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The Effect of Divalent Ions on the Structure of Bilayers in the Dimyristoylphosphatidylcholine Vesicles

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Abstract

We have studied changes in the structural parameters of 1,2-dimyristoyl-sn-3-phosphocholine (DMPC) unilamellar vesicles at a concentration series 0–30 mM of divalent metal cations Ca2+, Mg2+, and Co2+ by means of small-angle neutron scattering (SANS). The membrane structural parameters (thickness and area per lipid) were obtained at different concentrations of cations in the gel and fluid phases of membrane. Both Ca2+ and Mg2+ ions at the concentrations of 0–1 mM increase the membrane thickness by 1.9 Å and 2.9 Å in the fluid and gel phase, respectively. In the concentration range of 1–30 mM, either a weak tendency to a thickness decrease of ~ 1 Å is observed, or the thickness does not change at all. In the case of Co2+ ions, all changes are extremely weak. We advocate a model of electrostatic interactions for these systems that encompasses the formation of ion bridges between lipid headgroups. Using the Langmuir adsorption isotherm we estimate the fraction of Ca2+ ions bound to the DMPC membrane. The developed model is of an interest to the future studies of membrane interactions with various charged peptides, such as those from the amyloid-β family.

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Funding

This study was supported by the Russian Science Foundation (grant no. 19-72-20 186).

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Authors and Affiliations

  1. Institute of Physics, Kazan Federal University, 420008, Kazan, Russia

    S. A. Kurakin

  2. Frank Laboratory of Neutron Physics, Joint Institute for Nuclear Research, 141980, Dubna, Russia

    S. A. Kurakin, E. V. Ermakova, A. I. Ivankov & N. Kučerka

  3. Moscow Institute of Physics and Technology, 141701, Dolgoprudnyi, Russia

    A. I. Ivankov

  4. Institute for Safety Problems of Nuclear Power Plants, National Academy of Sciences of Ukraine, 03028, Kyiv, Ukraine

    A. I. Ivankov

  5. Kazan National Research Technological University, 420015, Kazan, Russia

    S. G. Smerdova

  6. Department of Physical Chemistry of Drugs, Faculty of Pharmacy, Comenius University in Bratislava, 832 32, Bratislava, Slovakia

    N. Kučerka

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  1. S. A. Kurakin
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Correspondence to S. A. Kurakin or N. Kučerka.

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Translated by O. Kadkin

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Kurakin, S.A., Ermakova, E.V., Ivankov, A.I. et al. The Effect of Divalent Ions on the Structure of Bilayers in the Dimyristoylphosphatidylcholine Vesicles. J. Surf. Investig. 15, 211–220 (2021). https://doi.org/10.1134/S1027451021020075

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