Summary
-
1.
By use of histochemical methods the activity and localization of oxo-reductive enzymes and certain hydrolytic enzymes in chick embryo choroid plexus in between the 35 to 46th stage of development was studied.
-
2.
An increase of enzyme activity with increasing age was found in the choroid plexus with the exception of cytochrome oxidase, which revealed activity of a fairly constant level.
-
3.
The localization of oxoreductive and hydrolytic enzymes on the brush borders of epithelial cells of the choroid plexus especially at the 40th stage of development was prominent.
-
4.
The localization of the various enzyme systems may be related to the active transport phenomena in the choroid plexus.
Zusammenfassung
-
1.
Mittels histochemischer Methoden wurden Aktivität und Lokalisation von oxoreduktiven und gewissen hydrolytischen Enzymen im Plexus chorioideus des Hühnerembryo zwischen dem 35. und 46. Entwicklungsstadium untersucht.
-
2.
Ein Anstieg der Enzymaktivität mit zunehmendem Alter wurde im Plexus chorioideus gefunden; eine Ausnahme bildete die Cytochrom-oxydase, die eine ungefähr gleichbleibende Aktivität zeigte.
-
3.
Auffallend war die Lokalisation der oxoreduktiven und hydrolytischen Enzyme im Bürstenbesatz der Epithelzellen des Plexus chorioideus,-vornehmlich im 40. Entwicklungsstadium.
-
4.
Die Lokalisation verschiedener Enzymsysteme könnte mit den Phänomenen des aktiven Transportes im Plexus chorioideus in Verbindung stehen.
Similar content being viewed by others
References
Bering, E. A., jr.: Circulation of the cerebrospinal fluid. Demonstration of the choroid plexus as generator of the force for flow of fluid and ventricular enlargement. J. Neurosurg.19, 405–413 (1962).
Bourne, G. H.: Histochemical demonstration of phosphatases in the central nervous system of the rat. Exp. Cell Res. Suppl.5, 101–117 (1958).
Brand, L., andH. R. Mahler: Biochemical studies of developing avian embryos. III. The oxidation of reduced pyridine nucleotide. J. biol. Chem.234, 1615–1624 (1959).
Burstone, M. S.: Comparison of naphthol AS-phosphates for demonstration of phosphatases. J. nat. Cancer Inst.20, 601–615 (1958).
Burstone, M. S.: Histochemical demonstration of acid phosphatase. J. nat. Cancer Inst.21, 523–539 (1958).
—: Histochemical demonstration of cytochrome oxidase with new amine reagents. J. Histochem. Cytochem.8, 63–70 (1960).
—: Histochemical demonstration of phosphatases in frozen sections with naphthol AS-phosphates. J. Histochem. Cytochem.9, 146–153 (1961).
—: Enzyme Histochemistry and Its Application in the Study of Neoplasms. New York: Academic Press 1962.
Conklin, J. L., M. M. Dewey, andR. H. Kahn: Cytochemical localization of certain oxidative enzymes. Am. J. Anat.110, 19–27 (1962).
Conway, E. J.: Significance of various factors including lactic dehydrogenase on the active transport of sodium ions in skeletal muscle. Nature (Lond.)198, 760–763 (1963).
Dandy, W. E.: Experimental hydrocephalus. Ann. Surg.70, 129–142 (1919).
Davidson, J.: Activity of certain metabolic enzymes during development of the chick embryo. Growth21, 287–295 (1957).
Fisher, R. G., andJ. H. Copenhaver: The metabolic activity of the choroid plexus. J. Neurosurg.16, 167–176 (1959).
Flexner, L. B.: The chemistry and nature of the cerebrospinal fluid. Physiol. Rev.14, 161–187 (1934).
—, andR. D. Stiehler: Biochemical changes associated with onset of secretion in the fetal choroid plexus. An organization of oxidation-reduction processes. J. biol. Chem.126, 619–626 (1938).
Friedenwald, J. S., H. Hermann, andR. Buka: The distribution of certain oxidative enzymes in the choroid plexus. Johns Hopk. Hosp. Bull.20, 1–13 (1942).
Hamburger, V., andH. L. Hamilton: A series of normal stages in the development of the chick embryo. J. Morph.88, 49–86 (1951).
Hokin, L. E., andM. R. Hokin: Studies on the enzymatic mechanism of the sodium pump. Membrane Transport and Metabolism, ed. byA. Kleinzeller andA. Kotyk, p. 204–217. New York: Academic Press 1961.
Krebs, H. A., andH. Rosenhagen: Über den Stoffwechsel des Plexus Choroideus. Zbl. ges. Neurol. Psychiat.134, 643–657 (1931).
Leduc, E. H., andG. B. Wislocki: The histochemical localization of acid and alkaline phosphatases, nonspecific esterase and succinic dehydrogenase in the structures comprising the hematocephalic barrier of the rat. J. comp. Neurol.97, 241–266 (1952).
