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Introduction of sense and antisense cDNA for branching enzyme in the amylose-free potato mutant leads to physico-chemical changes in the starch

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Abstract

One isoform of the branching enzyme (BE; EC 2.4.1.18) of potato (Solarium tuberosum L.) is known and catalyses the formation of α-1,6 bonds in a glucan chain, resulting in the branched starch component amylopectin. Constructs containing the antisense or sense-orientated distal 1.5-kb part of a cDNA for potato BE were used to transform the amylose-free (amf) mutant of potato, the starch of which stains red with iodine. The expression of the endogenous BE gene was inhibited either largely or fully as judged by the decrease or absence of the BE mRNA and protein. This resulted in a low percentage of starch granules with a small blue core and large red outer layer. There was no effect on the amylose content, degree of branching or λmax of the iodine-stained starch. However, when the physico-chemical properties of the different starch suspensions were assessed, differences were observed, which although small indicated that starch in the transformants was different from that of theamf mutant.

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Abbreviations

amf :

amylose-free

BE:

branching enzyme

G′ :

storage modulus

λmax :

wavelength with maximal absorption of iodine-stained starch

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Authors and Affiliations

  1. Graduate School of Experimental Plant Sciences, Department of Plant Breeding, Agricultural University, P.O. Box 386, 6700 AJ, Wageningen, The Netherlands

    E. Flipse, L. Suurs, E. Jacobsen & R. G. F. Visser

  2. Department of Food Science, Agricultural University, P.O. Box 8129, 6700 EV, Wageningen, The Netherlands

    C. J. A. M. Keetels

  3. Institute für Genbiologische Forschung Berlin GmbH, Ihnestrasse 63, D-14195, Berlin, Germany

    J. Kossmann

Authors
  1. E. Flipse
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  2. L. Suurs
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  3. C. J. A. M. Keetels
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  4. J. Kossmann
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  5. E. Jacobsen
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  6. R. G. F. Visser
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Corresponding author

Correspondence to R. G. F. Visser.

Additional information

We wish to thank the following persons from the department of Plant Breeding, Agricultural University, Wageningen, The Netherlands: T. van de Brink, D.J. Huigen, J. Rijkse, I. Straatman and BJ. de Vries for technical assistance and Dr. M.S. Ramanna for critically reading this manuscript.

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Flipse, E., Suurs, L., Keetels, C.J.A.M. et al. Introduction of sense and antisense cDNA for branching enzyme in the amylose-free potato mutant leads to physico-chemical changes in the starch. Planta 198, 340–347 (1996). https://doi.org/10.1007/BF00620049

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  • DOI: https://doi.org/10.1007/BF00620049

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