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Study of polysaccharides by the fluorescence method. V. Interaction of 2-p-toluidinylnaphthalene-6-sulfonate with amylose and its related compounds in aqueous solution

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Abstract

The interaction of 2-p-toluidinylnaphthalene-6-sulfonate (TNS) with amylose and its related compounds in aqueous solution has been studied by both steady-state and transient fluorescence measurements. The fluorescence of TNS aqueous solution was enhanced by the addition of amylose, β-limit dextrin, and amylopectin. The fluorescence decay of TNS bound to these polysaccharides were well described as a sum of two-exponential functions. This suggests that there are two different microenvironments at the binding sites. The fluorescence lifetime of major component for TNS-amylose system agreed with that of major component for TNS-γ-cyclodextrin system. The mean rotational relaxation time of TNS bound to amylose is similar to that of the segmental motion of amylose chain. Based on these results, a configurational model for TNS-amylose complex has been proposed.

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Authors and Affiliations

  1. Department of Agricultural Chemistry, Kyoto Prefectural University, Shimogamo, Sakyo-ku, 606, Kyoto, Japan

    Shinichi Kitamura, Shigeru Matsumori & Takashi Kuge

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  1. Shinichi Kitamura
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  2. Shigeru Matsumori
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  3. Takashi Kuge
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Kitamura, S., Matsumori, S. & Kuge, T. Study of polysaccharides by the fluorescence method. V. Interaction of 2-p-toluidinylnaphthalene-6-sulfonate with amylose and its related compounds in aqueous solution. Journal of Inclusion Phenomena 2, 725–735 (1984). https://doi.org/10.1007/BF00662240

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