Summary
During sulfate reduction in a cell-free system ofChlorella activated sulfate of APS is transferred to a thiosulfonate reductase. The sulfate thus bound to the thiosulfonate reductase (i.e. bound sulfite) is reduced to bound sulfide in a ferredoxin dependent reaction. This bound sulfide can be used with O-acetylserine as acceptor for cysteine biosynthesis; serine and O-phosphoserine are not used. An assaysystem for thiosulfonate reductase activity using methylviologen dependent reduction of S2O4 2− to S2− is developed and a procedure for isolating thiosulfonate reductase fromChlorella cells is presented. During isolation of thiosulfonate reductase a low weight molecular factor, needed for optimal enzyme activity was lost. The bound sulfite seems to be attached to this factor. Reduction of APS or GS-SO3H to the level of S2− is inhibited by cysteine. 50% inhibition of GS-SO3H reduction was found at a molar cysteine concentration of 6.8×10−5.
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Abbreviations
- APS:
-
adenosine-phosphosulfate
- PAPS:
-
3′-phosphoadenosine-5′-phosphosulfate
- GSH:
-
reduced glutathion
- GS-SO3H:
-
S-sulfoglutathion
- fd:
-
ferredoxin
- Mv:
-
methylviologen
- DTT:
-
dithiothreitol
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