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The receptor potential of retinular cells of the blowflyCalliphora: The role of sodium, potassium and calcium ions

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Summary

Simultaneous membrane potential and membrane resistance measurements and current clamp experiments were performed on the visual sense cells ofCalliphora. The results suggest that the receptor potential is due to two different conductance variations: (i) a conductance increase for both Na+- and K+-ions upon illumination, and (ii) a light-independent conductance increase for K+-ions. Evidence is obtained that in the visual sense cells ofCalliphora the light-independent conductance increase is mediated by the influx of Ca2+-ions.

The conclusion is that the increase of the intracellular Ca2+-ion concentration not only decreases the light-induced conductance, but also increases the light-independent conductance. So the potential of the sense cell is governed by two antagonistic conductances. The functional advantages of such a system are discussed.

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References

  • Bader, C.R., Baumann, F., Bertrand, D.: Role of intracellular calcium and sodium in light adaptation in the retina of the honeybee drone (Apis mellifera L.) J. Gen. Physiol.67, 475–491 (1976)

    Google Scholar 

  • Baylor, D.A., Fuortes, M.G.F., O'Bryan, P.M.: Receptive fields of single cones in the retina of the turtle. J. Physiol. (Lond.)214, 265–294 (1971)

    Google Scholar 

  • Brown, H.M.: Intracellular Na+, K+ and Cl− activities inBalanus photoreceptors. J. Gen. Physiol.68, 281–296 (1976)

    Google Scholar 

  • Brown, J.E., Blinks, J.R.: Changes in intracellular free calcium concentration during illumination of invertebrate photoreceptors. Detection with aequorin. J. Gen. Physiol.64, 643–665 (1974)

    Google Scholar 

  • Brown, H.M., Hagiwara, S., Koike, H., Meech, R.M.: Membrane properties of a barnacle photoreceptor examined by the voltage clamp technique J. Physiol. (Lond.)208, 385–413 (1970)

    Google Scholar 

  • Brown, J.E., Lisman, J.E.: An electrogenic sodium pump inLimulus ventral photoreceptor cells. J. Gen. Physiol.59, 720–733 (1972)

    Google Scholar 

  • Brown, J.E., Lisman, J.E.: Intracellular Ca modulates sensitivity and time scale inLimulus ventral photoreceptors. Nature258, 252–254 (1975)

    Google Scholar 

  • Brown, H.M., Ottoson, D.: Dual role for potassium inBalanus photoreceptor: antagonist of calcium and suppression of lightinduced current. J. Physiol. (Lond.)257, 355–378 (1976)

    Google Scholar 

  • Dörrscheidt-Käfer, M.: Das Empfindlichkeitsverhalten einzelner Photorezeptoren im Komplexauge der blauen SchmeiszfliegeCalliphora erythrocephala. Thesis, Ruhr-Universität Bochum (1971)

  • Dörrscheidt-Käfer, M.: Die Empfindlichkeit einzelner Photorezeptoren im Komplexauge vonCalliphora erythrocephala. J. Comp. Physiol.81, 309–340 (1972)

    Google Scholar 

  • Fulpius, B., Baumann, F.: Effects of sodium, potassium and calcium ions on slow and spike potentials in single photoreceptor cells. J. Gen. Physiol.53, 541–561 (1969)

    Google Scholar 

  • Fuortes, M.G.F.: Initiation of impulses in visual cells ofLimulus. J. Physiol. (Lond.)148, 14–28 (1959)

    Google Scholar 

  • Fuortes, M.G.F., Hodgkin, A.L.: Changes in time scale and sensitivity in the ommatidia ofLimulus. J. Physiol. (Lond.)172, 239–263 (1964)

    Google Scholar 

  • Hanani, M., Hillman, P.: Adaptation and facilitation in the barnacle photoreceptor. J. Gen. Physiol.67, 235–249 (1976)

    Google Scholar 

  • Hanani, M., Shaw, C.: A potassium contribution to the response of the barnacle photoreceptor. J. Physiol. (Lond.)270, 151–163 (1977)

    Google Scholar 

  • Koike, H., Brown, H.M., Hagiwara, S.: Hyperpolarization of a barnacle photoreceptor membrane following illumination. J. Gen. Physiol.57, 723–737 (1971)

