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Detection of areas of wall differentiation in fungi using fluorescent staining

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Abstract

Fungal colonies were stained with a fluorescent brightner, Calcofluor White M2R New. When viewed using ultraviolet light certain hyphal regions showed intense fluorescence, whereas others showed a lower intensity fluorescence.

The apical 10 μm of leadingBotrytis cinerea hyphae showed intense fluorescence. Developing septa and side branches also showed this intense localised fluorescence.

Calcofluor staining of hyphae and their subsequent growth illustrated the phenomenon of tip extension.

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Author notes

  1. Keith Gull

    Present address: The Biological Laboratory, University of Kent, Canterbury, Kent, England

Authors and Affiliations

  1. Microbiology Department, Queen Elizabeth College, Campden Hill Road, Kensington, London

    Keith Gull & Anthony P. J. Trinci

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  1. Keith Gull
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  2. Anthony P. J. Trinci
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Gull, K., Trinci, A.P.J. Detection of areas of wall differentiation in fungi using fluorescent staining. Arch. Microbiol. 96, 53–57 (1974). https://doi.org/10.1007/BF00590162

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  • DOI: https://doi.org/10.1007/BF00590162

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