, 154–158 (1968)
Lacanilao, F.: The urophysial hydrosmotic factor of fishes. I. Characteristics and similarity to neurohypophysial hormones. Gen. Comp. Endocrinol.19, 405–420 (1972a)
Lacanilao, F.: The urophysial hydrosmotic factor of fishes. II. Chromatographic and pharmacologic indication of similarity to arginine vasotocin. Gen. Comp. Endocrinol.19, 413–420 (1972b)
Lacanilao, F., Bern, H.A.: The urophysial hydrosmotic factor of fishes. III. Survey of fish caudal spinal cord regions for hydrosmotic activity. Proc. Soc. Exp. Biol. Med.140, 1252–1253 (1972)
Lederis, K.: Active substance in the caudal neurosecretory system of bony fishes. Mem. soc. Endocrinol.18, 465–484 (1971)
Lederis, K.: Chemical properties and the physiological and pharmacological actions of urophysial peptides. Amer. Zool.17, 823–832 (1977)
Lederis, K., Bern, H.A., Nishioka, R.S., Geschwind, I.I.: Some observations on biological and chemical properties and subcellular localization of urophysial active principles. Mem. Soc. Endocrinol.19, 413–433 (1971)
Lederis, K., Letter, A., Moore, G.: Chemical and biological properties of urotensins I and II. Gen. Comp. Endocrinol.29, 245 (1976)
Lederis, K., Medakovic, M.: Pharmacological observations on the hypotensive action of extracts of teleost fish urophysis (urotensin I) in the rat. Brit. J. Pharmacol.51, 315–324 (1974a)
Lederis, K., Medakovic, M.: Effects and assay of UI on the perfused hind limb of the rat. Gen. Comp. Endocrinol.24, 10–16 (1974b)
MacCannell, K., Lederis, K.: Dilatation of the mesenteric vascular bed of the dog produced by a peptide, urotensin I. J. Pharm. Exp. Ther203, 38–46 (1977)
Medakovic, M., Chan, D.K.O., Lederis, K.: Pharmacological effects of urotensins. I. Regional vascular effects of urotensins I and II in the rat. Pharmacol.13, 409–418 (1975a)
Medakovic, M., Chan, D.K.O., Lederis, K.: Pharmacological effects of urotensins. II. Renal effects of urotensin I in the rat. Pharmacol.13, 419–426 (1975b)
Medakovic, M., Chan, D.K.O., Lederis, K., Gosbeen, J.L.: Antidiuretic effects of urotensin 1. Proc. Can. Fed. Biol. Soc.16, 116 (1973)
Medakovic, M., Devlin, A., Lederis, K.: Blood pressure and renal effects of prolonged daily subcutaneous administration of urotensin I peptide in spontaneously hypertensive rats. Proc. West. Pharmacol. Soc.18, 384–388 (1975)
Medakovic, M., Lederis, K.: Pharmacological effects of urotensins IV. Blood pressure lowering effects of urotensin I in spontaneously hypertensive rats. Pharmacol.13, 433–440 (1975)
Moore, G., Letter, A., Lederis, K.: Pharmacological and chemical properties of urotensin I and II peptides from the caudal neurosecretory system of bony fish. In: Peptides: Chemistry, structure and biology (eds. r. Walter, J. Meienhofer), pp. 589–595. Ann Arbor, Michigan: Ann Arbor Science 1975
Zelnik, P.R., Lederis, K.: Chromatographic separation of urotensins. Gen. Comp. Endocrinol.20, 392–400 (1973)
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Woo, N.Y.S., Bern, H.A. Vasoactive properties of urotensins I and II in a columbiform and three galliform birds, and a bioassay for urotensin I. J Comp Physiol B 126, 193–202 (1978). https://doi.org/10.1007/BF00688928
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DOI: https://doi.org/10.1007/BF00688928