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The olfactoryL-serine receptor in coho salmon: biochemical specificity and behavioral response

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Summary

  1. 1.

    The specificity of the olfactory receptor site forl-serine in coho salmon,Oncorhynchus kisutch, was evaluated by using biochemical receptor and behavioral assays of olfaction.

  2. 2.

    A competitive binding experiment showed that the ability of amino acids to compete for thel-serine binding site depended on molecular size and charge.l-threonine,l-serine, andl-alanine were best able to compete withl-serine suggesting that the threonine-serine-alanine (TSA) odor binding site exists in coho salmon.

  3. 3.

    The behavioral response to singly-presented amino acids was evaluated by using a two-choice Y-trough. Threonine, serine, alanine, and histidine were behaviorally avoided at 10−7 M. All other amino acids tested were neither aversive nor attractive.

  4. 4.

    A cross-adaptation experiment indicated that the detection and behavioral avoidance of serine was inhibited by the adapting amino acids serine, alanine, or glycine but not by threonine, aspartic acid, or histidine.

  5. 5.

    A classical conditioning experiment showed that single fish were unable to discriminate between serine and alanine but were able to discriminate serine or alanine from a qualitatively different odor such as histidine.

  6. 6.

    These results, taken together, indicate thatl-serine andl-alanine were functionally identical odors to coho salmon. We propose that a functional olfactory receptor site for serine and alanine (site SA) exists in coho salmon.

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References

  • Anonymous (1967) Chemical attractants for the carp,Cyprinus carpio. Pacific Northwest Laboratories, Battelle Memorial Institute, Richland, Washington

  • Atema J (1980) Chemical senses, chemical signals, and feeding behavior in fishes. In: Bardach JE, Magnuson JJ, May RC, Reinhart JM (eds) Fish behavior and its use in the capture and culture of fishes. ICLARM Conference Proceedings 5, International Center for Living Aquatic Resources Management, Manila, Philippines, pp 57–101

    Google Scholar 

  • Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–252

    Google Scholar 

  • Brett JR, MacKinnon D (1954) Some aspects of olfactory perception in migrating adult coho and spring salmon. J Fish Res Board Can 11:310–318

    Google Scholar 

  • Brown SB, Hara TJ (1981) Accumulation of chemostimulatory amino acids by a sedimentable fraction isolated from olfactory rosettes of rainbow trout (Salmo gairdneri). Biochim Biophys Acta 675:149–162

    Google Scholar 

  • Brown SB, Hara TJ (1982) Biochemical aspects of amino acid receptors in olfaction and taste. In: Hara TJ (ed) Chemoreception in fishes. Elsevier, New York, pp 159–180

    Google Scholar 

  • Cagan RH, Zeiger WN (1978) Biochemical studies of olfaction: Binding specificity of radioactively labeled stimuli to an isolated olfactory preparation from rainbow trout (Salmo gairdneri). Proc Natl Acad Sci USA 75:4679–4683

    Google Scholar 

  • Cancalon P (1978) Isolation and characterization of the olfactory epithelial cells of the catfish. Chem Senses Flav 3:381–396

    Google Scholar 

  • Caprio J (1982) High sensitivity and specificity of olfactory and gustatory receptors of catfish to amino acids. In: Hara TJ (ed) Chemoreception in fishes. Elsevier, New York, pp 109–134

    Google Scholar 

  • Caprio J (1984) Olfaction and taste in fish. In: Bolis L, Keynes L, Maddress SHP (eds) Comparative physiology of sensory systems. Cambridge University Press, New York, pp 257–283

    Google Scholar 

  • Caprio J, Byrd RP (1984) Electrophysiological evidence for acidic, basic, and neutral amino acid olfactory receptor sites in the catfish. J Gen Physiol 84:403–422

    Google Scholar 

  • Carr WES, Blumenthal KM, Netherton JC (1977) Chemoreception in the pigfish,Orthopristis chrysopterus: The contribution of amino acids and betaine to stimulation of feeding behavior by various extracts. Comp Biochem Physiol 58A:69–73

    Google Scholar 

  • Fesenko EE, Novoselov VI, Krapivinskaya LD, Mjasoedov NF, Zolotarev JA (1983) Molecular mechanisms of odor sensing. VI. Some biochemical characteristics of a possible receptor for amino acids from the olfactory epithelium of the skateDasyatis pastinaca and carpCyprinus carpio. Biochim Biophys Acta 759:250–256

    Google Scholar 

  • Hamilton PB (1965) Amino-acids on hands. Nature 205:284–285

    Google Scholar 

  • Hara TJ (1976) Effects of pH on the olfactory responses to amino acids in rainbow trout,Salmo gairdneri. Comp Biochem Physiol 54A:37–39

