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Effect of light on ATPases in eyes and brain of the blowfly,Calliphora

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Summary

The relation between ATPase-activities and stimulation of a compound eye by light was investigated biochemically: (1) The Mg2+- and Na+K+-ATPases of eyes with different amounts of screening pigments were determined in flies reared under identical light conditions. (2) Enzyme activities in eyes were compared after dark adaptation and illumination, respectively. (3) Activity measurements were also taken from the brains of the same animals.—The ATPase activities-measured in vitro under optimal conditions —remained unchanged under illumination of medium intensity. After high level illumination of a pigment-free mutant, Na+K+-ATPase (but not Mg2+-ATPase) activity raised significantly in eyes as well as in brain (Table 3).—In vivo only activity of the membrane-bound Na+K+-ATPase changes with illumination, but the amount of enzyme protein is large enough that sub-maximal turnover rates occur under usual light conditions. High intensity light causes synthesis of new enzyme protein in pigment-free eyes.

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Authors and Affiliations

  1. Institut für Tierphysiologie der Ruhr-Universität Bochum, Postfach 1021 48, D-4630, Bochum 1, Federal Republic of Germany

    Maria E. Rivera & Helmut Langer

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  1. Maria E. Rivera
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  2. Helmut Langer
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This investigation was supported by Deutsche Forschungsgemeinschaft, Sonderforschungsbereich 114 (“Bionach”)

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Rivera, M.E., Langer, H. Effect of light on ATPases in eyes and brain of the blowfly,Calliphora . J. Comp. Physiol. 123, 245–251 (1978). https://doi.org/10.1007/BF00656877

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  • DOI: https://doi.org/10.1007/BF00656877

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