Abstract
The ion-exchange reaction of lysozyme with carboxymethyl Sephadex C-25 was followed by conductivity change as a function of time just after the rapid mixing of the protein solution with the Sephadex suspension. A single relaxation process was observed; the conductivity increased exponentially with time in the 100 s scale. In this process, protons were released from the Sephadex C-25 in the same time scale. The relaxation process slowed down with an increase in the lysozyme concentration, but it quickened upon the addition of HCl. On the other hand, the ζ potential on the Sephadex C-25 surface changed from a negative value to a positive one with an increase in the amount of lysozyme adsorbed on the surface. On the basis of these data, the relaxation process was attributed to the ion-exchange reaction of lysozyme with several protons of carboxymethyl groups of the Sephadex.
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References
Peterson EA, Sobers HA (1956) J Am Chem Soc 78:751
Bendich A, Pahl HB, Korngold GC, Rosenkranz S, Fresco JR (1958) J Am Chem Soc 80:3949
Heide K, Haupt H, Schwick HG (1977) In: Putnum FW (ed) The Plasma Protein, Academic Press, New York, 2nd ed, 4:545
Janson JC, Hedman P (1982) Adv Biochem Eng 25:43
Wang SC, Tien C (1982) AIChE J 28:565
Hachiya K, Sasaki M, Nabeshima Y, Mikami N, Yasunaga T (1984) J Phys Chem 88:1623
Sasaki M, Hachiya K, Ikeda T, Yasunaga T (1984) J Phys Chem 88:1627
Hachiya K, Takeda K, Yasunaga T (1987) Adsorpt Sci Technol 4:25
Hachiya K, Yasunaga T, Takeda K (1989) Colloids Surfaces 37:205
Graham EE, Fook CF (1982) AIChE J 28:245
Tsou H-S, Graham EE (1985) AIChE J 31:1959
Skidmore GL, Horstmann BJ, Chase HA (1990) J Chromatgr 498:113
Hammes GG (1974) In: Hammes GC (ed) Technique of Chemistry Vol. VI, part 2. Willey-Interscience, New York p 147
Saxena VP, Wetlaufer DB (1970) Biochemistry 9:5015
Davis JA, James RO, Leckie JO (1978) J Colloid Intertace Sci 63:480
Hachiya K, Yamamoto K, Inoue T, Takeda K (1992) J Colloid Interface Sci 150:270
Whitley RD, Wachter R, Liu F, Wang N-HL (1989) J Chromatogr 465:137
Phillips DC (1966) Sci American 215:78
Bernasconi CF (1976) Relaxation Kinetics, Academic Press, New York, 11
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Yamamoto, K., Hachiya, K. & Takeda, K. Kinetic study of ion exchange reaction between lysozyme and carboxymethyl Sephadex C-25. Colloid Polym Sci 270, 878–884 (1992). https://doi.org/10.1007/BF00657732
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DOI: https://doi.org/10.1007/BF00657732