    Google Scholar 

  • Lisman, J.E.: Effects of removing extracellular Ca2+ on excitation and adaptation inLimulus ventral photoreceptors. Biophys. J.16, 1331–1335 (1976)

    Google Scholar 

  • Lisman, J.E., Brown, J.E.: The effect of intracellular iontophoretic injection of calcium and sodium ions on the light response ofLimulus ventral photoreceptors. J. Gen. Physiol.59, 701–719 (1972)

    Google Scholar 

  • Lisman, J.E., Brown, J.E.: Light-induced changes of sensitivity inLimulus ventral photoreceptors. J. Gen. Physiol.66, 473–488 (1975a)

    Google Scholar 

  • Lisman, J.E., Brown, J.E.: Effects of intracellular injection of calcium buffers on light adaptation inLimulus ventral photoreceptors. J. Gen. Physiol.66, 489–506 (1975b)

    Google Scholar 

  • Millecchia, R., Mauro, A.: The ventral photoreceptor cells ofLimulus. II. The basic photoresponse. J. Gen. Physiol.54, 310–330 (1969a)

    Google Scholar 

  • Millecchia, R., Mauro, A.: The ventral photoreceptor cells ofLimulus. III. A voltage clamp study. J. Gen. Physiol.54, 331–351 (1969b)

    Google Scholar 

  • Muijser, H.: A micro-electrode amplifier with an infinite resistance current source for intracellular measurements of membrane potential and resistance changes under current clamp. Experientia (in press)

  • Muijser, H., Leutscher-Hazelhoff, J.T., Stavenga, D.G., Kuiper, J.W.: Photopigment conversions expressed in receptor potential and membrane resistance of blowfly visual sense cells. Nature254, 520–522 (1975)

    Google Scholar 

  • Pepose, J.S., Lisman, J.E.: Voltage-sensitive potassium channels inLimulus photoreceptors. J. Gen. Physiol.71, 101–120 (1978)

    Google Scholar 

  • Pinto, L.H., Brown, J.E.: Intracellular recordings from photoreceptors of the squid (Loligo pealii). J. Comp. Physiol.122, 241–250 (1977)

    Google Scholar 

  • Pinto, L.H., Pak, W.L.: Light-induced changes in photoreceptor membrane resistance and potential in Gecko. I. Preparations treated to reduce lateral interactions. J. Gen. Physiol.64, 26–48 (1974)

    Google Scholar 

  • Razmjoo, S., Hamdorf, K.: Visual sensitivity and the variation of total photopigment content in the blowfly photoreceptor membrane. J. Comp. Physiol.105, 279–286 (1976)

    Google Scholar 

  • Schmidt, J.A., Grossman, Y., Alkon, D.L.: Effects of external Ca on the photoresponse of a nudibranch mollusc. Biophys. J.21, 136a (1978).

    Google Scholar 

  • Stieve, H.: Experiments on the ionic mechanism of the receptor potential ofLimulus and crayfish photoreceptor. In: Biochemistry and physiology of visual pigments. Langer, H. (ed.), pp. 237–244. Berlin, Heidelberg, New York: Springer 1973

    Google Scholar 

  • Stieve, H.: On the ionic mechanisms responsible for the generation of the electrical response of light-sensitive cells. In: Biochemistry of sensory functions. Jaenicke, L. (ed.), pp. 79–105. Berlin, Heidelberg, New York: Springer 1974

    Google Scholar 

  • Stieve, H., Hanani, M.: Light and dark adaptation of crayfish visual cells depending on extracellular calcium concentration. Z. Naturforsch.31c, 324–327 (1976)

    Google Scholar 

  • Stieve, H., Malinowska, T., Sonneman, D.: Dependence of potassium ion and sodium exchange on light in the crayfish retina. Z. Naturforsch.29c, 754–763 (1974)

    Google Scholar 

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  1. Department of Biophysics, Laboratorium voor Algemene Natuurkunde, University Groningen, Groningen, The Netherlands

    H. Muijser

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Muijser, H. The receptor potential of retinular cells of the blowflyCalliphora: The role of sodium, potassium and calcium ions. J. Comp. Physiol. 132, 87–95 (1979). https://doi.org/10.1007/BF00617735

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