Summary
The86Rb efflux from isolated Sepia retina incubated in artificial sea water was raised 1.7–4.8 fold upon illumination with light of intensities ranging from 5×1012 to 5.6×1015 effective photons/cm2/sec. There was a simultaneous fall of 6% in the rubidium content.
Addition of retinaldehyde (10−4M) or dinitrofluorobenzene (2×10−3 M) gave 45% and 35% increases in the dark efflux rate. Retinol (10−4 M) gave a smaller effect and retinoic acid (10−4 M) was without effect.
The presence of ouabain (10−4 M) reduced the effect of light on the efflux rate. This effect could be explained by the decreased rubidium concentration gradient across the membrane.
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de Pont, J.J.H.H.M., Duncan, G. & Bonting, S.L. Biochemical aspects of the visual process. Pflugers Arch. 322, 278–286 (1971). https://doi.org/10.1007/BF00602075
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DOI: https://doi.org/10.1007/BF00602075