Summary
We have identified tight linkage of an RFLP marker to theHt1 gene of maize that confers resistance to the fungal pathogenHelminthosporium turcicum race 1. This was accomplished by the use of four pairs of near isogenic lines (NILs; B73, A619, W153R, and CM105), each differing by the presence or the absence of the geneHt1. SinceHt1 maps to chromosome 2, 26 clones already mapped to this chromosome were labeled and probed against Southern blots of these NILs DNA digested with three restriction enzymes:EcoRI,BamHI, andHindIII. Six markers exhibited an RFLP for at least one pair of NILs. Presumptive linkage was further tested by analyzing the segregation of five of the six markers (one was monomorphic in the cross studied) and resistance toH. turcicum race 1 on 95 F2 individuals from the cross DF20 × LH146Ht. The results indicate a tight linkage between one of the DNA markers,UMC150B, and theHt1 gene.
Similar content being viewed by others
References
Allard RW (1956) Formulas and tables to facilitate the calculation of recombination values in heredity. Hilgardia 24:235–277
Beckmann JS, Soller M (1986) Restriction fragment length polymorphisms in plant genetic improvement. Oxford Surv Plant Mol Cell Biol 3:197–250
Coe Jr EH, Neuffer MG, Hoisington DA (1988) The genetics of corn. In: Sprague GF, Dudley JW (eds) Corn and corn improvement, 3rd edn. American Society of Agronomy Crop Science Society of America Soil Science Society of America Publishers, Madison/WI, pp 81–258
Ellis JG, Lawrence GJ, Peacock WJ, Pryor AJ (1988) Approaches to cloning plant genes conferring resistance to fungal pathogens. Annu Rev Phytopathol 26:245–263
Feinberg AP, Volgelstein B (1983) A technique for radiolabeling DNA restriction fragments to a high specific activity. Anal Biochem 132:6–13
Gevers HO (1975) A new major gene for resistance toHelminthosporium turcicum leaf blight of maize. Plant Dis Rep 59:296–299
Haldane JBS (1919) The combination of linkage values and the calculation of distance between the loci of linked factors. J Genet 8:299–309
Hanson WD (1959) Early generation analysis of lengths of heterozygous chromosome segments around a locus held heterozygous with backcrossing or selfing. Genetics 44:833–837
Helentjaris T, Slocum M, Wright S, Schaefer A, Nienhuis J (1986) Construction of genetic linkage maps in maize and tomato using restriction fragment length polymorphisms. Theor Appl Genet 72:761–769
Hilu HM, Hooker AL (1963) Monogenic chlorotic lesion resistance toHelminthosporium turcicum in corn seedlings. Phytopathology 53:909–912
Hoisington DA, Coe EH (1989a) Maping in maize using RFLPs. In: Gustafson JP (ed) Stadler Genetics Symposium-Gene Manipulation in Plant Improvement II. Plenum Press, New York London, pp 331–352
Hoisington DA, Coe EH (1989b) Methods for correlating RFLP maps with conventional genetic and physical maps in maize. In: Helentjaris T, Burr B (eds) Development and application of molecular markers to problems in plant genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor/NY, pp 19–24
Hooker AL (1963) Monogenic resistance inZea mays L. toHelminthosporium turcicum. Crop Sci 3:381–383
Hooker AL (1977) A second major gene locus in corn for chlorotic lesion resistance toHelminthosporium turcicum. Crop Sci 17:132–135
Hooker AL (1981) Resistance toHelminthosporium turcicum fromTripsacum floridanum incorporated into corn. Maize Genetics Cooperation news letter 55:87–88
Hooker AL, Kim SK (1973) Monogenic and multigenic resistance toHelminthosporium turcicum in corn. Plant Dis Rep 57:586–589
Hughes GR, Hooker AL (1971) Gene action conditioning resistance to northern leaf blight in maize. Crop Sci 11:180–183
Jenkins MT, Robert AL (1952) Inheritance of resistance to the leaf blight of corn caused byHelminthosporium turcicum. Agron J 44:136–140
Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L (1987) MAPMAKER: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181
Perkins JM, Hooker AL (1981) Reactions of eighty-four sources of chlorotic lesion resistance in corn to three biotypes ofHelminthosporium turcicum. Plant Dis 65:502–504
Young ND, Tanksley SD (1989) RFLP analysis of the size of chromosomal segments retained around theTm-2 locus of tomato during backcross breeding. Theor Appl Genet 77:353–359
Young ND, Zamir D, Ganal MW, Tanksley SD (1988) Use of isogenic lines and simultaneous probing to identify DNA markers tightly linked to theTm-2a gene in tomato. Genetics 120:579–585
Author information
Authors and Affiliations
Additional information
Communicated by J. Beckmann
Rights and permissions
About this article
Cite this article
Bentolila, S., Guitton, C., Bouvet, N. et al. Identification of an RFLP marker tightly linked to theHt1 gene in maize. Theoret. Appl. Genetics 82, 393–398 (1991). https://doi.org/10.1007/BF00588588
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00588588