Summary
Canine saphenous veins were either placed in Krebs-Henseleit solution and stored for 24 h at +4°C, or immersed in FCS (fetal calf serum) containing 1.8 mol/l DMSO (dimethyl sulfoxide), slowly frozen to-70°C and stored for 4 weeks at-70°C or-190°C. Canine basilar arteries were either stored in Krebs-Henseleit solution for 24 h at +4°C or slowly frozen and stored for 3 months in FCS plus 1.8 mol/l DMSO at-70°C. Subsequent pharmacological investigations revealed a considerable attenuation of the contractile force of frozen-stored vessels but the evidence suggests that there may be a very good preservation of the main biochemical properties, such as monoamine oxidase activity, endogenous prostaglandin synthesis and uptake1 mechanisms in veins stored at-190°C and there is an excellent correlation of thepD2 values for various tryptamine derivatives on canine basilar arteries stored for 3 months at-70°C with those calculated on fresh preparations. It is concluded that freezing isolated blood vessels may be considered an effective means of preserving and storing vascular tissues for pharmacological investigations.
Similar content being viewed by others
References
Balderman SC, Montes M, Schwartz K, Hart T, Bhayana JN (1984) Preparation of venous allografts. A comparison of techniques. Ann Surgery (Philadelphia) 200:117–130
Boren CH, Roon AJ, Moore WS (1978) Maintenance of viable arterial allografts by cryopreservation. Surgery 83:382–391
Dent TL, Weber TR, Lindenauer SM, Ascher N, Weatherbee L, Allen E, Spencer HH (1974) Cryopreservation of vein grafts. Surg Forum (Philadelphia) 25:241–243
Elliott K, Whelan J (1977) The freezing of mammalian embryos. Ciba Foundation Symposium 52, North-Holland
Farrant J (1964) Pharmacological actions and toxicity of dimethyl sulfoxide and other compounds which protect smooth muscle during freezing and thawing. J Pharm Pharmacol 16:472–483
Haudenschild CC, Gould KE, Quist WC, Logerfo FW (1981) Protection of endothelium in vessel segments excised for grafting. Circulation 46 (Suppl II): II/102-II/107
Jackson CV, Karow AM, Carrier GO (1979) Influence of dimethyl sulfoxide (Me2SO) on vascular smooth muscle. Arch Int Pharmacodyn 237:4–15
L'Italien GJ, Maloney RD, Abbott WM (1979) The preservation of the mechanical properties of venous allografts by freezing. J Surg Res 27:239–243
Litvan GG (1972) Mechanism of cryoinjury in biological systems. Cryobiology 9:182–189
Malone JM, Moore WS, Kischer CW, Keown K, Conine R (1980) Venous cryopreservation: Endothelial fibrinolytic activity and histology. J Surg Res 29:209–222
Mazur P (1977) Slow freezing injury in mammalian cells. In: Elliot K, Whelan J (eds) The freezing of mammalian embryos. Elsevier, North Holland, pp 19–42
Müller-Schweinitzer E, Engel G (1983) Evidence for mediation by 5-HT2 receptors of 5-hydroxytryptamine-induced contraction of canine basilar artery. Naunyn-Schmiedeberg's Arch Pharmacol 324:287–292
Spilker B (1972) Pharmacological studies on dimethyl sulfoxide. Arch Int Pharmacodyn 200:153–167
Stanley JC, Sottiurai V, Fry RE, Fry WJ (1975) Comparative evaluation of vein graft preparation media: Electron and light microscope studies. J Surg Res 18:235–246
Vane JR (1959) The relative activities of some tryptamine analogues on the isolated rat stomach preparation. Br J Pharmacol 14:87–98
Weber TR, Dent DL, Lindenauer SM, Allen E, Weatherbee L, Spencer HH, Gleich P (1975) Viable vein graft preservation. J Surg Res 18:247–255
Weber TR, Lindenauer SM, Dent TL, Allen E, Salles CA, Weatherbee L (1976) Long-term patency of vein grafts preserved in liquid nitrogen in dimethylsulfoxide. Ann Surg 184:709–712
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Müller-Schweinitzer, E., Tapparelli, C. Pharmacological studies on frozen stored canine saphenous veins and basilar arteries. Naunyn-Schmiedeberg's Arch. Pharmacol. 332, 74–78 (1986). https://doi.org/10.1007/BF00633201
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00633201