    Google Scholar 

  • Hara TJ (1982) Structure-activity relationships of amino acids as olfactory stimuli. In: Hara TJ (ed) Chemoreception in fishes. Elsevier, New York, pp 135–158

    Google Scholar 

  • Hara TJ, Macdonald S, Evans RE, Marui T, Arai S (1984) Morpholine, bile acids, and skin mucus as possible chemical cues in salmonid homing: Electrophysiological re-evaluation. In: McCleave JD, Arnold GP, Dodson JJ, Neill WH (eds) Mechanisms of migration in fishes. NATO Conference Series IV, vol 14. Plenum Press, New York, pp 363–378

    Google Scholar 

  • Idler DR, Fagerlund UHM, Mayoh H (1956) Olfactory perception in migrating salmon. I.l-serine, a salmon repellent in mammalian skin. J Gen Physiol 39:889–892

    Google Scholar 

  • Neter J, Wasserman W (1974) Applied linear statistical models. Richard D. Irwin, Inc., Homewood, IL

    Google Scholar 

  • Novoselov VI, Krapivinskaya LD, Fesenko EE (1980) Molecular mechanisms of odor sensing. V. Some biochemical characteristics of the alanineous receptor from the olfactory epithelium of the skateDasyatis pastinaca. Chem Senses 5:195–203

    Google Scholar 

  • Ogata H, Murai T, Nose T (1983) Free amino acid composition in urine of carp and channel catfish. Bull Jpn Soc Sci Fish 49:1471

    Google Scholar 

  • Ohno T, Yoshii K, Kurihara K (1984) Multiple receptor types for amino acids in the carp olfactory cells revealed by quantitative cross-adaptation method. Brain Res 310:13–21

    Google Scholar 

  • Oro J, Skewes HB (1965) Free amino acids on human fingers: the question of contamination in microanalysis. Nature 207:1042–1045

    Google Scholar 

  • Pawson MG (1977) Analysis of a natural chemical attractant for whitingMerlangius merlangus L. and codGadus morhua L. using a behavioral bioassay. Comp Biochem Physiol 56A:129–135

    Google Scholar 

  • Rehnberg BG, Schreck CB (1986) Acute metal toxicology of olfaction in coho salmon: Behavior, receptors, and odormetal complexation. Bull Environ Contam Toxicol 36:579–586

    Google Scholar 

  • Rehnberg BG, Jonasson B, Schreck CB (1985) Olfactory sensitivity during parr and smolt developmental stages of coho salmon. Trans Am Fish Soc 114:732–736

    Google Scholar 

  • Rhein LD, Cagan RH (1983) Biochemical studies of olfaction: binding specificity of odorants to a cilia preparation from rainbow trout olfactory rosetts. J Neurochem 41:569–577

    Google Scholar 

  • Shparkovskiy IA, Pavlov ID, Troshicheva NV, Klets DV (1981) Response of pink salmon,Oncorhynchus gorbuscha, and Atlantic salmon,Salmo salar, to amino acids during spawning migration. J Ichthyol 21:135–140

    Google Scholar 

  • Snoeyink VL, Jenkins D (1980) Water chemistry. John Wiley & Sons, New York

    Google Scholar 

  • Sokal RR, Rohlf FJ (1981) Biometry. Freeman & Co., San Francisco

    Google Scholar 

  • Stabell OB, Selset R (1980) Comparison of mucus collecting methods in fish olfaction. Acta Physiol Scand 108:91–96

    Google Scholar 

  • Sutterlin AM (1975) Chemical attraction of some marine fish in their natural habitat. J Fish Res Board Can 32:729–738

    Google Scholar 

  • Tucker D (1983) Fish chemoreception: peripheral anatomy and physiology. In: Northcutt RG, Davis RE (ed) Fish neurobiology, vol 1, Brain stem and sense organs. University of Michigan Press, Ann Arbor, pp 311–349

    Google Scholar 

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Author notes

  1. Bradley G. Rehnberg

    Present address: Department of Biology, University of Saskatchewan, S7N OWO, Saskatoon, Canada

Authors and Affiliations

  1. Oregon Cooperative Fishery Research Unit, Department of Fisheries & Wildlife, Oregon State University, 97331, Corvallis, Oregon, USA

    Bradley G. Rehnberg & Carl B. Schreck

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  1. Bradley G. Rehnberg
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  2. Carl B. Schreck
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Additional information

Cooperators are Oregon State University, Oregon Department of Fish and Wildlife, and US Fish and Wildlife Service

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Rehnberg, B.G., Schreck, C.B. The olfactoryL-serine receptor in coho salmon: biochemical specificity and behavioral response. J. Comp. Physiol. 159, 61–67 (1986). https://doi.org/10.1007/BF00612496

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