Summary
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1.
The effects of temperatures between 10–32 °C on the slowly adapting stretch receptor neuron of the crayfish were studied with intracellular recording and potential clamp techniques.
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2.
The membrane potential was found to decrease about 0.3 mV/ °C with increasing temperature in the range of 10° to 30 °C.
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3.
At temperatures in the range of 25° to 32 °C stretch produced a brief intense impulse discharge followed by a maintained depolarization. A similar response could be elicited by intracellular cathodal pulses or appeared sometimes spontaneously.
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4.
Tetrodotoxin (TTX) at a concentration of 10−7–10−8 mg/ml completely abolished the plateau, the receptor potential remaining unaffected.
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5.
Lowering of Ca+ + concentration facilitated the development of the plateau while increase of Ca+ + concentration had the opposite effect.
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6.
At a resting potential of −68 mV and at +30 °C the pNa/pK was 0.03 using the Goldman equation, the PNa/PK at a plateau level of −35 mV being 0.18. —The time constant for the inactivation of the plateau response was in the order of 1 s.
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7.
The passive membrane conductance as measured with the potential clamp technique increased with increasing temperature.
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8.
The stretch induced current (SIC) decreased and the reversal potential (E rev) was shifted towards more positive values when temperature was raised.
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9.
It is suggested that the steplike shift of membrane potential at high temperatures may be attributed to an increase in PNa/PK of the regenerative portion of the membrane and that this increase as well as the increase in passive membrane conductance are accounted for by changes in the physical properties of the lipid phase of the membrane at high temperatures.
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Abbreviations
- E rev :
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reversal potential
- SIC :
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Stretch induced current
- TTX :
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Tetrodotoxin
References
Blok, M.C., Van Der Neut-Kok, E.C.M., Van Deenen, L.L.M., De Gier, J.: The effects of chain length and lipid phase transitions on the selective permeability properties of liposomes. Biochem. Biophys. Acta406, 187–196 (1975)
Blok, M.C., Van Deenen, L.L.M., De Gier, J.: Effect of the gel to liquid crystalline phase transition on the osmotic behaviour of phosphotidylcholine liposomes. Biochem. Biophys. Acta433, 1–12 (1976)
Brown, H.M., Ottoson, D., Rydqvist, B.: Crayfish stretch receptor: An investigation with voltage-clamp and ion-sensitive electrodes. J. Physiol. London284, 155–179 (1978)
Chapman, D., Williams, R.M., Ladbrooke, B.D.: Physical studies of phospholipids. VI. Thermotropic and lyotropic mesomorphin of some 1.2-diacyl-phosphatidylcholine (lecithins). Chem. Phys. Lipids1, 445–475 (1967)
Edwards, C., Terzuolo, C.A., Washizu, Y.: The effect of changes of the ionic environment upon an isolated crustacean sensory neuron. J. Neurophysiol.26, 948–957 (1963)
Eyzaguirre, C., Kuffler, S.W.: Processes of excitation in the dendrites and in the soma of single isolated sensory nerve cells of the lobster and crayfish. J. Gen. Physiol.20, 87–119 (1955)
Fitzhugh, R.: Impulses and physiological states in theoretical models of nerve membrane. Biophys. J.1, 445–466 (1961)
Frankenhaeuser, B.: Quantitative description of sodium currents in myelinated nerve fibres ofXenopus laevis. J. Physiol. London151, 491–501 (1960)
Frankenhaeuser, B.: A quantitative description of potassium currents in myelinated nerve fibres ofXenopus laevis. J. Physiol. London169, 424–430 (1963)
Frankenhaeuser, B., Hodgkin, A.L.: The action of calcium on the electrical properties of squid axons. J. Physiol. London137, 218–244 (1957)
Frankenhaeuser, B., Moore, L.E.: The effect of temperature on the sodium and potassium permeability changes in myelinated nerve fibres ofXenopus laevis. J. Physiol. London169, 431–437 (1963)
Goldman, D.E: Potential, impedance and rectification in membranes. J. Gen. Physiol.27, 37–60 (1943)
Grampp, W.: Multiple-spike discharge evoking after-depolarizations in the slowly adapting stretch receptor neuron of the lobster. II. The slow after-depolarization. Acta Physiol. Scand.67, 116–126 (1966)
Harreveld, A. Van: A physiological solution for freshwater crustaceans. Proc. Soc. Exp. Biol.34, 428–432 (1936)
Harri, M., Florey, E.: The effects of temperature on a neuromuscular system of the crayfish,Astacus leptodactylus. J. Comp. Physiol.117, 47–61 (1977)
Hodgkin, A.L., Katz, B.: The effect of sodium ions on the electrical activity of the giant axon of the squid. J. Physiol. London108, 37–77 (1949)
Ottoson, D., Rydqvist, B.: The effects of Triton-detergents on the stretch receptor of the crayfish. Acta Physiol. Scand.103, 9–18 (1978)
Teorell, T.: A biophysical analysis of mechano-electrical transduction. In: Handbook of sensory physiology, Vol. 1. Loewenstein, W.R. (ed.), pp. 291–339. Berlin, Heidelberg, New York: Springer 1971
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We are greatly indebted to Prof. C. Edwards for valuable criticism and suggestions during the preparation of the manuscript. The technical assistance of Mrs M. Hohn is greatfully acknowledged. This investigation has been supported by grants from the Swedish Medical Research Council, project no B77-14X-0043-13B-14C-15, and the Fond zur Förderung der wissenschaftlichen Forschung in (Jsterreich, project no 3315. The work was made possible also by a Stipendium from the Swedish Institute to H. Moser.
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Moser, H., Ottoson, D. & Rydqvist, B. Step-like shifts of membrane potential in the stretch receptor neuron of the crayfish (Astacus fluviatilis) at high temperatures. J. Comp. Physiol. 133, 257–265 (1979). https://doi.org/10.1007/BF00661128
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DOI: https://doi.org/10.1007/BF00661128