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Olfactory receptors of the spiny lobster: ATP-sensitive cells with similarities to P2-type purinoceptors of vertebrates

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Summary

  1. 1.

    A distinct population of ATP-sensitive cells, with response characteristics indicative of P2-type purinoceptors found in internal tissues of vertebrates, was identified among the antennular olfactory cells of the spiny lobster,Panulirus argus.

  2. 2.

    Extracellular recordings from single cells showed that the ATP-sensitive cells had the following properties in common with P2 purinoceptors: a) potency sequence of ATP>ADP>AMP and adenosine; b) broad sensitivity to nucleotide triphosphates including those with modifications in both the purine and ribose moieties; c) stimulated by slowly degradable analogs of ATP, namely,β, γ-imido ATP (AMPPNP),β, γ-methylene ATP (AMPPCP), and α,β-methylene ATP (AMPCPP).

  3. 3.

    The activity sequence of the ATP-sensitive cells for nucleotides and related substances was ATP≥2′-deoxyATP>GTP>CTP≥XTP≥ITP> 8-bromo-ATP=ADP, with pyrophosphate, AMP, and tripolyphosphate being virtually inactive.

  4. 4.

    The potency sequence for the slowly degradable analogs was AMPPNP>ATP = AMPPCP> AMPCPP.

  5. 5.

    Differences in structure-activity relationships, response duration, and response magnitude clearly distinguished the ATP-sensitive cells from another type of olfactory purinoceptor, the AMP-sensitive cells, also occurring in the antennules of the lobster.

  6. 6.

    Comparisons between the ATP-sensitive chemoreceptors of the lobster and of certain insects revealed similarities in the activity sequence of ATP, ADP, AMP, and certain other nucleotides. However differences existed in the relative potencies of ATP, AMPPNP, and AMPPCP, and in the relative inactivity of inorganic pyrophosphate in the lobster.

  7. 7.

    The findings of this study lend additional credence to the earlier hypothesis that receptors for transmitters and modulators existing in internal tissues may have evolved from external chemoreceptors of primitive unicellular organisms.

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Authors and Affiliations

  1. C.V. Whitney Marine Laboratory and Department of Zoology, University of Florida, 32086, St. Augustine, Florida, USA

    William E. S. Carr, Richard A. Gleeson, Barry W. Ache & Marsha Lynn Milstead

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  1. William E. S. Carr
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  2. Richard A. Gleeson
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  3. Barry W. Ache
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  4. Marsha Lynn Milstead
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Carr, W.E.S., Gleeson, R.A., Ache, B.W. et al. Olfactory receptors of the spiny lobster: ATP-sensitive cells with similarities to P2-type purinoceptors of vertebrates. J. Comp. Physiol. 158, 331–338 (1986). https://doi.org/10.1007/BF00603616

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  • DOI: https://doi.org/10.1007/BF00603616

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