Skip to main content
SpringerLink
Log in

The perineurium as a diffusion barrier to protein tracers

Influence of histamine, serotonine and bradykinine

  • Original Investigations
  • Published:
Acta Neuropathologica Aims and scope Submit manuscript

Summary

A study was made on the influence of histamine, serotonine and bradykinine on the perineurial permeability to horseradish peroxidase in mouse sciatic nerve. Following application of peroxidase around the exposed nerves the drugs were administered locally and the animals were allowed to survive for 5–20 min. The localization of the enzyme was then determined by an enzymehistochemical method.

In control nerves peroxidase diffuses in the epineurium but is prevented from reaching the endoneurium by the impermeable perineurium. Even very high concentrations of the tested substances did not change this type of spread of the marker enzyme. Consequently, the perineurium appears to maintain its property as diffusion barrier to proteins even under the influence of these chemical mediators of the inflammatory process.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Allt, G.: Involvement of the perineurium in experimental allergic neuritis: Electron microscopic observations. Acta neuropath. (Berl.)20, 139–149 (1972)

    Google Scholar 

  • Broman, T., Lindberg-Broman, A. M.: An experimental study of disorders in the permeability of the cerebral vessels (“the blood-brain-barrier”) produced by chemical and physicochemical agents. Acta physiol. scand.10, 102–125 (1945)

    Google Scholar 

  • Burkel, W. E.: The histological fine structure of perineurium. Anat. Rec.158, 177–190 (1967)

    Google Scholar 

  • Cotran, R. S., Litt, M.: Ultrastructural localization of horseradish peroxidase and endogenous peroxidase activity in guinea pig peritoneal macrophages. J. Immunol.105, 1536–1546 (1970)

    Google Scholar 

  • Denny-Brown, P.: Importance of neuronal fibroblasts in the regeneration of nerve. Arch. Neurol. Psychiat. (Chic.)55, 171–215 (1946)

    Google Scholar 

  • Karnovsky, M. J.: A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy. J. Cell Biol.27, 137A (1965)

    Google Scholar 

  • Klemm, H.: Das Perineurium als Diffusionsbarriere gegenüber Peroxydase bei epi- und endoneuraler Applikation. Z. Zellforsch.108, 431–445 (1970)

    Google Scholar 

  • Kristensson, K., Olsson, Y. The perineurium as a diffusion barrier to protein tracers. Differences between mature and immature animals. Acta neuropath. (Berl.)17, 127–138 (1971)

    Google Scholar 

  • Kurucz, J., Osgyáni, J.: Contributions to the pathomorphology of pulmonary innervation. Acta morph. Acad. Sci. hung.4, 227–232 (1954)

    Google Scholar 

  • Majno, G., Palade, G. E.: Studies on inflammation. I. The effect of histamine and serotonine on vascular permeability: An electron microscopic study. J. biophys. biochem. Cytol.11 571–605 (1961)

    Google Scholar 

  • Majno, G., Shea, S. M., Leventhal, M.: Endothelial contraction induced by histamine-type mediators. An electron microscopic study. J. Cell Biol.42, 647–672 (1969)

    Google Scholar 

  • Martin, K. H.: Untersuchungen über die perineurale Diffusionsbarriere an gefriergetrockneten Nerven. Z. Zellforsch.64, 404–428 (1964)

    Google Scholar 

  • Olsson, Y.: Studies on vascular permeability in peripheral nerves: 2. Distribution of circulating fluorescent albumin in rat sciatic nerve after local injection of 5-hydroxytryptamine, histamine and compound 48/80. Acta physiol. scand.69, Supp. 284 (1966)

    Google Scholar 

  • Olsson, Y., Reese, T. S.: Permeability of vasa nervorum and perineurium in mouse sciatic nerve studied by fluorescence and electron microscopy. J. Neuropath. exp. Neurol.30, 105–119 (1971)

    Google Scholar 

  • Olsson, Y., Kristensson, K.: The perineurium as a diffusion barrier to protein tracers following trauma to nerves. Acta neuropath. (Berl.)23, 105–111 (1972)

    Google Scholar 

  • Ross, M. H., Reith, M. J.: Perineurium: Evidence for contractile elements. Science165, 604–606 (1969)

    Google Scholar 

  • Shanta, T. R., Bourne, G. H.: The perineural epithelium — a new concept. In: The structure and function of nervous tissue, Ed. G. H. Bourne, Vol. 1. New York-London: Academic Press 1968

    Google Scholar 

  • Shanthaveerappa, T. R., Bourne, G. H.: The perineural epithelium: Nature and Significance. Nature (Lond.)199, 577–579 (1963)

    Google Scholar 

  • Söderfeldt, B., Olsson, Y. Kristensson, K.: The perineurium as a diffusion barrier to protein tracers in human peripheral nerves. Acta neuropath. (Berl.)25, 120–126 (1973)

    Google Scholar 

  • Zweifach, B. W.: Microcirculatory aspects of tissue injury. Ann. N. Y. Acad. Sci.116, 831–838 (1964)

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Neuropathological Laboratory, Institute of Pathology, University of Uppsala, Box 553, S-75122, Uppsala, Sweden

    Birgitta Söderfeldt

Authors
  1. Birgitta Söderfeldt
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Söderfeldt, B. The perineurium as a diffusion barrier to protein tracers. Acta Neuropathol 27, 55–60 (1974). https://doi.org/10.1007/BF00687240

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00687240

Key words

Search

Navigation