Mahler, H. R.: Biochemical studies of the developing avian embryo. II. Enzymes of the citric acid cycle. J. biol. Chem.233, 770–788 (1958).
Moog, F.: Differentiation of enzymes in relation to the functional activities of developing embryo. Ann. N. Y. Acad. Sci.55, 57–66 (1952).
Nachlas, M. M., K. C. Tsou, E. Souza, C. H. Cheng, andA. M. Seligman: Cytochemical demonstration of succinic dehydrogenase by use of a new p-nitrophenyl substituted diterazole. J. Histochem. Cytochem.5, 420–436 (1957).
—D. G. Walker, andA. Seligman: The histochemical localization of triphosphopyridine nucleotide diaphorase. J. biophys. biochem. Cytol.4, 467–474 (1958).
———: A histochemical demonstration of diphosphopyridine nucleotide diaphorase. J. Histochem. Cytochem.4, 29–38 (1958).
Naidoo, D., andO. E. Pratt: The localization of some phosphatases in brain tissue. J. Neurol. Neurosurg. Psychiat.14, 287–294 (1951).
Padykula, H. A., andE. Herman: Adenosine Triphosphate. Factors affecting the activity of adenosine triphosphatase and other phosphatases as measured by histochemical technique. J. Histochem. Cytochem.3, 161–180 (1955).
Pappenheimer, J. R., S. R. Heisey, andE. F. Jordan: Active transport of diodrast and phenolsulfophhtalein from cerebrospinal fluid to blood. Amer. J. Physiol.200, 1–10 (1961).
Park, C. R.: General aspects of transport phenomena. Membrane Transport and Metabolism, ed. byA. Kleinzeller, andA. Kotyk, p. 19–21. New York: Academic Press 1961.
Post, R. L., andC. D. Albright: Membrane adenosine triphosphatase as a part of a system for active sodium and potassium transport. Membrane Transport and Metabolism, ed. byA. Kleinzeller, andA. Kotyk, p. 219–227. New York: Academic Press 1961.
Scarpelli, D. G., R. Hess, andA. G. E. Pearse: The cytochemical localization of oxidative enzymes. I. DPN diaphorase and TPN diaphorase. J. biophys. biochem. Cytol.4, 747–751 (1958).
Schaltenbrand, G.: Plexus und Meningen. In: Handbuch der Mikroskopischen Anatomie des Menschen, ed. byW. V. Möllendorff, andW. Bargmann, Vol. 4, Part II, p. 80–87. Berlin: Springer 1955.
Skou, J. C.: The relationship of a (Mg2++Na+) activated K+ stimulated enzyme or enzyme system to the active transport of Na+ and K+ across the cell membrane. Membrane Transport and Metabolism, ed.A. Kleinzeller, andA. Kotyk, p. 228–236. New York: Academic Press 1961.
Smith, D. E., E. Streicher, K. Milkovic, andI. Klatzo: Observations on the Transport of Proteins by the Isolated Choroid Plexus (in press).
Stiehler, R. D., andL. B. Flexner: A mechanism of secretion in the choroid plexus. The conversion of oxidation-reduction energy into work. J. biol. Chem.126, 603–617 (1938).
Torrack, R. M., M. Benson, andN. H. Becker: Localization of adenosinetriphosphate in capillaries of the brain as revealed by electronmicroscopy. Neurology (Minneap.)11, 71–76 (1961).
Ussing, H. H., andK. Zerahn: Active transport of sodium as the source of electric current in the most-circulated isolated frog skin. Acta physiol. scand.23, 110–127 (1951).
Wattenberg, L. W., andJ. L. Leong: Effects of Coenzyme Q10 and menadione on succinic dehydrogenase activity as measured by tetrazolium salt reduction. J. Histochem. Cytochem.8, 296–303 (1960).
Welch, K.: Active transport of iodine by choroid plexus of the rabbit in vitro. Amer. J. Physiol.202, 757–760 (1962).
Wislecki, G. B., andE. W. Dempsey: The chemical cytology of the choroid plexus and blood brain barrier of the Rhesus Monkey (Macaca Mulata). J. comp. Neurol.88, 319–345 (1948).
Zerahn, K.: Oxygen consumption and active sodium transport in the isolated and most-activated frog skin. Acta physiol. scand.36, 300–318 (1956).
Author information
Authors and Affiliations
Additional information
Rockefeller Fellow on leave from the Neuropathological Department, Polish Academy of Sciences, Cracow, Poland
With 7 Figures in the Text
Rights and permissions
About this article
Cite this article
Kaluza, J.S., Burstone, M.S. & Klatzo, I. Enzyme histochemistry of the chick choroid plexus. Acta Neuropathol 3, 480–489 (1964). https://doi.org/10.1007/BF00688456
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF00688456