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Journal of comparative physiology

, Volume 148, Issue 1, pp 101–109 | Cite as

Characterization of an invertebrate transferrin from the crabCancer magister (Arthropoda)

  • Helmut A. Huebers
  • Eiko Huebers
  • Clement A. Finch
  • Arthur W. Martin
Article

Summary

An iron binding protein was isolated from crab blood and tested for its chemical and functional behavior.
  1. 1.

    The protein has a molecular weight of 150,000±10,000 and consists of a single polypeptide chain. The iron content was found to be two molecules iron per molecule protein; the presence of a 52-fold molar excess of copper did not alter the iron binding properties.

     
  2. 2.

    Spectroscopical analysis carried out in the presence of bicarbonate yielded two absorption maxima at λ=276 and λ=465 nm.

     
  3. 3.

    The59Fe-tagged crab protein did not yield its iron to human apotransferrin when incubated in human plasma. When incubated with rat reticulocytes, the crab iron protein delivered its iron for hemoglobin and ferritin synthesis. This delivery can be completely abolished by adding an excess of rat transferrin.

     
  4. 4.

    When injected into rats in vivo, the crab protein delivered its iron to erythroid and non-erythroid tissue. The amount delivered to the erythroid marrow was shown to depend on iron requirements of that tissue.

     
  5. 5.

    When injected as59Fe-tagged protein into a crab, half-time disappearance of the radioiron is reached between 7 and 9 h. In the liver and in the carapace lining, the radioiron released is found in other iron binders.

     
  6. 6.

    Except for the significantly larger molecular weight, the crab iron binding protein fulfills all the criteria for transferrin. The findings negate the concept that transferrin is a newcomer in the evolutionary scene and to be found only in the phylum Chordata. Thus the need for a specific iron transport protein of the transferrin class is independent of the achievement of hemoglobin as a means to accomplish oxygen transport.

     

Keywords

Ferritin Transferrin Single Polypeptide Chain Iron Binding Protein Iron Requirement 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviation

UIBC

unsaturated iron binding capacity

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Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • Helmut A. Huebers
    • 1
  • Eiko Huebers
    • 1
  • Clement A. Finch
    • 1
  • Arthur W. Martin
    • 1
  1. 1.Departments of Medicine and ZoologyUniversity of WashingtonSeattleUSA

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