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Modification of sturgeon protamines with succinic anhydride

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Abstract

The modification of the α- and ε-NH2 groups of sturins A and B — protamines fromAcipenser güldenstadti — with succinic anhydride has been studied. Succinylation was carried out at 20°C, pH from 7.5 to 10.5, using a 50 to 180-fold excess of succinic anhydride. The modification products were separated by reversed-phase HPLC on a Zorbax ODS column. A linear relationship has been established between the retention times of the succinyl derivatives and the number of succinyl groups introduced. The influence of the pH and of the excess of succinic anhydride on the modification of the sturins has been studied by the method of experimental planning and mathematical modeling. The optimum conditions have been found for the succinylation of the proteins: a 150- to 180-fold excess of succinic anhydride and pH values of 9.8–10.2 for sturin A and of 8.4–9.4 for sturin B.

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Literature cited

  1. S. Taylor and I. Folkman, Nature (London),297, 307 (1982).

    Google Scholar 

  2. N. M. Kutsenko, Z. A. Kuzina, and L. E. Alekseeva, Antibiotics and Medical Biotechnology [in Russian] (1985), p. 592.

  3. A. Trouet, M. Masquelier, R. Baurain, and D. Depres-De Campeneere, Proc. Natl. Acad. Sci. USA,79, 626 (1982).

    Google Scholar 

  4. E. P. Yulikova, L. K. Evseenko, L. A. Baratova, L. P. Belyanova, V. K. Rybin, and A. B. Silaev, Bioorg. Khim.,2, 1613 (1976).

    Google Scholar 

  5. A. N. Bulanova and E. P. Yulikova, Khim. Prir. Soedin., 76 (1980).

  6. A. Gounaris and G. Perlamann, J. Biol. Chem.,242, 2739 (1967).

    Google Scholar 

  7. A. Bernard, N. A. Nieto, A. Viogue, and E. Palacian, Biochem. Biophys. Acta,873, 350 (1986).

    Google Scholar 

  8. V. K. Rybin, N. N. Yaglitskaya, T. Yu. Matrosovich, N. V. Makarov, Khim. Prir. Soedin., 744 (1986).

  9. V. K. Rybin and E. P. Yulikova, Biokhimiya,46, 276 (1981).

    Google Scholar 

  10. O. L. Davis and P. L. Goldsmith, Statistical Methods in Research and Production, 4th edn., Longman, London (1980).

    Google Scholar 

  11. A. Arellano, M. Canales, C. Jullian, and J. E. Brunet, Biochem. Biophys. Res. Commun.,150, 633 (1988).

    Google Scholar 

  12. E. P. Yulikova, V. K. Rybin, A. B. Silaev, Biorg. Khim.,5, 5 (1979).

    Google Scholar 

  13. K. Satake and J. Luck, Bull. Soc. Chim. Biol.,40, 1743 (1958).

    Google Scholar 

  14. M. Sokolovsky and B. L. Vallee, Biochemistry,5, 3574 (1966).

    Google Scholar 

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Authors
  1. V. K. Rybin
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  2. A. Georgopulos
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  3. N. V. Makarov
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  4. G. S. Katrukha
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Additional information

Moscow Technological Institute of the Meat and Dairy Industry. All-Union Scientific-Research Institute for the Search for New Antibiotics, Moscow. Translated from Khimiya Prirodnykh Soedinenii, No. 2, pp. 238–243, March–April, 1989.

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Rybin, V.K., Georgopulos, A., Makarov, N.V. et al. Modification of sturgeon protamines with succinic anhydride. Chem Nat Compd 25, 205–209 (1989). https://doi.org/10.1007/BF00598413

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  • DOI: https://doi.org/10.1007/BF00598413

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