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A new method for obtaining isolated photoreceptors from the amphibian retina

  • Excitable Tissues and Central Nervous Physiology
  • Instruments and Techniques
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Abstract

A new method is described for obtaining isolated rods and cones by physical dissociation of the retina. The method gives a large yield of isolated photoreceptors, without any changes in membrane properties that enzymatic dissociation techniques might produce. Photovoltages and photocurrents recorded from rods isolated in this way are presented.

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References

  • Attwell D, Werblin FS, Wilson M (1982) The properties of single cones isolated from the tiger salamander retina. J Physiol Lond 328:259–283

    Google Scholar 

  • Attwell D, Wilson M (1980) Behaviour of the rod network in the tiger salamander retina mediated by membrane properties of individual rods. J Physiol Lond 309:287–315

    Google Scholar 

  • Bader CR, MacLeish PR, Schwartz EA (1978) Responses to light of solitary rod photoreceptors isolated from the tiger salamander retina. Proc Natl Acad Sci USA 75:3507–3511

    Google Scholar 

  • Bader CR, MacLeish PR, Schwartz EA (1979) A voltage-clamp study of the light response in solitary rods of the tiger salamander. J Physiol Lond 296:1–26

    Google Scholar 

  • Bader CR, Bertrand D, Schwartz EA (1982) Voltage-activated and calcium-activated currents studied in solitary rod inner segments from the salamander retina. J Physiol Lond 331:253–284

    Google Scholar 

  • Baylor DA, Fuortes MGF, O'Bryan PM (1971) Receptive fields of cones in the retina of the turtle. J Physiol Lond 214:265–294

    Google Scholar 

  • Baylor DA, Lamb TD, Yau KW (1979) The membrane current of single rod outer segments. J Physiol Lond 288:589–611

    Google Scholar 

  • Schwartz EA (1975) Cones excite rods in the retina of the turtle. J Physiol Lond 246:639–651

    Google Scholar 

  • Schwartz EA (1976) Electrical properties of the rod syncytium in the retina of the turtle. J Physiol Lond 257:379–406

    Google Scholar 

  • Werblin FS (1978) Transmission along and between rods in the tiger salamander retina. J Physiol Lond 280:449–470

    Google Scholar 

  • Yau KW, McNaughton PA, Hodgkin AL (1981) Effect of ions on the light-sensitive current in retinal rods. Nature, Lond 292: 502–505

    Google Scholar 

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Authors and Affiliations

  1. Department of Zoology, University of California, 95616, Davis, CA, USA

    Martin Wilson

  2. Department of Physiology, University College London, Gower Street, WC1E 6BT, London, UK

    David Attwell

Authors
  1. Martin Wilson
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  2. David Attwell
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Wilson, M., Attwell, D. A new method for obtaining isolated photoreceptors from the amphibian retina. Pflugers Arch. 399, 238–240 (1983). https://doi.org/10.1007/BF00656722

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  • DOI: https://doi.org/10.1007/BF00656722

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