Abstract
A new method for providing polymeric microspheres with aldehyde functional groups capable of covalent reaction with proteins is described. Benzyl halide groups on small molecules, polymeric resins, and the surfaces of surfactant-free poly(styrene-co-chloromethylstyrene) were converted to benzaldehyde groups by oxidation with 2-nitropropane in aqueous sodium methoxide. The modified microspheres containing aldehyde groups can be reacted with protein under milder conditions than the parent microspheres. The activity of a monoclonal antibody, Phe 1.9, was higher when it was immobilized on the aldehyde-containing beads than following immobilization on the parent beads.
Similar content being viewed by others
References
Rembaum A, Dreyer WJ (1980) Science 208:364–8
Margel S (1983) Applied Biochem Biotechnol 8:523
Pines M, Margel S (1986) J Immunoassay 7:97
Kreuter J (1988) Microencapsutation 5(2):115
Yen SPS, Rembaum A, Molday RW, Dreyer W (1976) ACS Symp Ser 236-257
Rembaum A, Yen SPS (1979) J Macromol Sci Chem A 13(5):603–632
Ugelstad J, Soderberg L, Berge A, Bergstrom J (1983) Nature 303:5
Margel S. Rembaum A (1980) Macromolecules 13:19
Rembaum A, Chang M, Richards G, Li M (1984) J Polym Sci Polym Chem Ed 22:609–621
Rembaum A, Yen RCK, Kempner DH, Ugelstad J (1982) J Immunol Methods 52:341–351
Huang Z, Huang X (1986) Huaxue Shiji 8(1):18–19, CA 105(17), 1522h
Gopalan R, Subbarayan K (1979) J Ind Chem Soc 56(7):669–72
Kumpf W, Martinez D (1984) Z Chem 24(5):182–3
Igarashi T, Toyota M, Suzuki M (1980) Kanagowa Daigaku Kogakubu Kenkyo Hokoko 18:72–6
Igarashi T, Sakurai M, Suzuki M (1979) Kanagowa Daigaku Kogakubu Kenkyo Hokoko 17:34–8
Ferraboschi P, Azandi MN, Santaniello E, Trave S (1986) Synth Commun 16:43–45
Mukaiyama S, Inanaga J, Yamaguchi M (1981) Bull Chem Soc Jpn 54(7)2221–2
Bernhard G, Petersen EN, Daum G (4 June, 1981) Ger Offen DE 2948058
Ayres JT, Mann CK (1965) Polym Lett 3:505–8
Syper L, Mlochowski J (1984) Synthesis 9:747–52
Gibson HW, Bailey FC (1975) J Polym Sci Polym Chem Edition 13:1951–5
Haar HB, Bender ML (1949) J Am Chem Soc 71:1767–9
Haas HB, Bender ML (1949) J Am Chem Soc 71:3482–5
Kerber RC, Urry GW, Kornblum N (1965) J Am Chem Soc 87:4520–8
Kerber RC, Urry GW, Kornblum N (1964) J Am Chem Soc 86:3904–5
Warshawsky A, Deshe A, Gutman R (1984) Brit Polym J 16:234
Bale MD, Danielson SJ, Daiss JL, Goppert KE, Sutton RC (1989) J Colloid Interface Sci 132:176–187
Mosher DF, Vaheri A (1978) Exp Cell Res 112:323–334
Bale MD, Mosher DF, Wolfarht L, Sutton RC (1988) J Colloid Interface Sci 125:516–525
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bale Oenick, M.D., Warshawsky, A. Protein immobilization on surface modified latices bearing aldehyde groups. Colloid Polym Sci 269, 139–145 (1991). https://doi.org/10.1007/BF00660303
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00660303