Abstract
The effects of salinity on the lateral headgroup interactions of dipalmitoylphosphatidylcholine (DPPC) molecules in fully hydrated multilayers have been investigated by spin label electron spin resonance (ESR) spectroscopy and differential scanning calorimetry (DSC).
By increasing the NaCl concentration from 0 to 3 M in the multilayers' dispersion medium, the ESR measurements performed with the 5-stearic acid spin label and di-tert-butyl-nitroxide show an increase in the orientational degree of order of the lipid molecules, mainly in the gel phase, and a decrease of the membrane permeability. An upward shift from 31.5° to 36.5°C and from 40.5° to 41.9°C of the pre- and main DPPC phase transition temperatures, respectively, is observed with 5-SASL, while slightly higher values are detected with DTBN. Small effects are evident on the properties of the liquid crystalline phase of the DPPC multilayers.
The DSC measurements also reveal an upward shift of the pre- and main transition temperatures. The shifts, however, are more marked if compared to the ones observed with the ESR technique.
The findings suggest an increase in the packing density of the DPPC molecules in the multilayers in presence of high salt concentration. Dehydration of the DPPC interfacial region with a variation of the lateral electrostatic interactions between phospholipid polar heads trigger the phenomena observed.
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Sapia, P., Coppola, L., Ranieri, G. et al. Effects of high electrolyte concentration on DPPC-multilayers: an ESR and DSC investigation. Colloid Polym Sci 272, 1289–1294 (1994). https://doi.org/10.1007/BF00657783
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DOI: https://doi.org/10.1007/BF00657783