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Techniques for the demonstration of myoglobin in biological materials

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Summary

Paper or agar gel electrophoresis is a simple and convenient method for the demonstration of the presence of Mb in a kidney extract or biological fluids.

TLC on Sephadex may be used for the separation of Mb and Hb as well. Immunoelectrophoresis can serve for the detection of minute quantities of Mb in vivo.

Zusammenfassung

Papiero- der Agargel-Elektrophorese ist eine einfache und gebräuchliche Methode für den Nachweis von Myoglobin im Nierenextrakt oder in biologischen Flüssigkeiten.

TCL (Dünnschichtchromatographie) auf Sephadex kann ebenso gut für die Trennung von Myoglobin und Hämoglobin verwendet werden. Immunelektrophorese kann für die Entdeckung von Myoglobinspuren in vivo dienen.

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Authors and Affiliations

  1. Department of Chemistry and Biochemistry and Department of Forensic Medicine, Medical Fakulty, Charles University, Hradec Králové

    Z. Stránský & M. Srch

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  1. Z. Stránský
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  2. M. Srch
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Stránský, Z., Srch, M. Techniques for the demonstration of myoglobin in biological materials. Dtsch. Z. ges. gerichtl. Med. 66, 123–129 (1969). https://doi.org/10.1007/BF00627791

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