Summary
The fine structure of the glomerular basement membrane (GBM) of the rat kidney was studied by means of high resolution scanning electron microscopy. Specimens were taken from kidneys perfused with paraformaldehyde, freeze-fractured and then processed with conductive staining. The fractured surface of glomerular tufts exhibited the inner and outer surface of the GBM uncovered by endothelial and epithelial cells. The lamina densa was composed of densely packed granular material together with scattered fibrils. The laminae rarae interna and externa were composed of a meshwork that showed some structural heterogeneities. The meshwork composing the lamina rara interna contained 5-to 9-nm-thick fibrils, had pores 11–30 nm wide, and was associated with granular material except in those places that corresponded with endothelial fenestrae. The meshwork of the lamina rara externa was made up of 6- to 11-nm-thick fibrils, and had smaller pores under the foot processes (10–24 nm wide) than those near the filtration slits (16–32 nm wide). In addition to the meshwork, the lamina rara interna contained microfibrils that were arranged differently depending on the topography of the capillary wall: scattered fibrils had no predominant orientation at the convex side, circumferential bundles lay at the concave side of the peripheral capillary wall, and had a circumferential arrangement in the paramesangial wall.
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References
Bohrer MP, Baylis C, Humes HD, Glassock RJ, Robertson CR, Brenner BM (1978) Permselectivity of the glomerular capillary wall. Facililated filtration of circulating polycations. J Clin Invest 61:72–78
Chang RLS, Ueki IF, Troy JL, Deen W, Robertson CR, Brenner BM (1975a) Permselectivity of the glomerular capillary wall to macromolecules. II. Experimental studies in rats using neutral dextran. Biophysical J 15:887–906
Chang RLS, Deen W, Robertson CR, Brenner BM (1975b) Perm-selectivity of the glomerular capillary wall. III. Restricted transport of polyanions. Kidney Int 8:212–218
Desaki J, Uehara Y (1981) The overall morphology of neuromuscular junctions as revealed by scanning electron microscopy. Neurocytol 10:101–110
Farquhar MG (1980) Role of the basement membrane in glomerular filtration: results obtained with electron-dense tracers. In: Maunsbach AB, Olsen TS, Christensen EI (eds) Functional ultrastructure of the kidney. Academic Press, London New York Sydney, pp 31–51
Farquhar MG, Wissig SL, Palade GE (1961) Glomerular permeability. I. Ferritin transfer across the normal glomerular capillary wall. J Exp Med 113:47–66
Gaizutis M, Pesce AJ, Lewy JE (1972) Determination of nanogram amounts of albumin by radioimmunoassay. Microchem J 17:327–337
Glauert AM (1965) Factors influencing the appearance of biologic specimens in negative stained preparations. Lab Invest 14:1069–1079
Hijikata T, Sakai T (1991) Structural heterogeneity of the basement membrane in the rat proximal tubule. Cell Tissue Res 266:11–22
Kubosawa H, Kondo Y, (1985) Ultrastructural organization of the glomerular basement membrane as revealed by a deep-etch replica method. Cell Tissue Res 242:33–39
Latta H (1970) The glomerular capillary wall. J Ultrastruct Res 32:526–544
Laurie GW, Leblond CP, Inoue S, Martin GR, Chung A (1984) Fine structure of the glomerular basement membrane and immunolocalization of five basement membrane components to the lamina densa (basal lamina) and its extensions in both glomeruli and tubules of the rat kidney. Am J Anat 169:463–481
Leblond CP, Inoue S (1989) Structure, composition, and assembly of basement membrane. Am J Anat 185:367–390
Martinez-Hernandez A (1978) The basement membrane pores. In: Kefalides NA (ed) Biology and chemistry of basement membranes. Academic Press, New York San Francisco London, pp 99–109
Mundel P, Elger M, Sakai T, Kriz W (1988) Microfibrils are major component of the mesangial matrix in the glomerulus of the rat kidney. Cell Tissue Res 254:183–187
Murakami T (1971) Application of the scanning electron microscope to the study of the fine distribution of the blood vessels. Arch Histol Jpn 32:445–454
Murakami T (1973) A metal impregnation method of biological specimens for scanning electron microscopy. Arch Histol Jpn 35:323–326
Orkin RW, Gehron P, McGoon EB, Martin GR, Valentine T, Swarm R (1977) A murine tumor producing a matrix of basement membrane. J Exp Med 145:204–220
Osumi M, Yamada N, Kobori H, Taki A, Naito N, Baba M, Nagatani T (1989) Cell wall formation in regenerating protoplasts ofSchizosaccharomyces pombe: study by high resolution, low voltage scanning electron microscoy. J Electron Microsc 38:457–468
Ota Z, Makino H, Miyoshi A, Hiramatu M, Takahashi K, Ofuji T (1979) Molecular sieve in glomerular basement membrane as revealed by electron microscopy. J Electron Microsc 28:20–28
Rodewald R, Karnovsky M (1974) Porous substructure of the glumerular slit diaphragm in the rat and mouse. J Cell Biol 60:423–433
Sakai T, Kriz W (1987) The structural relationship between mesangial cells and basement membrane of the renal glomerulus. Anat Embryol 176:373–386
Sakai T, Sabanovic S, Hosser H, Kriz W (1986) Heterogeneity of the podocyte membrane in the rat kidney as revealed by ethanol dehydration of unosmicated specimens. Cell Tissue Res 246:145–151
Takahashi-Iwanaga H, Iwata Y, Adachi K, Fujita T (1989) The histography and ultrastructure of the thin limb of the Henle's loop: a scanning electron microscopic study of the rat kidney. Arch Histol Cytol 52:395–405
Tanaka K, Naguro T (1981) High resolution scanning electron microscopy of cell organelles by a new specimen preparation method. Biomed Res 2 [Suppl]:63–70
Timpl R, Fujiwara S, Dziadek M, Aumailley M, Weber S, Engel J (1984) Laminin, proteoglycan, nidogen and collagen IV: structural models and molecular interactions. In: Porter R, Whelan J (eds) Basement membrane and cell movement. Chiba Foundation Symposium 108, Pitman, London, pp 25–43
Yurchenco PD, Tsilibary EC, Charonis AS, Furthmayer H (1986) Models for the self-assembly of basement membrane. J Histochem Cytochem 34:93–102
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Shirato, I., Tomino, Y., Koide, H. et al. Fine structure of the glomerular basement membrane of the rat kidney visualized by high-resolution scanning electron microscopy. Cell Tissue Res. 266, 1–10 (1991). https://doi.org/10.1007/BF00678705
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DOI: https://doi.org/10.1007/BF00678705